MOBL3 Antibody | Affinity Biosciences

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Western blot analysis on rat brain cell lysates using MOBL3 Antibody,The lane on the left was treated with the antigen-specific peptide.

Product:	MOBL3 Antibody
Catalog:	AF0726
Description:	Rabbit polyclonal antibody to MOBL3
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Chicken, Xenopus
Mol.Wt.:	26kDa; 26kD(Calculated).
Uniprot:	Q9Y3A3
RRID:	AB_2834354

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(88%), Chicken(100%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

MOBL3 Antibody detects endogenous levels of total MOBL3.

RRID:

AB_2834354
Cite Format: Affinity Biosciences Cat# AF0726, RRID:AB_2834354.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2C4D; CGI 95; class II mMOB1; MGC12264; MOB family member 4, phocein; MOB like protein phocein; MOB1; mob1 homolog 3; MOB1, Mps One Binder kinase activator like 3; Mob3; MOB4; MOBKL3; MOBL3_HUMAN; mps one binder kinase activator like 3; Mps one binder kinase activator-like 3; Phocein Mob like protein; PHOCN; PREI3; Preimplantation protein 3; RCJMB04_1o21;

Immunogens

Immunogen:

Uniprot:

Q9Y3A3(PHOCN_HUMAN) >>Visit HPA database.

Gene(ID):

MOB4(25843)

Description:

This gene was identified based on its similarity with the mouse counterpart. Studies of the mouse counterpart suggest that the expression of this gene may be regulated during oocyte maturation and preimplantation following zygotic gene activation. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Sequence:

Q9Y3A3 PHOCN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVMAEGTAVLRRNRPGTKAQDFYNWPDESFDEMDSTLAVQQYIQQNIRADCSNIDKILEPPEGQDEGVWKYEHLRQFCLELNGLAVKLQSECHPDTCTQMTATEQWIFLCAAHKTPKECPAIDYTRHTLDGAACLLNSNKYFPSRVSIKESSVAKLGSVCRRIYRIFSHAYFHHRQIFDEYENETFLCHRFTKFVMKYNLMSKDNLIVPILEEEVQNSVSGESEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Chicken

100

Zebrafish

Xenopus

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y3A3 As Substrate

Q9Y3A3

Site	PTM Type	Source
C134	S-Nitrosylation	Uniprot
K140	Acetylation	Uniprot
K140	Ubiquitination	Uniprot
Y141	Phosphorylation	Uniprot
S147	Phosphorylation	Uniprot
K149	Ubiquitination	Uniprot
K155	Acetylation	Uniprot
T192	Phosphorylation	Uniprot
Y198	Phosphorylation	Uniprot

Research Backgrounds

Q9Y3A3_PHOCN_HUMAN

Function:

May play a role in membrane trafficking, specifically in membrane budding reactions.

PTMs:

Phosphorylated on serine residues.

Subcellular Location:

Cytoplasm>Perinuclear region. Membrane>Peripheral membrane protein. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein.
Note: In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks.

Subunit Structure:

Binds STRN4 (By similarity). Interacts with DNM1 and EPS15 (By similarity). Interacts with nucleoside diphosphate kinase (By similarity). Binds STRN and STRN3. Part of a ternary complex containing MOB4/PHOCN, STRN and/or STRN3 and PPA2. Interacts with CTTNBP2 (By similarity). Interacts with CTTNBP2NL.

Family&Domains:

Belongs to the MOB1/phocein family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MOBL3 Antibody - #AF0726