Product: KRT84 Antibody
Catalog: DF10116
Description: Rabbit polyclonal antibody to KRT84
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 65 kDa; 65kD(Calculated).
Uniprot: Q9NSB2
RRID: AB_2840696

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(86%), Bovine(86%), Horse(86%), Sheep(86%), Dog(86%)
Clonality:
Polyclonal
Specificity:
KRT84 Antibody detects endogenous levels of total KRT84.
RRID:
AB_2840696
Cite Format: Affinity Biosciences Cat# DF10116, RRID:AB_2840696.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hard keratin, type II, 4; HB4; K84; keratin 84; Keratin; keratin, hair, basic, 4; keratin, type II cuticular Hb4; Keratin-84; KRT84; KRT84_HUMAN; KRTHB4; type II cuticular Hb4; type II hair keratin 4; Type II hair keratin Hb4; type II keratin Kb24; Type-II keratin Kb24;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9NSB2 KRT84_HUMAN:

Expressed in the hair follicles.

Sequence:
MSCRSYRVSSGHRVGNFSSCSAMTPQNLNRFRANSVSCWSGPGFRGLGSFGSRSVITFGSYSPRIAAVGSRPIHCGVRFGAGCGMGFGDGRGVGLGPRADSCVGLGFGAGSGIGYGFGGPGFGYRVGGVGVPAAPSITAVTVNKSLLTPLNLEIDPNAQRVKKDEKEQIKTLNNKFASFIDKVRFLEQQNKLLETKWSFLQEQKCIRSNLEPLFESYITNLRRQLEVLVSDQARLQAERNHLQDVLEGFKKKYEEEVVCRANAENEFVALKKDVDAAFMNKSDLEANVDTLTQEIDFLKTLYMEEIQLLQSHISETSVIVKMDNSRDLNLDGIIAEVKAQYEEVARRSRADAEAWYQTKYEEMQVTAGQHCDNLRNIRNEINELTRLIQRLKAEIEHAKAQRAKLEAAVAEAEQQGEATLSDAKCKLADLECALQQAKQDMARQLCEYQELMNAKLGLDIEIATYRRLLEGEESRLCEGVGPVNISVSSSRGGLVCGPEPLVAGSTLSRGGVTFSGSSSVCATSGVLASCGPSLGGARVAPATGDLLSTGTRSGSMLISEACVPSVPCPLPTQGGFSSCSGGRSSSVRFVSTTTSCRTKY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Horse
86
Bovine
86
Sheep
86
Dog
86
Rabbit
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NSB2 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K163 Ubiquitination
K166 Ubiquitination
K170 Ubiquitination
T171 Phosphorylation
K175 Acetylation
K175 Methylation
K175 Sumoylation
K175 Ubiquitination
S178 Phosphorylation
K182 Acetylation
K182 Ubiquitination
K191 Ubiquitination
Y341 Phosphorylation
Y360 Phosphorylation
K438 Sumoylation
K455 Methylation
S474 Phosphorylation

Research Backgrounds

Tissue Specificity:

Expressed in the hair follicles.

Subunit Structure:

Heterotetramer of two type I and two type II keratins.

Family&Domains:

Belongs to the intermediate filament family.

Restrictive clause

 

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