TRIM68 Antibody | Affinity Biosciences

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TRIM68 Antibody - Western blot analysis of TRIM68 using HT-29 whole cell lysates.

Product:	TRIM68 Antibody
Catalog:	DF10055
Description:	Rabbit polyclonal antibody to TRIM68
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep
Mol.Wt.:	56 kDa; 56kD(Calculated).
Uniprot:	Q6AZZ1
RRID:	AB_2840635

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Bovine(100%), Horse(88%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

TRIM68 Antibody detects endogenous levels of total TRIM68.

RRID:

AB_2840635
Cite Format: Affinity Biosciences Cat# DF10055, RRID:AB_2840635.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

E3 ubiquitin-protein ligase TRIM68; FLJ10369; GC109; MGC126176; RING finger protein 137; RNF 137; RNF137; Ro/SSA1 related protein; SS 56; SS-56; SS56; SSA protein SS 56; SSA protein SS-56; TRI68_HUMAN; TRIM 68; Trim68; Tripartite motif containing 68; Tripartite motif containing protein 68; Tripartite motif-containing protein 68;

Immunogens

Immunogen:

Uniprot:

Q6AZZ1(TRI68_HUMAN) >>Visit HPA database.

Gene(ID):

TRIM68(55128)

Expression:

Q6AZZ1 TRI68_HUMAN:

Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells.

Sequence:

Q6AZZ1 TRI68_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPTALVEAIVEEVACPICMTFLREPMSIDCGHSFCHSCLSGLWEIPGESQNWGYTCPLCRAPVQPRNLRPNWQLANVVEKVRLLRLHPGMGLKGDLCERHGEKLKMFCKEDVLIMCEACSQSPEHEAHSVVPMEDVAWEYKWELHEALEHLKKEQEEAWKLEVGERKRTATWKIQVETRKQSIVWEFEKYQRLLEKKQPPHRQLGAEVAAALASLQREAAETMQKLELNHSELIQQSQVLWRMIAELKERSQRPVRWMLQDIQEVLNRSKSWSLQQPEPISLELKTDCRVLGLREILKTYAADVRLDPDTAYSRLIVSEDRKRVHYGDTNQKLPDNPERFYRYNIVLGSQCISSGRHYWEVEVGDRSEWGLGVCKQNVDRKEVVYLSPHYGFWVIRLRKGNEYRAGTDEYPILSLPVPPRRVGIFVDYEAHDISFYNVTDCGSHIFTFPRYPFPGRLLPYFSPCYSIGTNNTAPLAICSLDGED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Pig

Horse

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6AZZ1 As Substrate

Q6AZZ1

Site	PTM Type	Source
K81	Ubiquitination	Uniprot
K94	Ubiquitination	Uniprot
K104	Ubiquitination	Uniprot
K153	Ubiquitination	Uniprot
K161	Ubiquitination	Uniprot
T170	Phosphorylation	Uniprot
T172	Phosphorylation	Uniprot
T179	Phosphorylation	Uniprot
K181	Ubiquitination	Uniprot
S232	Phosphorylation	Uniprot
S238	Phosphorylation	Uniprot
K271	Ubiquitination	Uniprot
K286	Ubiquitination	Uniprot
K299	Ubiquitination	Uniprot
K333	Ubiquitination	Uniprot

Research Backgrounds

Q6AZZ1_TRI68_HUMAN

Function:

Functions as a ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.

PTMs:

Auto-ubiquitinated.

Subcellular Location:

Cytoplasm>Perinuclear region. Nucleus.
Note: Colocalized with AR in nucleus.

Tissue Specificity:

Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells.

Subunit Structure:

Interacts with AR/androgen receptor (via ligand-binding domain). Interacts with KAT5/TIP60.

Family&Domains:

The RING domain is essential for ubiquitin E3 ligase activity.

Belongs to the TRIM/RBCC family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TRIM68 Antibody - #DF10055