MYCBP Antibody | Affinity Biosciences

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MYCBP Antibody - DF10076 staining Hela cells by IF/ICC.

Product:	MYCBP Antibody
Catalog:	DF10076
Description:	Rabbit polyclonal antibody to MYCBP
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	12 kDa; 12kD(Calculated).
Uniprot:	Q99417
RRID:	AB_2840656

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

MYCBP Antibody detects endogenous levels of total MYCBP.

RRID:

AB_2840656
Cite Format: Affinity Biosciences Cat# DF10076, RRID:AB_2840656.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AMY 1; AMY-1; AMY1; Associate of Myc 1; C myc binding protein; C-Myc-binding protein; Cmyc binding protein; MYCBP; MYCBP_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q99417(MYCBP_HUMAN) >>Visit HPA database.

Gene(ID):

MYCBP(26292)

Expression:

Q99417 MYCBP_HUMAN:

Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.

Sequence:

Q99417 MYCBP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAHYKAADSKREQFRRYLEKSGVLDTLTKVLVALYEEPEKPNSALDFLKHHLGAATPENPEIELLRLELAEMKEKYEAIVEENKKLKAKLAQYEPPQEEKRAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Dog

100

Rabbit

100

Xenopus

Chicken

Pig

Horse

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99417 As Substrate

Q99417

Site	PTM Type	Source
K5	Ubiquitination	Uniprot
K20	Acetylation	Uniprot
K20	Ubiquitination	Uniprot
K29	Ubiquitination	Uniprot
K40	Ubiquitination	Uniprot
T56	Phosphorylation	Uniprot
K89	Ubiquitination	Uniprot
K100	Ubiquitination	Uniprot

Research Backgrounds

Q99417_MYCBP_HUMAN

Function:

May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.

Subcellular Location:

Cytoplasm. Nucleus. Mitochondrion.
Note: Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.

Tissue Specificity:

Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.

Subunit Structure:

Binds via its C-terminal region to the N-terminal region of MYC. Associates with AKAP1/S-AKAP84. Interacts with MYCBPAP.

Family&Domains:

Belongs to the AMY1 family.

References

1). Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. PLOS Neglected Tropical Diseases, 2017 (PubMed: 36206314) [IF=3.4]

Application: WB Species: Mouse Sample:

Fig 1. The dynamic changes of mouse proteins in serum-derived exosomes in response to E. multilocularis infection. (A) and (B) Transmission electron microscopy of the enriched extracellular vesicles (EVs) isolated from the sera of E. multilocularis-infected (A) and uninfected mice (B). (C) The diameter distribution of the purified EVs. (D) Western blotting analysis of mammalian EV-marker CD63 in the purified EVs. (E) Heatmap of differentially abundance proteins between the serum exosomes from E. multilocularis-infected and uninfected mice. (F) Western blotting analysis the abundance of TGF-β1, GPATC8, LRP1, and AMY1 during E. multilocularis infection. https://doi.org/10.1371/journal.pntd.0010814.g001

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MYCBP Antibody - #DF10076