MCM8 Antibody - #DF10122
| Product: | MCM8 Antibody |
| Catalog: | DF10122 |
| Description: | Rabbit polyclonal antibody to MCM8 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Rat |
| Prediction: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 94 kDa; 94kD(Calculated). |
| Uniprot: | Q9UJA3 |
| RRID: | AB_2840702 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF10122, RRID:AB_2840702.
Fold/Unfold
C20orf154; dJ967N21.5; DNA helicase MCM8; DNA replication licensing factor MCM8; MCM8; MCM8_HUMAN; MGC119522; MGC119523; MGC12866; MGC4816; Minichromosome maintenance 8; Minichromosome maintenance complex component 8; REC;
Immunogens
Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers.
- Q9UJA3 MCM8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNGEYRGRGFGRGRFQSWKRGRGGGNFSGKWREREHRPDLSKTTGKRTSEQTPQFLLSTKTPQSMQSTLDRFIPYKGWKLYFSEVYSDSSPLIEKIQAFEKFFTRHIDLYDKDEIERKGSILVDFKELTEGGEVTNLIPDIATELRDAPEKTLACMGLAIHQVLTKDLERHAAELQAQEGLSNDGETMVNVPHIHARVYNYEPLTQLKNVRANYYGKYIALRGTVVRVSNIKPLCTKMAFLCAACGEIQSFPLPDGKYSLPTKCPVPVCRGRSFTALRSSPLTVTMDWQSIKIQELMSDDQREAGRIPRTIECELVHDLVDSCVPGDTVTITGIVKVSNAEEGSRNKNDKCMFLLYIEANSISNSKGQKTKSSEDGCKHGMLMEFSLKDLYAIQEIQAEENLFKLIVNSLCPVIFGHELVKAGLALALFGGSQKYADDKNRIPIRGDPHILVVGDPGLGKSQMLQAACNVAPRGVYVCGNTTTTSGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSENLKMGSALLSRFDLVFILLDTPNEHHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDPIPHQLLRKYIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELREEATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UJA3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R6 | Methylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| Y201 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| K569 | Ubiquitination | Uniprot | |
| K571 | Ubiquitination | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| T619 | Phosphorylation | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| K668 | Ubiquitination | Uniprot | |
| S715 | Phosphorylation | Uniprot | |
| K734 | Ubiquitination | Uniprot |
Research Backgrounds
Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Probably by regulating HR, plays a key role during gametogenesis (By similarity). Stabilizes MCM9 protein.
Nucleus. Chromosome.
Note: Localizes to nuclear foci (PubMed:26215093). Localizes to double-stranded DNA breaks (PubMed:23401855). Binds chromatin throughout the cell cycle (PubMed:15684404).
Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers.
Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9. Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites. Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1. Interacts with CDC6 and ORC2.
Belongs to the MCM family.
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