NRBP1 Antibody - #DF10146

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Product: NRBP1 Antibody
Catalog: DF10146
Description: Rabbit polyclonal antibody to NRBP1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 60 kDa; 60kD(Calculated).
Uniprot: Q9UHY1
RRID: AB_2840726

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NRBP1 Antibody detects endogenous levels of total NRBP1.
RRID:
AB_2840726
Cite Format: Affinity Biosciences Cat# DF10146, RRID:AB_2840726.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BCON3; MADM; MUDPNP; Multiple domain putative nuclear protein; Myeloid leukemia factor 1 adaptor molecule; NRBP; NRBP_HUMAN; NRBP1; Nuclear receptor binding protein; Nuclear receptor-binding protein;

Immunogens

Immunogen:
Uniprot:

Q9UHY1(NRBP_HUMAN) >>Visit HPA database.

Gene(ID):
NRBP1(29959)
Expression:
Q9UHY1 NRBP_HUMAN:

Ubiquitously expressed in all tissues examined with high levels in the testis.

Sequence:
MSEGESQTVLSSGSDPKVESSSSAPGLTSVSPPVTSTTSAASPEEEEESEDESEILEESPCGRWQKRREEVNQRNVPGIDSAYLAMDTEEGVEVVWNEVQFSERKNYKLQEEKVRAVFDNLIQLEHLNIVKFHKYWADIKENKARVIFITEYMSSGSLKQFLKKTKKNHKTMNEKAWKRWCTQILSALSYLHSCDPPIIHGNLTCDTIFIQHNGLIKIGSVAPDTINNHVKTCREEQKNLHFFAPEYGEVTNVTTAVDIYSFGMCALEMAVLEIQGNGESSYVPQEAISSAIQLLEDPLQREFIQKCLQSEPARRPTARELLFHPALFEVPSLKLLAAHCIVGHQHMIPENALEEITKNMDTSAVLAEIPAGPGREPVQTLYSQSPALELDKFLEDVRNGIYPLTAFGLPRPQQPQQEEVTSPVVPPSVKTPTPEPAEVETRKVVLMQCNIESVEEGVKHHLTLLLKLEDKLNRHLSCDLMPNENIPELAAELVQLGFISEADQSRLTSLLEETLNKFNFARNSTLNSAAVTVSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHY1 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S6 Phosphorylation
T8 Phosphorylation
S11 Phosphorylation
S12 Phosphorylation
S14 Phosphorylation
S49 Phosphorylation
K134 Acetylation
K134 Ubiquitination
Y152 Phosphorylation
T225 Phosphorylation
K231 Ubiquitination
T232 Phosphorylation
K306 Ubiquitination
S363 Phosphorylation
K392 Ubiquitination
S422 Phosphorylation
S428 Phosphorylation
K430 Ubiquitination
T431 Phosphorylation
T433 Phosphorylation
K471 Ubiquitination
K517 Ubiquitination
S535 Phosphorylation

Research Backgrounds

Function:

May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.

Subcellular Location:

Cytoplasm>Cell cortex. Endomembrane system. Cell projection>Lamellipodium.
Note: Colocalizes with activated RAC3 to endomembranes and at the cell periphery in lamellipodia.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in all tissues examined with high levels in the testis.

Subunit Structure:

Homodimer. Binds to MLF1, recruiting a serine kinase which phosphorylates both itself and MLF1. Phosphorylated MLF1 binds to YWHAZ and is retained in the cytoplasm (By similarity).

Family&Domains:

The protein kinase domain is predicted to be catalytically inactive.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

References

1). NRBP1 modulates uric acid transporter ABCG2 expression by activating the Wnt/β-catenin pathway in HK-2 cells. Nefrología (English Edition), 2022 (PubMed: 36437206)

Application: WB    Species: Human    Sample: HK-2 cells

Fig. 2. Effect of NRBP1 overexpression and knockdown on the secretion and reabsorption of uric acid. A, Effect of NRBP1 overexpression and knockdown on the mRNA expression of NRBP1, ABCG2, OAT1, GLUT9 and URAT1. B, Effect of NRBP1 overexpression and knockdown on the protein expression of NRBP1, ABCG2, OAT1, GLUT9 and URAT1.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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