Product: PODN Antibody
Catalog: DF10168
Description: Rabbit polyclonal antibody to PODN
Application: WB
Reactivity: Human
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 69 kDa; 69kD(Calculated).
Uniprot: Q7Z5L7
RRID: AB_2840747

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PODN Antibody detects endogenous levels of total PODN.
RRID:
AB_2840747
Cite Format: Affinity Biosciences Cat# DF10168, RRID:AB_2840747.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

9430070G18; LRRGT00160; MGC24995; PCAN; Podn; PODN_HUMAN; Podocan; Podocan proteoglycan; RP23 40G2.7; SLRR5A;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q7Z5L7 PODN_HUMAN:

Kidney, heart, liver, pancreas and vascular smooth muscle cells. Also detected in aortic intima (at protein level).

Sequence:
MAQSRVLLLLLLLPPQLHLGPVLAVRAPGFGRSGGHSLSPEENEFAEEEPVLVLSPEEPGPGPAAVSCPRDCACSQEGVVDCGGIDLREFPGDLPEHTNHLSLQNNQLEKIYPEELSRLHRLETLNLQNNRLTSRGLPEKAFEHLTNLNYLYLANNKLTLAPRFLPNALISVDFAANYLTKIYGLTFGQKPNLRSVYLHNNKLADAGLPDNMFNGSSNVEVLILSSNFLRHVPKHLPPALYKLHLKNNKLEKIPPGAFSELSSLRELYLQNNYLTDEGLDNETFWKLSSLEYLDLSSNNLSRVPAGLPRSLVLLHLEKNAIRSVDANVLTPIRSLEYLLLHSNQLREQGIHPLAFQGLKRLHTVHLYNNALERVPSGLPRRVRTLMILHNQITGIGREDFATTYFLEELNLSYNRITSPQVHRDAFRKLRLLRSLDLSGNRLHTLPPGLPRNVHVLKVKRNELAALARGALVGMAQLRELYLTSNRLRSRALGPRAWVDLAHLQLLDIAGNQLTEIPEGLPESLEYLYLQNNKISAVPANAFDSTPNLKGIFLRFNKLAVGSVVDSAFRRLKHLQVLDIEGNLEFGDISKDRGRLGKEKEEEEEEEEEEEETR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
92
Xenopus
78
Chicken
67
Zebrafish
60
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z5L7 As Substrate

Site PTM Type Enzyme
T124 Phosphorylation
Y152 Phosphorylation
T159 Phosphorylation
S259 Phosphorylation
S263 Phosphorylation
N281 N-Glycosylation
N299 N-Glycosylation
S544 Phosphorylation
T545 Phosphorylation
S562 Phosphorylation

Research Backgrounds

Function:

Negatively regulates cell proliferation and cell migration.

PTMs:

N-glycosylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Cytoplasm.

Cytoplasm.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Kidney, heart, liver, pancreas and vascular smooth muscle cells. Also detected in aortic intima (at protein level).

Subunit Structure:

Binds to type I collagen.

Family&Domains:

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class V subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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