IFI6 Antibody | Affinity Biosciences

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Product:	IFI6 Antibody
Catalog:	DF10115
Description:	Rabbit polyclonal antibody to IFI6
Application:	WB
Reactivity:	Human
Mol.Wt.:	13 kDa; 13kD(Calculated).
Uniprot:	P09912
RRID:	AB_2840695

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Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

IFI6 Antibody detects endogenous levels of total IFI6.

RRID:

AB_2840695
Cite Format: Affinity Biosciences Cat# DF10115, RRID:AB_2840695.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Ifi-6-16; IFI6; IFI6_HUMAN; Interferon alpha-inducible protein 6; Interferon-induced protein 6-16;

Immunogens

Immunogen:

Uniprot:

P09912(IFI6_HUMAN) >>Visit HPA database.

Gene(ID):

IFI6(2537)

Sequence:

P09912 IFI6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRQKAVSLFLCYLLLFTCSGVEAGKKKCSESSDSGSGFWKALTFMAVGGGLAVAGLPALGFTGAGIAANSVAASLMSWSAILNGGGVPAGGLVATLQSLGAGGSSVVIGNIGALMGYATHKYLDSEEDEE

PTMs - P09912 As Substrate

P09912

Site	PTM Type	Enzyme	Source
K27	Ubiquitination		Uniprot
S125	Phosphorylation		Uniprot

Research Backgrounds

P09912_IFI6_HUMAN

Function:

Plays a role in apoptosis, negatively regulating the intrinsinc apoptotic signaling pathway and TNFSF10-induced apoptosis. However, it has also been shown to have a pro-apoptotic activity. Has an antiviral activity towards hepatitis C virus/HCV by inhibiting the EGFR signaling pathway, which activation is required for entry of the virus into cells.

PTMs:

Glycosylated.

Subcellular Location:

Mitochondrion inner membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts with CIB1; the interaction is direct.

Family&Domains:

Belongs to the IFI6/IFI27 family.

References

1). Annexin A2 (ANXA2) regulates the transcription and alternative splicing of inflammatory genes in renal tubular epithelial cells. BMC Genomics, 2022 [IF=3.5]

Application: WB Species: Human Sample: HK2 cells

Fig. 2ANXA2 regulated inflammatory gene mRNA and protein expression in HK2 cells. A Top ten GO biological processes terms enriched by upregulated DEGs in shANXA2 cells vs shCtrl cells. B Top ten KEGG functional pathways enriched by upregulated DEGs in shANXA2 cells vs shCtrl cells. C Top ten KEGG functional pathways enriched by downregulated DEGs in shANXA2 cells vs shCtrl cells. D Validation of mRNA expression of CCL5, IFI6, IFI44, IFITM1,and LTB by qRT-PCR assay. E Validation of mRNA expression of IRF7 and ISG15 by qRT-PCR assay. F Representative images showing protein levels of ANXA2, CCL5, IFI6, IFI44, IFITM1, LTB, IRF7 and ISG15 in LV-shANXA2 group vs LV-shCtrl group. Results are represented as mean ± SD.(n = 3,* P

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For Research Use Only.
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IFI6 Antibody - #DF10115