Product: STA13 Antibody
Catalog: AF0732
Description: Rabbit polyclonal antibody to STA13
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 125kDa; 125kD(Calculated).
Uniprot: Q9Y3M8
RRID: AB_2834356

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(91%), Dog(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
STA13 Antibody detects endogenous levels of total STA13.
RRID:
AB_2834356
Cite Format: Affinity Biosciences Cat# AF0732, RRID:AB_2834356.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

46H23.2; ARHGAP37; Deleted in liver cancer 2 protein; Deleted in liver cancer protein 2; DLC-2; DLC2; GT650; Rho GTPase activating protein on chromosome 13q12; Rho GTPase-activating protein; STA13; STA13_HUMAN; StAR related lipid transfer (START) domain containing 13; StAR-related lipid transfer protein 13; StARD13; START domain-containing protein 13;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y3M8 STA13_HUMAN:

Ubiquitously expressed. Underexpressed in hepatocellular carcinoma cells and some breast cancer cell lines.

Description:
StARD13 GTPase-activating protein for RhoA, and perhaps for Cdc42. May be involved in regulation of cytoskeletal reorganization, cell proliferation and cell motility. Acts a tumor suppressor in hepatocellular carcinoma cells. Homodimer. Interacts with TAX1BP1. 5 isoforms of the human protein are produced by alternative splicing.
Sequence:
MFSQVPRTPASGCYYLNSMTPEGQEMYLRFDQTTRRSPYRMSRILARHQLVTKIQQEIEAKEACDWLRAAGFPQYAQLYEDSQFPINIVAVKNDHDFLEKDLVEPLCRRLNTLNKCASMKLDVNFQRKKGDDSDEEDLCISNKWTFQRTSRRWSRVDDLYTLLPRGDRNGSPGGTGMRNTTSSESVLTDLSEPEVCSIHSESSGGSDSRSQPGQCCTDNPVMLDAPLVSSSLPQPPRDVLNHPFHPKNEKPTRARAKSFLKRMETLRGKGAHGRHKGSGRTGGLVISGPMLQQEPESFKAMQCIQIPNGDLQNSPPPACRKGLPCSGKSSGESSPSEHSSSGVSTPCLKERKCHEANKRGGMYLEDLDVLAGTALPDAGDQSRMHEFHSQENLVVHIPKDHKPGTFPKALSIESLSPTDSSNGVNWRTGSISLGREQVPGAREPRLMASCHRASRVSIYDNVPGSHLYASTGDLLDLEKDDLFPHLDDILQHVNGLQEVVDDWSKDVLPELQTHDTLVGEPGLSTFPSPNQITLDFEGNSVSEGRTTPSDVERDVTSLNESEPPGVRDRRDSGVGASLTRPNRRLRWNSFQLSHQPRPAPASPHISSQTASQLSLLQRFSLLRLTAIMEKHSMSNKHGWTWSVPKFMKRMKVPDYKDKAVFGVPLIVHVQRTGQPLPQSIQQALRYLRSNCLDQVGLFRKSGVKSRIHALRQMNENFPENVNYEDQSAYDVADMVKQFFRDLPEPLFTNKLSETFLHIYQYVSKEQRLQAVQAAILLLADENREVLQTLLCFLNDVVNLVEENQMTPMNLAVCLAPSLFHLNLLKKESSPRVIQKKYATGKPDQKDLNENLAAAQGLAHMIMECDRLFEVPHELVAQSRNSYVEAEIHVPTLEELGTQLEESGATFHTYLNHLIQGLQKEAKEKFKGWVTCSSTDNTDLAFKKVGDGNPLKLWKASVEVEAPPSVVLNRVLRERHLWDEDFVQWKVVETLDRQTEIYQYVLNSMAPHPSRDFVVLRTWKTDLPKGMCTLVSLSVEHEEAQLLGGVRAVVMDSQYLIEPCGSGKSRLTHICRIDLKGHSPEWYSKGFGHLCAAEVARIRNSFQPLIAEGPETKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
91
Rabbit
91
Xenopus
82
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y3M8 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
S37 Phosphorylation
Y39 Phosphorylation
S42 Phosphorylation
K100 Ubiquitination
S118 Phosphorylation
S133 Phosphorylation
S141 Phosphorylation
S154 Phosphorylation
Y160 Phosphorylation
T161 Phosphorylation
S171 Phosphorylation
K247 Ubiquitination
R267 Methylation
S314 Phosphorylation
S334 Phosphorylation
T345 Phosphorylation
S389 Phosphorylation
S411 Phosphorylation
S414 Phosphorylation
S416 Phosphorylation
S430 Phosphorylation
S454 Phosphorylation
S457 Phosphorylation
Y459 Phosphorylation
Y468 Phosphorylation
S470 Phosphorylation
T547 Phosphorylation
S572 Phosphorylation
S577 Phosphorylation
S589 Phosphorylation
S828 Phosphorylation
S829 Phosphorylation
T930 Phosphorylation
K942 Ubiquitination

Research Backgrounds

Function:

GTPase-activating protein for RhoA, and perhaps for Cdc42. May be involved in regulation of cytoskeletal reorganization, cell proliferation and cell motility. Acts a tumor suppressor in hepatocellular carcinoma cells.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein>Cytoplasmic side. Mitochondrion membrane>Peripheral membrane protein>Cytoplasmic side. Lipid droplet.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Underexpressed in hepatocellular carcinoma cells and some breast cancer cell lines.

Subunit Structure:

Homodimer. Interacts with TAX1BP1.

References

1). Ma C et al. Phillygenin inhibited M1 macrophage polarization and reduced hepatic stellate cell activation by inhibiting macrophage exosomal miR-125b-5p. BIOMEDICINE & PHARMACOTHERAPY 2023 Mar;159:114264. (PubMed: 36652738) [IF=7.5]

Application: WB    Species: Mouse    Sample: mHSCs

Fig. 5. PHI inhibited HSC activation through macrophage exosomal miR-125b-5p targeting Stard13. (a) The expression of miR-125b-5p, miR-21a-5p, miR-199a-5p, miR-29b-3p and miR-223–3p in RAW264.7 cells after treatment with LPS/IFNγ and PHI for 12 h was detected by RT-qPCR (n = 3). ###P 

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