GPR125 Antibody | Affinity Biosciences

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GPR125 Antibody - Western blot analysis GPR125 using HUVEC whole cell lysates.

Product:	GPR125 Antibody
Catalog:	DF10218
Description:	Rabbit polyclonal antibody to GPR125
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	146 kDa; 146kD(Calculated).
Uniprot:	Q8IWK6
RRID:	AB_2840796

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

GPR125 Antibody detects endogenous levels of total GPR125.

RRID:

AB_2840796
Cite Format: Affinity Biosciences Cat# DF10218, RRID:AB_2840796.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ38547; G protein coupled receptor 125; PGR21; Probable G protein coupled receptor 125 precursor; TEM5 like; TEM5L;

Immunogens

Immunogen:

Uniprot:

Q8IWK6(AGRA3_HUMAN) >>Visit HPA database.

Gene(ID):

ADGRA3(166647)

Sequence:

Q8IWK6 AGRA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPPGRRRGRAQPPLLLPLSLLALLALLGGGGGGGAAALPAGCKHDGRPRGAGRAAGAAEGKVVCSSLELAQVLPPDTLPNRTVTLILSNNKISELKNGSFSGLSLLERLDLRNNLISSIDPGAFWGLSSLKRLDLTNNRIGCLNADIFRGLTNLVRLNLSGNLFSSLSQGTFDYLASLRSLEFQTEYLLCDCNILWMHRWVKEKNITVRDTRCVYPKSLQAQPVTGVKQELLTCDPPLELPSFYMTPSHRQVVFEGDSLPFQCMASYIDQDMQVLWYQDGRIVETDESQGIFVEKNMIHNCSLIASALTISNIQAGSTGNWGCHVQTKRGNNTRTVDIVVLESSAQYCPPERVVNNKGDFRWPRTLAGITAYLQCTRNTHGSGIYPGNPQDERKAWRRCDRGGFWADDDYSRCQYANDVTRVLYMFNQMPLNLTNAVATARQLLAYTVEAANFSDKMDVIFVAEMIEKFGRFTKEEKSKELGDVMVDIASNIMLADERVLWLAQREAKACSRIVQCLQRIATYRLAGGAHVYSTYSPNIALEAYVIKSTGFTGMTCTVFQKVAASDRTGLSDYGRRDPEGNLDKQLSFKCNVSNTFSSLALKNTIVEASIQLPPSLFSPKQKRELRPTDDSLYKLQLIAFRNGKLFPATGNSTNLADDGKRRTVVTPVILTKIDGVNVDTHHIPVNVTLRRIAHGADAVAARWDFDLLNGQGGWKSDGCHILYSDENITTIQCYSLSNYAVLMDLTGSELYTQAASLLHPVVYTTAIILLLCLLAVIVSYIYHHSLIRISLKSWHMLVNLCFHIFLTCVVFVGGITQTRNASICQAVGIILHYSTLATVLWVGVTARNIYKQVTKKAKRCQDPDEPPPPPRPMLRFYLIGGGIPIIVCGITAAANIKNYGSRPNAPYCWMAWEPSLGAFYGPASFITFVNCMYFLSIFIQLKRHPERKYELKEPTEEQQRLAANENGEINHQDSMSLSLISTSALENEHTFHSQLLGASLTLLLYVALWMFGALAVSLYYPLDLVFSFVFGATSLSFSAFFVVHHCVNREDVRLAWIMTCCPGRSSYSVQVNVQPPNSNGTNGEAPKCPNSSAESSCTNKSASSFKNSSQGCKLTNLQAAAAQCHANSLPLNSTPQLDNSLTEHSMDNDIKMHVAPLEVQFRTNVHSSRHHKNRSKGHRASRLTVLREYAYDVPTSVEGSVQNGLPKSRLGNNEGHSRSRRAYLAYRERQYNPPQQDSSDACSTLPKSSRNFEKPVSTTSKKDALRKPAVVELENQQKSYGLNLAIQNGPIKSNGQEGPLLGTDSTGNVRTGLWKHETTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Pig

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IWK6 As Substrate

Q8IWK6

Site	PTM Type	Source
T208	Phosphorylation	Uniprot
T334	Phosphorylation	Uniprot
T336	Phosphorylation	Uniprot
S344	Phosphorylation	Uniprot
S632	Phosphorylation	Uniprot
K635	Ubiquitination	Uniprot
Y878	Phosphorylation	Uniprot
Y950	Phosphorylation	Uniprot
S1097	Phosphorylation	Uniprot
T1099	Phosphorylation	Uniprot
S1102	Phosphorylation	Uniprot
Y1224	Phosphorylation	Uniprot
S1240	Phosphorylation	Uniprot
K1248	Ubiquitination	Uniprot
S1250	Phosphorylation	Uniprot
K1316	Ubiquitination	Uniprot

Research Backgrounds

Q8IWK6_AGRA3_HUMAN

Function:

Orphan receptor that may have a role in planar cell polarity pathway.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts (via PDZ-binding motif) with DLG1.

Family&Domains:

Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.

Restrictive clause

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For Research Use Only.
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GPR125 Antibody - #DF10218