ZNF148 Antibody - #AF0792

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Product: ZNF148 Antibody
Catalog: AF0792
Description: Rabbit polyclonal antibody to ZNF148
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 89kDa; 89kD(Calculated).
Uniprot: Q9UQR1
RRID: AB_2834348

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
ZNF148 Antibody detects endogenous levels of total ZNF148.
RRID:
AB_2834348
Cite Format: Affinity Biosciences Cat# AF0792, RRID:AB_2834348.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Transcription factor ZBP-89; Transcription factor ZBP89; ZBP89; Zinc finger DNA binding protein 89; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148_HUMAN; ZNF 148; ZNF148;

Immunogens

Immunogen:

A synthesized peptide derived from human ZNF148, corresponding to a region within N-terminal amino acids.

Uniprot:

Q9UQR1(ZN148_HUMAN) >>Visit HPA database.

Gene(ID):
ZNF148(7707)
Description:
ZNF148 a transcription factor. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
Sequence:
MNIDDKLEGLFLKCGGIDEMQSSRTMVVMGGVSGQSTVSGELQDSVLQDRSMPHQEILAADEVLQESEMRQQDMISHDELMVHEETVKNDEEQMETHERLPQGLQYALNVPISVKQEITFTDVSEQLMRDKKQIREPVDLQKKKKRKQRSPAKILTINEDGSLGLKTPKSHVCEHCNAAFRTNYHLQRHVFIHTGEKPFQCSQCDMRFIQKYLLQRHEKIHTGEKPFRCDECGMRFIQKYHMERHKRTHSGEKPYQCEYCLQYFSRTDRVLKHKRMCHENHDKKLNRCAIKGGLLTSEEDSGFSTSPKDNSLPKKKRQKTEKKSSGMDKESALDKSDLKKDKNDYLPLYSSSTKVKDEYMVAEYAVEMPHSSVGGSHLEDASGEIHPPKLVLKKINSKRSLKQPLEQNQTISPLSTYEESKVSKYAFELVDKQALLDSEGNADIDQVDNLQEGPSKPVHSSTNYDDAMQFLKKKRYLQAASNNSREYALNVGTIASQPSVTQAAVASVIDESTTASILESQALNVEIKSNHDKNVIPDEVLQTLLDHYSHKANGQHEISFSVADTEVTSSISINSSEVPEVTPSENVGSSSQASSSDKANMLQEYSKFLQQALDRTSQNDAYLNSPSLNFVTDNQTLPNQPAFSSIDKQVYATMPINSFRSGMNSPLRTTPDKSHFGLIVGDSQHSFPFSGDETNHASATSTQDFLDQVTSQKKAEAQPVHQAYQMSSFEQPFRAPYHGSRAGIATQFSTANGQVNLRGPGTSAEFSEFPLVNVNDNRAGMTSSPDATTGQTFG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Xenopus
80
Chicken
75
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.

PTMs:

Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

References

1). Hsa_circRNA_0101125 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma via miR-143-3p/ZNF148 Axis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2025 (PubMed: 41020579) [IF=4.4]

Application: WB    Species: human    Sample: KYSE150 and ECA109 cell

FIGURE 2. Effect of hsa_circRNA_0101125 knockdown on proliferation, migration, and epithelial marker expression in ESCC cells. (A) RT‐qPCR analysis showing the reduction of hsa_circRNA_0101125 expression in KYSE150 and ECA109 cells after transfection with sh‐hsa_circRNA_0101125. (B) RT‐qPCR analysis of miR‐143‐3p expression following hsa_circRNA_0101125 knockdown. (C) Representative images of scratch wound healing assay in ECA109 cells at 0 h and 24 h. (D‐E) Quantitative analysis of wound closure. (F) Cell viability of ECA109 cells measured by CCK‐8 assay at 24 h and 48 h after knockdown. (G) Western blot analysis of E‐cadherin protein expression in KYSE150 and ECA109 cells after knockdown. Relative expression values represent the gray intensity of E‐cadherin normalized to β‐Actin, based on densitometry analysis using ImageJ software. *p 

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