TSH3 Antibody - #DF10052

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Product: TSH3 Antibody
Catalog: DF10052
Description: Rabbit polyclonal antibody to TSH3
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 119 kDa; 119kD(Calculated).
Uniprot: Q63HK5
RRID: AB_2840632

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(83%), Bovine(92%), Horse(100%), Sheep(83%), Rabbit(83%), Dog(92%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
TSH3 Antibody detects endogenous levels of total TSH3.
RRID:
AB_2840632
Cite Format: Affinity Biosciences Cat# DF10052, RRID:AB_2840632.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA1474; Teashirt homolog 3; TSH3; TSH3_HUMAN; TSHZ3; Zinc finger protein 537;

Immunogens

Immunogen:
Uniprot:

Q63HK5(TSH3_HUMAN) >>Visit HPA database.

Gene(ID):
TSHZ3(57616)
Expression:
Q63HK5 TSH3_HUMAN:

Expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease (PubMed:18776146, PubMed:19343227). Expressed in the fetal neocortex (PubMed:27668656).

Sequence:
MPRRKQQAPRRAAAYVSEELKAAALVDEGLDPEEHTADGEPSAKYMCPEKELARACPSYQNSPAAEFSCHEMDSESHISETSDRMADFESGSIKNEEETKEVTVPLEDTTVSDSLEQMKAVYNNFLSNSYWSNLNLNLHQPSSEKNNGSSSSSSSSSSSCGSGSFDWHQSAMAKTLQQVSQSRMLPEPSLFSTVQLYRQSSKLYGSIFTGASKFRCKDCSAAYDTLVELTVHMNETGHYRDDNHETDNNNPKRWSKPRKRSLLEMEGKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPLKEPVTPVAAKIIPATRKKASLELELPSSPDSTGGTPKATISDTNDALQKNSNPYITPNNRYGHQNGASYAWHFEARKSQILKCMECGSSHDTLQELTAHMMVTGHFIKVTNSAMKKGKPIVETPVTPTITTLLDEKVQSVPLAATTFTSPSNTPASISPKLNVEVKKEVDKEKAVTDEKPKQKDKPGEEEEKCDISSKYHYLTENDLEESPKGGLDILKSLENTVTSAINKAQNGTPSWGGYPSIHAAYQLPNMMKLSLGSSGKSTPLKPMFGNSEIVSPTKNQTLVSPPSSQTSPMPKTNFHAMEELVKKVTEKVAKVEEKMKEPDGKLSPPKRATPSPCSSEVGEPIKMEASSDGGFRSQENSPSPPRDGCKDGSPLAEPVENGKELVKPLASSLSGSTAIITDHPPEQPFVNPLSALQSVMNIHLGKAAKPSLPALDPMSMLFKMSNSLAEKAAVATPPPLQSKKADHLDRYFYHVNNDQPIDLTKGKSDKGCSLGSVLLSPTSTAPATSSSTVTTAKTSAVVSFMSNSPLRENALSDISDMLKNLTESHTSKSSTPSSISEKSDIDGATLEEAEESTPAQKRKGRQSNWNPQHLLILQAQFAASLRQTSEGKYIMSDLSPQERMHISRFTGLSMTTISHWLANVKYQLRRTGGTKFLKNLDTGHPVFFCNDCASQIRTPSTYISHLESHLGFRLRDLSKLSTEQINSQIAQTKSPSEKMVTSSPEEDLGTSYQCKLCNRTFASKHAVKLHLSKTHGKSPEDHLLYVSELEKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Chicken
100
Bovine
92
Dog
92
Xenopus
92
Pig
83
Sheep
83
Rabbit
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q63HK5 As Substrate

Site PTM Type Enzyme
T36 Phosphorylation
S68 Phosphorylation
S92 Phosphorylation
S112 Phosphorylation
S114 Phosphorylation
S151 Phosphorylation
S152 Phosphorylation
S153 Phosphorylation
S154 Phosphorylation
S164 Phosphorylation
S200 Phosphorylation
S201 Phosphorylation
T209 Phosphorylation
K213 Ubiquitination
S261 Phosphorylation
K302 Ubiquitination
T310 Phosphorylation
S325 Phosphorylation
S333 Phosphorylation
K421 Acetylation
K423 Acetylation
T428 Phosphorylation
T431 Phosphorylation
T433 Phosphorylation
T436 Phosphorylation
S463 Phosphorylation
K503 Ubiquitination
S515 Phosphorylation
S584 Phosphorylation
S596 Phosphorylation
T599 Phosphorylation
S600 Phosphorylation
S636 Phosphorylation
S647 Phosphorylation
S648 Phosphorylation
S672 Phosphorylation
S682 Phosphorylation
S809 Phosphorylation
S818 Phosphorylation
T824 Phosphorylation
S835 Phosphorylation
S837 Phosphorylation
S845 Phosphorylation
S857 Phosphorylation
T859 Phosphorylation
S860 Phosphorylation
S862 Phosphorylation
S863 Phosphorylation
S928 Phosphorylation
S947 Phosphorylation
S1007 Phosphorylation
S1016 Phosphorylation
S1023 Phosphorylation

Research Backgrounds

Function:

Transcriptional regulator involved in developmental processes. Functions in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter (By similarity). Involved in the modulation of glutamatergic synaptic transmission and long-term synaptic potentiation (By similarity).

Subcellular Location:

Nucleus. Cell projection>Growth cone.
Note: Colocalizes with APBB1 in axonal growth cone (By similarity). Colocalizes with APBB1 in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease. Expressed in the fetal neocortex.

Subunit Structure:

Interacts (via homeobox domain) with APBB1 (via PID domain 1). Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3.

Family&Domains:

Belongs to the teashirt C2H2-type zinc-finger protein family.

Restrictive clause

 

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