SIK2 Antibody | Affinity Biosciences

WB
IF/ICC

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SIK2 Antibody - Western blot analysis SNF1LK2 using HT-29 whole cell lysates.

SIK2 Antibody - DF10140 staining HepG2 cells by IF/ICC.

Product:	SIK2 Antibody
Catalog:	DF10140
Description:	Rabbit polyclonal antibody to SIK2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	104 kDa; 104kD(Calculated).
Uniprot:	Q9H0K1
RRID:	AB_2840720

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(83%), Horse(100%), Sheep(83%), Rabbit(91%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SIK2 Antibody detects endogenous levels of total SIK2.

RRID:

AB_2840720
Cite Format: Affinity Biosciences Cat# DF10140, RRID:AB_2840720.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp434K1115; EC 2.7.11.1; KIAA0781; LOH11CR1I; QIK; Qin induced kinase; Qin-induced kinase; Salt inducible kinase 2; Salt inducible protein kinase 2; Salt inducible serine/threonine kinase 2; Salt-inducible protein kinase 2; Serine/threonine protein kinase SIK2; Serine/threonine protein kinase SNF1 like kinase 2; Serine/threonine-protein kinase SIK2; Serine/threonine-protein kinase SNF1-like kinase 2; SIK 2; SIK-2; SIK2; SIK2_HUMAN; SNF1 like kinase; SNF1 like kinase 2; SNF1LK2;

Immunogens

Immunogen:

A synthesized peptide derived from human SIK2, corresponding to a region within the internal amino acids.

Uniprot:

Q9H0K1(SIK2_HUMAN) >>Visit HPA database.

Gene(ID):

SIK2(23235)

Sequence:

Q9H0K1 SIK2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDTPKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLANQPSPRMTSPFISLRPTNPAMQALSSQKREVHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADPNLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPAPLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Pig

Rabbit

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q9H0K1_SIK2_HUMAN

Function:

Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.

PTMs:

Phosphorylated at Thr-175 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.

Acetylation at Lys-53 inhibits kinase activity. Deacetylated by HDAC6.

Subcellular Location:

Cytoplasm.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Research Fields

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Fluoride Stimulates Anxiety- and Depression-like Behaviors Associated with SIK2-CRTC1 Signaling Dysfunction. Journal of Agricultural and Food Chemistry, 2021 (PubMed: 34735150) [IF=5.7]

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SIK2 Antibody - #DF10140