PODXL2 Antibody - #DF10171

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Product: PODXL2 Antibody
Catalog: DF10171
Description: Rabbit polyclonal antibody to PODXL2
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Rabbit, Dog
Mol.Wt.: 65 kDa; 65kD(Calculated).
Uniprot: Q9NZ53
RRID: AB_2840750

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PODXL2 Antibody detects endogenous levels of total PODXL2.
RRID:
AB_2840750
Cite Format: Affinity Biosciences Cat# DF10171, RRID:AB_2840750.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EG; OTTHUMP00000216064; PODLX2; podocalyxin like 2; Podocalyxin like protein 2; PODXL2;

Immunogens

Immunogen:
Uniprot:

Q9NZ53(PDXL2_HUMAN) >>Visit HPA database.

Gene(ID):
PODXL2(50512)
Expression:
Q9NZ53 PDXL2_HUMAN:

Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells.

Sequence:
MGRLLRAARLPPLLSPLLLLLVGGAFLGACVAGSDEPGPEGLTSTSLLDLLLPTGLEPLDSEEPSETMGLGAGLGAPGSGFPSEENEESRILQPPQYFWEEEEELNDSSLDLGPTADYVFPDLTEKAGSIEDTSQAQELPNLPSPLPKMNLVEPPWHMPPREEEEEEEEEEEREKEEVEKQEEEEEEELLPVNGSQEEAKPQVRDFSLTSSSQTPGATKSRHEDSGDQASSGVEVESSMGPSLLLPSVTPTTVTPGDQDSTSQEAEATVLPAAGLGVEFEAPQEASEEATAGAAGLSGQHEEVPALPSFPQTTAPSGAEHPDEDPLGSRTSASSPLAPGDMELTPSSATLGQEDLNQQLLEGQAAEAQSRIPWDSTQVICKDWSNLAGKNYIILNMTENIDCEVFRQHRGPQLLALVEEVLPRHGSGHHGAWHISLSKPSEKEQHLLMTLVGEQGVVPTQDVLSMLGDIRRSLEEIGIQNYSTTSSCQARASQVRSDYGTLFVVLVVIGAICIIIIALGLLYNCWQRRLPKLKHVSHGEELRFVENGCHDNPTLDVASDSQSEMQEKHPSLNGGGALNGPGSWGALMGGKRDPEDSDVFEEDTHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
88
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NZ53 As Substrate

Site PTM Type Enzyme
S134 O-Glycosylation
S144 O-Glycosylation
S144 Phosphorylation
S195 Phosphorylation
T209 O-Glycosylation
T209 Phosphorylation
S210 O-Glycosylation
S210 Phosphorylation
S211 O-Glycosylation
S212 O-Glycosylation
S212 Phosphorylation
T214 O-Glycosylation
T214 Phosphorylation
T218 O-Glycosylation
S220 Phosphorylation
S492 Phosphorylation
K533 Ubiquitination
S558 Phosphorylation
S560 Phosphorylation
S562 Phosphorylation
K567 Ubiquitination
S570 Phosphorylation
S582 Phosphorylation
S596 Phosphorylation

Research Backgrounds

Function:

Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins.

PTMs:

Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides.

Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells.

Subunit Structure:

Homodimer; disulfide-linked. Interacts with SELL, SELE and SELP.

Family&Domains:

Belongs to the podocalyxin family.

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