C16orf44 Antibody | Affinity Biosciences

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C16orf44 Antibody - Western blot analysis of C16orf44 using HUVEC whole cell lysates.

C16orf44 Antibody - DF10066 at 1/100 staining human ovarian cancer by IHC-P.

Product:	C16orf44 Antibody
Catalog:	DF10066
Description:	Rabbit polyclonal antibody to C16orf44
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	69 kDa; 70kD(Calculated).
Uniprot:	Q8N4N3
RRID:	AB_2840646

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Zebrafish(82%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(91%), Dog(91%), Chicken(82%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

C16orf44 Antibody detects endogenous levels of total C16orf44.

RRID:

AB_2840646
Cite Format: Affinity Biosciences Cat# DF10066, RRID:AB_2840646.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ12543; Kelch like 36; Kelch like protein 36; kelch-like 36 (Drosophila); Kelch-like protein 36; KLH36_HUMAN; KLHL 36; Klhl36;

Immunogens

Immunogen:

Uniprot:

Q8N4N3(KLH36_HUMAN) >>Visit HPA database.

Gene(ID):

KLHL36(79786)

Sequence:

Q8N4N3 KLH36_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMEGSRQTRVSRPYKISESSKVYRWADHSSTVLQRLNEQRLRGLFCDVVLVADEQRVPAHRNLLAVCSDYFNSMFTIGMREAFQKEVELIGASYIGLKAVVDFLYGGELVLDGGNIDYVLETAHLLQIWTVVDFCCEYLEQEVSEDNYLYLQELASIYSLKRLDAFIDGFILNHFGTLSFTPDFLQNVSMQKLCVYLSSSEVQRECEHDLLQAALQWLTQQPEREAHARQVLENIHFPLIPKNDLLHRVKPAVCSLLPKEANCEGFIEEAVRYHNNLAAQPVMQTKRTALRTNQERLLFVGGEVSERCLELSDDTCYLDAKSEQWVKETPLPARRSHHCVAVLGGFIFIAGGSFSRDNGGDAASNLLYRYDPRCKQWIKVASMNQRRVDFYLASIEDMLVAIGGRNENGALSSVETYSPKTDSWSYVAGLPRFTYGHAGTIYKDFVYISGGHDYQIGPYRKNLLCYDHRTDVWEERRPMTTARGWHSMCSLGDSIYSIGGSDDNIESMERFDVLGVEAYSPQCNQWTRVAPLLHANSESGVAVWEGRIYILGGYSWENTAFSKTVQVYDREADKWSRGVDLPKAIAGGSACVCALEPRPEDKKKKGKGKRHQDRGQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Horse

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N4N3 As Substrate

Q8N4N3

Site	PTM Type	Source
S17	Phosphorylation	Uniprot
S19	Phosphorylation	Uniprot
S93	Phosphorylation	Uniprot
Y94	Phosphorylation	Uniprot
S199	Phosphorylation	Uniprot
S200	Phosphorylation	Uniprot
K286	Ubiquitination	Uniprot
K327	Ubiquitination	Uniprot
K420	Ubiquitination	Uniprot
T440	Phosphorylation	Uniprot
K607	Acetylation	Uniprot

Research Backgrounds

Q8N4N3_KLH36_HUMAN

Function:

Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Subunit Structure:

Interacts with CUL3.

Restrictive clause

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For Research Use Only.
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C16orf44 Antibody - #DF10066