AKAP2 Antibody - #AF0651

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Product: AKAP2 Antibody
Catalog: AF0651
Description: Rabbit polyclonal antibody to AKAP2
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig
Mol.Wt.: 96kDa; 95kD(Calculated).
Uniprot: Q9Y2D5
RRID: AB_2834353

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(82%)
Clonality:
Polyclonal
Specificity:
AKAP2 Antibody detects endogenous levels of total AKAP2.
RRID:
AB_2834353
Cite Format: Affinity Biosciences Cat# AF0651, RRID:AB_2834353.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A kinase (PRKA) anchor protein 2; A kinase anchor protein 2; AKAP 2; AKAP KL; AKAPKL; KIAA0920; PALM 2; PALM2; PRKA 2; PRKA2; Protein kinase A anchoring protein 2; Protein kinase A2;

Immunogens

Immunogen:
Uniprot:

Q9Y2D5(AKAP2_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP2(11217)
Description:
AKAP2 Binds to regulatory subunit (RII) of protein kinase A. May be involved in establishing polarity in signaling systems or in integrating PKA-RII isoforms with downstream effectors to capture, amplify and focus diffuse, trans-cellular signals carried by cAMP. 5 isoforms of the human protein are produced by alternative splicing.
Sequence:
MEIEVSVAECKSVPGITSTPHPMDHPSAFYSPPHNGLLTDHHESLDNDVAREIRYLDEVLEANCCDSAVDGTYNGTSSPEPGAVVLVGGLSPPVHEATQPEPTERTASRQAPPHIELSNSSPDPMAEAERTNGHSPSQPRDALGDSLQVPVSPSSTTSSRCSSRDGEFTLTTLKKEAKFELRAFHEDKKPSKLFEDDEHEKEQYCIRKVRPSEEMLELEKERRELIRSQAVKKNPGIAAKWWNPPQEKTIEEQLDEEHLESHKKYKERKERRAQQEQLLLQKQLQQQQQQPPSQLCTAPASSHERASMIDKAKEDIVTEQIDFSAARKQFQLMENSRQAVAKGQSTPRLFSIKPFYRPLGSVNSDKPLTNPRPPSVGGPPEDSGASAAKGQKSPGALETPSAAGSQGNTASQGKEGPYSEPSKRGPLSKLWAEDGEFTSARAVLTVVKDDDHGILDQFSRSVNVSLTQEELDSGLDELSVRSQDTTVLETLSNDFSMDNISDSGASNETTNALQENSLADFSLPQTPQTDNPSEGRGEGVSKSFSDHGFYSPSSTLGDSPLVDDPLEYQAGLLVQNAIQQAIAEQVDKAVSKTSRDGAEQQGPEATVEEAEAAAFGSEKPQSMFEPPQVSSPVQEKRDVLPKILPAEDRALRERGPPQPLPAVQPSGPINMEETRPEGSYFSKYSEAAELRSTASLLATQESDVMVGPFKLRSRKQRTLSMIEEEIRAAQEREEELKRQRQVLQSTQSPRTKNAPSLPSRTCYKTAPGKIEKVKPPPSPTTEGPSLQPDLAPEEAAGTQRPKNLMQTLMEDYETHKSKRRERMDDSSVLEATRVNRRKSALALRWEAGIYANQEEEDNE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2D5 As Substrate

Site PTM Type Enzyme
S27 Phosphorylation
Y30 Phosphorylation
S31 Phosphorylation
S44 Phosphorylation
S91 Phosphorylation
S118 Phosphorylation
S121 Phosphorylation
S135 Phosphorylation
S137 Phosphorylation
S146 Phosphorylation
S152 Phosphorylation
S154 Phosphorylation
S155 Phosphorylation
T157 Phosphorylation
S158 Phosphorylation
S159 Phosphorylation
T169 Phosphorylation
T172 Phosphorylation
K174 Sumoylation
Y204 Phosphorylation
K208 Ubiquitination
S212 Phosphorylation
K220 Ubiquitination
S324 Phosphorylation
Y356 Phosphorylation
S361 Phosphorylation
S364 Phosphorylation
S375 Phosphorylation
S383 Phosphorylation
S393 Phosphorylation
T399 Phosphorylation
S401 Phosphorylation
S405 Phosphorylation
T409 Phosphorylation
S411 Phosphorylation
Y418 Phosphorylation
S419 Phosphorylation
S422 Phosphorylation
K429 Ubiquitination
K448 Ubiquitination
S461 Phosphorylation
S465 Phosphorylation
T467 Phosphorylation
S473 Phosphorylation
S479 Phosphorylation
S501 Phosphorylation
S517 Phosphorylation
S522 Phosphorylation
T526 Phosphorylation
Y550 Phosphorylation
Y568 Phosphorylation
S622 Phosphorylation
S630 Phosphorylation
S631 Phosphorylation
Y680 Phosphorylation
K683 Ubiquitination
Y684 Phosphorylation
K710 Ubiquitination
T718 Phosphorylation
S720 Phosphorylation
S745 Phosphorylation
T746 Phosphorylation
S748 Phosphorylation
S756 Phosphorylation
Y763 Phosphorylation
T765 Phosphorylation
S778 Phosphorylation
T780 Phosphorylation
T781 Phosphorylation
S785 Phosphorylation
T798 Phosphorylation
Y812 Phosphorylation
T814 Phosphorylation
S817 Phosphorylation
S839 Phosphorylation
Y850 Phosphorylation

Research Backgrounds

Function:

Binds to regulatory subunit (RII) of protein kinase A. May be involved in establishing polarity in signaling systems or in integrating PKA-RII isoforms with downstream effectors to capture, amplify and focus diffuse, trans-cellular signals carried by cAMP (By similarity).

Family&Domains:

The RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

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