LZTR1 Antibody - #DF10067

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Product: LZTR1 Antibody
Catalog: DF10067
Description: Rabbit polyclonal antibody to LZTR1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Xenopus
Mol.Wt.: 95 kDa; 95kD(Calculated).
Uniprot: Q8N653
RRID: AB_2840647

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
LZTR1 Antibody detects endogenous levels of total LZTR1.
RRID:
AB_2840647
Cite Format: Affinity Biosciences Cat# DF10067, RRID:AB_2840647.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Leucine zipper like regulator 1; Leucine zipper like transcription regulator 1; Leucine-zipper-like transcriptional regulator 1; LZTR-1; Lztr1; LZTR1_HUMAN; MGC21205; TCFL2;

Immunogens

Immunogen:
Uniprot:

Q8N653(LZTR1_HUMAN) >>Visit HPA database.

Gene(ID):
LZTR1(8216)
Sequence:
MAGPGSTGGQIGAAALAGGARSKVAPSVDFDHSCSDSVEYLTLNFGPFETVHRWRRLPPCDEFVGARRSKHTVVAYKDAIYVFGGDNGKTMLNDLLRFDVKDCSWCRAFTTGTPPAPRYHHSAVVYGSSMFVFGGYTGDIYSNSNLKNKNDLFEYKFATGQWTEWKIEGRLPVARSAHGATVYSDKLWIFAGYDGNARLNDMWTIGLQDRELTCWEEVAQSGEIPPSCCNFPVAVCRDKMFVFSGQSGAKITNNLFQFEFKDKTWTRIPTEHLLRGSPPPPQRRYGHTMVAFDRHLYVFGGAADNTLPNELHCYDVDFQTWEVVQPSSDSEVGGAEVPERACASEEVPTLTYEERVGFKKSRDVFGLDFGTTSAKQPTQPASELPSGRLFHAAAVISDAMYIFGGTVDNNIRSGEMYRFQFSCYPKCTLHEDYGRLWESRQFCDVEFVLGEKEECVQGHVAIVTARSRWLRRKITQARERLAQKLEQEAAPVPREAPGVAAGGARPPLLHVAIREAEARPFEVLMQFLYTDKIKYPRKGHVEDVLLIMDVYKLALSFQLCRLEQLCRQYIEASVDLQNVLVVCESAARLQLSQLKEHCLNFVVKESHFNQVIMMKEFERLSSPLIVEIVRRKQQPPPRTPLDQPVDIGTSLIQDMKAYLEGAGAEFCDITLLLDGHPRPAHKAILAARSSYFEAMFRSFMPEDGQVNISIGEMVPSRQAFESMLRYIYYGEVNMPPEDSLYLFAAPYYYGFYNNRLQAYCKQNLEMNVTVQNVLQILEAADKTQALDMKRHCLHIIVHQFTKVSKLPTLRSLSQQLLLDIIDSLASHISDKQCAELGADI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N653 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K101 Ubiquitination
T111 Phosphorylation
T113 Phosphorylation
Y119 Phosphorylation
Y141 Phosphorylation
K149 Ubiquitination
K166 Ubiquitination
S244 Phosphorylation
S247 Phosphorylation
K261 Ubiquitination
T270 Phosphorylation
R275 Methylation
Y352 Phosphorylation
K375 Ubiquitination
K426 Ubiquitination
K484 Ubiquitination
K615 Ubiquitination
K682 Ubiquitination
Y726 Phosphorylation

Research Backgrounds

Function:

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates ubiquitination of Ras (K-Ras/KRAS, N-Ras/NRAS and H-Ras/HRAS). Is a negative regulator of RAS-MAPK signaling that acts by controlling Ras levels and decreasing Ras association with membranes.

PTMs:

Phosphorylated on tyrosine upon induction of apoptosis, leading to its degradation by the proteasome.

Subcellular Location:

Endomembrane system. Recycling endosome. Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Component of the BCR(LZTR1) E3 ubiquitin ligase complex, at least composed of CUL3, LZTR1 and RBX1. Interacts with Ras (K-Ras/KRAS, N-Ras/NRAS and H-Ras/HRAS). Interacts with RAF1. Interacts with SHOC2. Interacts with PPP1CB.

Family&Domains:

Belongs to the LZTR1 family.

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