Product: C3AR1 Antibody
Catalog: DF10198
Description: Rabbit polyclonal antibody to C3AR1
Application: WB
Reactivity: Human
Prediction: Rabbit
Mol.Wt.: 54 kDa; 54kD(Calculated).
Uniprot: Q16581
RRID: AB_2840777

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rabbit(83%)
Clonality:
Polyclonal
Specificity:
C3AR1 Antibody detects endogenous levels of total C3AR1.
RRID:
AB_2840777
Cite Format: Affinity Biosciences Cat# DF10198, RRID:AB_2840777.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AZ 3B; AZ3B; C3a anaphylatoxin chemotactic receptor; C3A R; C3A R1; C3a-R; C3AR; C3AR_HUMAN; C3AR1; C3R1; Complement component 3 receptor 1; Complement component 3a receptor 1; HNFAG09;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q16581 C3AR_HUMAN:

Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

Sequence:
MASFSAETNSTDLLSQPWNEPPVILSMVILSLTFLLGLPGNGLVLWVAGLKMQRTVNTIWFLHLTLADLLCCLSLPFSLAHLALQGQWPYGRFLCKLIPSIIVLNMFASVFLLTAISLDRCLVVFKPIWCQNHRNVGMACSICGCIWVVAFVMCIPVFVYREIFTTDNHNRCGYKFGLSSSLDYPDFYGDPLENRSLENIVQPPGEMNDRLDPSSFQTNDHPWTVPTVFQPQTFQRPSADSLPRGSARLTSQNLYSNVFKPADVVSPKIPSGFPIEDHETSPLDNSDAFLSTHLKLFPSASSNSFYESELPQGFQDYYNLGQFTDDDQVPTPLVAITITRLVVGFLLPSVIMIACYSFIVFRMQRGRFAKSQSKTFRVAVVVVAVFLVCWTPYHIFGVLSLLTDPETPLGKTLMSWDHVCIALASANSCFNPFLYALLGKDFRKKARQSIQGILEAAFSEELTRSTHCPSNNVISERNSTTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
83
Bovine
75
Horse
67
Sheep
67
Dog
50
Chicken
44
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16581 As Substrate

Site PTM Type Enzyme
S266 O-Glycosylation
S266 Phosphorylation
S349 Phosphorylation
S459 Phosphorylation
T463 Phosphorylation
S465 Phosphorylation
T466 Phosphorylation
S470 Phosphorylation
S475 Phosphorylation
S479 Phosphorylation
T480 Phosphorylation
T481 Phosphorylation

Research Backgrounds

Function:

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

PTMs:

Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.

O-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

Subunit Structure:

Interacts with VGF-derived peptide TLQP-21 (By similarity).

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

References

1). Aristolochic acid I promotes the invasion and migration of hepatocellular carcinoma cells by activating the C3a/C3aR complement system. TOXICOLOGY LETTERS (PubMed: 32898628) [IF=3.5]

Application: WB    Species: mouse    Sample: Hepa1-6 cells

Fig 4.| The eff ects of AAI exposure on C3a secretion and C3aR mRNA and protein expression in Hepa1-6 cells. Hepa1-6 cells were exposed to diff erent concentrations of AAI (0, 2.5, 5, or 10 μM) for 24 h. (A) Enzyme-linked immunosorbent assay was performed to measure AAI-induced excess C3a secretion (B) Western blot assays were performed to measure the expression of the C3aR protein.

Application: IF/ICC    Species: mouse    Sample: Hepa1-6 cells

Fig 4.| The eff ects of AAI exposure on C3a secretion and C3aR mRNA and protein expression in Hepa1-6 cells. Hepa1-6 cells were exposed to diff erent concentrations of AAI (0, 2.5, 5, or 10 μM) for 24 h. (D) Immunofluorescence images stained for C3aR (green) and DAPI (blue) Scale bar, 50 μm. Each bar represents the mean ±SD based on three replicates. P < 0.05 was considered to be statistically significant. ** compared with vehicle control (0 μM), P <0.01; ***compared with vehicle control (0 μM), P < 0.001; ****compared with vehicle control(0 μM), P < 0.0001

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