PKCB1 Antibody - #AF0502

(3)  
Product: PKCB1 Antibody
Catalog: AF0502
Description: Rabbit polyclonal antibody to PKCB1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Dog
Mol.Wt.: 132kDa; 132kD(Calculated).
Uniprot: Q9ULU4
RRID: AB_2834345

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
PKCB1 Antibody detects endogenous levels of total PKCB1.
RRID:
AB_2834345
Cite Format: Affinity Biosciences Cat# AF0502, RRID:AB_2834345.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CTCL tumor antigen se14 3; Cutaneous T cell lymphoma associated antigen se14 3; KIAA1125; MGC31836; predicted protein of HQ2893; PRKCBP 1; PRO2893; Protein kinase C binding protein 1; Rack7; Zinc finger MYND domain containing protein 8; ZMYND 8; ZMYND8;

Immunogens

Immunogen:
Uniprot:

Q9ULU4(PKCB1_HUMAN) >>Visit HPA database.

Gene(ID):
ZMYND8(23613)
Expression:
Q9ULU4 PKCB1_HUMAN:

Expressed in all tissues examined with highest expression in brain, lung, pancreas, and placenta. Expressed in cutaneous T-cell lymphomas (CTCL).

Description:
The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.
Sequence:
MDISTRSKDPGSAERTAQKRKFPSPPHSSNGHSPQDTSTSPIKKKKKPGLLNSNNKEQSELRHGPFYYMKQPLTTDPVDVVPQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLRLTSEPEGDWFCPECEKITVAECIETQSKAMTMLTIEQLSYLLKFAIQKMKQPGTDAFQKPVPLEQHPDYAEYIFHPMDLCTLEKNAKKKMYGCTEAFLADAKWILHNCIIYNGGNHKLTQIAKVVIKICEHEMNEIEVCPECYLAACQKRDNWFCEPCSNPHPLVWAKLKGFPFWPAKALRDKDGQVDARFFGQHDRAWVPINNCYLMSKEIPFSVKKTKSIFNSAMQEMEVYVENIRRKFGVFNYSPFRTPYTPNSQYQMLLDPTNPSAGTAKIDKQEKVKLNFDMTASPKILMSKPVLSGGTGRRISLSDMPRSPMSTNSSVHTGSDVEQDAEKKATSSHFSASEESMDFLDKSTASPASTKTGQAGSLSGSPKPFSPQLSAPITTKTDKTSTTGSILNLNLDRSKAEMDLKELSESVQQQSTPVPLISPKRQIRSRFQLNLDKTIESCKAQLGINEISEDVYTAVEHSDSEDSEKSDSSDSEYISDDEQKSKNEPEDTEDKEGCQMDKEPSAVKKKPKPTNPVEIKEELKSTSPASEKADPGAVKDKASPEPEKDFSEKAKPSPHPIKDKLKGKDETDSPTVHLGLDSDSESELVIDLGEDHSGREGRKNKKEPKEPSPKQDVVGKTPPSTTVGSHSPPETPVLTRSSAQTSAAGATATTSTSSTVTVTAPAPAATGSPVKKQRPLLPKETAPAVQRVVWNSSSKFQTSSQKWHMQKMQRQQQQQQQQNQQQQPQSSQGTRYQTRQAVKAVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVNADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSDHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Dog
91
Bovine
82
Rabbit
45
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULU4 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
S24 Phosphorylation
S28 Phosphorylation
S33 Phosphorylation
T37 Phosphorylation
S40 Phosphorylation
K46 Acetylation
S53 Phosphorylation
K56 Acetylation
T235 Phosphorylation
K294 Ubiquitination
K356 Ubiquitination
Y362 Phosphorylation
S363 Phosphorylation
Y369 Phosphorylation
T404 Phosphorylation
S406 Phosphorylation
S412 Phosphorylation
K413 Acetylation
S417 Phosphorylation
T420 Phosphorylation
S425 Phosphorylation
S427 Phosphorylation
S432 Phosphorylation
S435 Phosphorylation
T436 Phosphorylation
S438 Phosphorylation
S439 Phosphorylation
T442 Phosphorylation
S444 Phosphorylation
T455 Phosphorylation
S456 Phosphorylation
S460 Phosphorylation
S462 Phosphorylation
S465 Phosphorylation
S472 Phosphorylation
S475 Phosphorylation
T479 Phosphorylation
T481 Phosphorylation
S486 Phosphorylation
S488 Phosphorylation
S490 Phosphorylation
S495 Phosphorylation
S499 Phosphorylation
K508 Ubiquitination
S514 Phosphorylation
S523 Phosphorylation
S535 Phosphorylation
S540 Phosphorylation
T541 Phosphorylation
S547 Phosphorylation
R555 Methylation
T563 Phosphorylation
S587 Phosphorylation
S589 Phosphorylation
S592 Phosphorylation
S595 Phosphorylation
S610 Phosphorylation
K611 Sumoylation
S630 Phosphorylation
T639 Phosphorylation
K645 Sumoylation
S652 Phosphorylation
S655 Phosphorylation
S668 Phosphorylation
S676 Phosphorylation
S682 Phosphorylation
T696 Phosphorylation
S707 Phosphorylation
S709 Phosphorylation
S711 Phosphorylation
S737 Phosphorylation
T746 Phosphorylation
T750 Phosphorylation
S754 Phosphorylation
S756 Phosphorylation
T760 Phosphorylation
T764 Phosphorylation
S797 Phosphorylation
S1086 Phosphorylation
S1089 Phosphorylation
T1093 Phosphorylation
S1114 Phosphorylation
S1119 Phosphorylation
S1124 Phosphorylation
S1141 Phosphorylation
S1152 Phosphorylation

Research Backgrounds

Function:

May act as a transcriptional corepressor for KDM5D. Required for KDM5D-mediated down-regulation of diverse metastasis-associated genes; the function seems to involve the recognition of the dual histone signature H3K4me1-H3K14ac. Suppresses prostate cancer cell invasion.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Sequestered in the cytoplasm through the interaction with DBN1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined with highest expression in brain, lung, pancreas, and placenta. Expressed in cutaneous T-cell lymphomas (CTCL).

Subunit Structure:

Interacts in vitro with PRKCB1. Interacts with KDM5D and KDM1A. Interacts with histone H3 (via N-terminus) that is both methylated at 'Lys-4' (H3K4me1) and acetylated at 'Lys-14' (H3K14ac), and with histone H3 (via N-terminus) unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-14' (H3K14ac). Interacts (via PHD-type Zinc finger, Bromo and PWWP domains) with DBN1 (via ADF-H domain); the interaction leads to sequestering of ZMYND8 in the cytoplasm.

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