MINA Antibody - #DF10071

(2)  
Product: MINA Antibody
Catalog: DF10071
Description: Rabbit polyclonal antibody to MINA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 53 kDa; 53kD(Calculated).
Uniprot: Q8IUF8
RRID: AB_2840651

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(86%), Bovine(86%), Horse(100%), Sheep(86%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MINA Antibody detects endogenous levels of total MINA.
RRID:
AB_2840651
Cite Format: Affinity Biosciences Cat# DF10071, RRID:AB_2840651.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L27a histidine hydroxylase; Bifunctional lysine specific demethylase and histidyl hydroxylase MINA; FLJ14393; Histone lysine demethylase MINA; MDIG; MINA; MINA_HUMAN; Mineral dust-induced gene protein; Myc induced nuclear antigen 53 kDa; MYC induced nuclear antigen; MYC-induced nuclear antigen; NO52; Nucleolar protein 52; Ribosomal oxygenase MINA; ROX;

Immunogens

Immunogen:
Uniprot:

Q8IUF8(RIOX2_HUMAN) >>Visit HPA database.

Gene(ID):
RIOX2(84864)
Expression:
Q8IUF8 RIOX2_HUMAN:

Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.

Sequence:
MPKKAKPTGSGKEEGPAPCKQMKLEAAGGPSALNFDSPSSLFESLISPIKTETFFKEFWEQKPLLIQRDDPALATYYGSLFKLTDLKSLCSRGMYYGRDVNVCRCVNGKKKVLNKDGKAHFLQLRKDFDQKRATIQFHQPQRFKDELWRIQEKLECYFGSLVGSNVYITPAGSQGLPPHYDDVEVFILQLEGEKHWRLYHPTVPLAREYSVEAEERIGRPVHEFMLKPGDLLYFPRGTIHQADTPAGLAHSTHVTISTYQNNSWGDFLLDTISGLVFDTAKEDVELRTGIPRQLLLQVESTTVATRRLSGFLRTLADRLEGTKELLSSDMKKDFIMHRLPPYSAGDGAELSTPGGKLPRLDSVVRLQFKDHIVLTVLPDQDQSDEAQEKMVYIYHSLKNSRETHMMGNEEETEFHGLRFPLSHLDALKQIWNSPAISVKDLKLTTDEEKESLVLSLWTECLIQVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
86
Sheep
86
Zebrafish
86
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IUF8 As Substrate

Site PTM Type Enzyme
K12 Acetylation
K20 Acetylation
S47 Phosphorylation
K56 Ubiquitination
K62 Ubiquitination
K82 Methylation
K82 Ubiquitination
K87 Methylation
K87 Ubiquitination
K118 Ubiquitination
K144 Ubiquitination
S210 Phosphorylation
S309 Phosphorylation
R318 Methylation
K323 Ubiquitination
K331 Ubiquitination
K332 Acetylation
K332 Ubiquitination
K356 Acetylation
K356 Ubiquitination
K428 Ubiquitination
K439 Ubiquitination
K442 Ubiquitination

Research Backgrounds

Function:

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.

Subcellular Location:

Nucleus. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.

Family&Domains:

Belongs to the ROX family. MINA53 subfamily.

Restrictive clause

 

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