ZNF227 Antibody - #DF10061
(3)
| Product: | ZNF227 Antibody |
| Catalog: | DF10061 |
| Description: | Rabbit polyclonal antibody to ZNF227 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Dog |
| Mol.Wt.: | 92 kDa; 92kD(Calculated). |
| Uniprot: | Q86WZ6 |
| RRID: | AB_2840641 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Dog(83%)
Clonality:
Polyclonal
Specificity:
ZNF227 Antibody detects endogenous levels of total ZNF227.
RRID:
AB_2840641
Cite Format: Affinity Biosciences Cat# DF10061, RRID:AB_2840641.
Cite Format: Affinity Biosciences Cat# DF10061, RRID:AB_2840641.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Zinc finger protein 227; ZN227_HUMAN; ZNF227;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q86WZ6 ZN227_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSQNYDLPQKKQEKMTKFQEAVTFKDVAVVFSREELRLLDLTQRKLYRDVMVENFKNLVAVGHLPFQPDMVSQLEAEEKLWMMETETQRSSKHQNKMETLQKFALKYLSNQELSCWQIWKQVASELTRCLQGKSSQLLQGDSIQVSENENNIMNPKGDSSIYIENQEFPFWRTQHSCGNTYLSESQIQSRGKQIDVKNNLQIHEDFMKKSPFHEHIKTDTEPKPCKGNEYGKIISDGSNQKLPLGEKPHPCGECGRGFSYSPRLPLHPNVHTGEKCFSQSSHLRTHQRIHPGEKLNRCHESGDCFNKSSFHSYQSNHTGEKSYRCDSCGKGFSSSTGLIIHYRTHTGEKPYKCEECGKCFSQSSNFQCHQRVHTEEKPYKCEECGKGFGWSVNLRVHQRVHRGEKPYKCEECGKGFTQAAHFHIHQRVHTGEKPYKCDVCGKGFSHNSPLICHRRVHTGEKPYKCEACGKGFTRNTDLHIHFRVHTGEKPYKCKECGKGFSQASNLQVHQNVHTGEKRFKCETCGKGFSQSSKLQTHQRVHTGEKPYRCDVCGKDFSYSSNLKLHQVIHTGEKPYKCEECGKGFSWRSNLHAHQRVHSGEKPYKCEQCDKSFSQAIDFRVHQRVHTGEKPYKCGVCGKGFSQSSGLQSHQRVHTGEKPYKCDVCGKGFRYSSQFIYHQRGHTGEKPYKCEECGKGFGRSLNLRHHQRVHTGEKPHICEECGKAFSLPSNLRVHLGVHTREKLFKCEECGKGFSQSARLEAHQRVHTGEKPYKCDICDKDFRHRSRLTYHQKVHTGKKL
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Dog
83
Pig
78
Bovine
56
Sheep
56
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86WZ6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T174 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| K198 | Sumoylation | Uniprot | |
| K209 | Sumoylation | Uniprot | |
| K233 | Sumoylation | Uniprot | |
| K242 | Sumoylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| R264 | Methylation | Uniprot | |
| K295 | Sumoylation | Uniprot | |
| Y343 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| T347 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K353 | Sumoylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K378 | Sumoylation | Uniprot | |
| K381 | Sumoylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K409 | Sumoylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| T431 | Phosphorylation | Uniprot | |
| K434 | Sumoylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| T459 | Phosphorylation | Uniprot | |
| K462 | Sumoylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| T487 | Phosphorylation | Uniprot | |
| K490 | Sumoylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K534 | Sumoylation | Uniprot | |
| T543 | Phosphorylation | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| T571 | Phosphorylation | Uniprot | |
| K574 | Ubiquitination | Uniprot | |
| K577 | Acetylation | Uniprot | |
| K577 | Sumoylation | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| T627 | Phosphorylation | Uniprot | |
| K630 | Sumoylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| T655 | Phosphorylation | Uniprot | |
| K658 | Sumoylation | Uniprot | |
| K658 | Ubiquitination | Uniprot | |
| K689 | Sumoylation | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| K745 | Sumoylation | Uniprot | |
| T767 | Phosphorylation | Uniprot | |
| K770 | Sumoylation | Uniprot | |
| K770 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
May be involved in transcriptional regulation.
Subcellular Location:
Nucleus.
Family&Domains:
Belongs to the krueppel C2H2-type zinc-finger protein family.
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