NEK3 Antibody - #DF10137
| Product: | NEK3 Antibody |
| Catalog: | DF10137 |
| Description: | Rabbit polyclonal antibody to NEK3 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig |
| Mol.Wt.: | 58 kDa; 58kD(Calculated). |
| Uniprot: | P51956 |
| RRID: | AB_2840717 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF10137, RRID:AB_2840717.
Fold/Unfold
HSPK 36; NEK3; NEK3_HUMAN; Never in mitosis A-related kinase 3; NIMA (never in mitosis gene a) related kinase 3; NimA related protein kinase 3; NimA-related protein kinase 3; Serine/threonine protein kinase Nek3; Serine/threonine-protein kinase Nek3;
Immunogens
Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain.
- P51956 NEK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDYMVLRMIGEGSFGRALLVQHESSNQMFAMKEIRLPKSFSNTQNSRKEAVLLAKMKHPNIVAFKESFEAEGHLYIVMEYCDGGDLMQKIKQQKGKLFPEDMILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQGCISPLPSHYSYELQFLVKQMFKRNPSHRPSATTLLSRGIVARLVQKCLPPEIIMEYGEEVLEEIKNSKHNTPRKKTNPSRIRIALGNEASTVQEEEQDRKGSHTDLESINENLVESALRRVNREEKGNKSVHLRKASSPNLHRRQWEKNVPNTALTALENASILTSSLTAEDDRGGSVIKYSKNTTRKQWLKETPDTLLNILKNADLSLAFQTYTIYRPGSEGFLKGPLSEETEASDSVDGGHDSVILDPERLEPGLDEEDTDFEEEDDNPDWVSELKKRAGWQGLCDR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51956 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| T288 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot | |
| R435 | Methylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot |
Research Backgrounds
Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.
Phosphorylation at Thr-479 regulates its catalytic activity.
Cytoplasm. Cell projection>Axon.
Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain.
Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent.
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
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