SUPT16H Antibody | Affinity Biosciences

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Western blot analysis on HepG2 cell lysates using SUPT16H Antibody,The lane on the left was treated with the antigen-specific peptide.

AF0822 at 1/100 staining rat testis tissue by IHC-P.

Product:	SUPT16H Antibody
Catalog:	AF0822
Description:	Rabbit polyclonal antibody to SUPT16H
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	119kDa; 120kD(Calculated).
Uniprot:	Q9Y5B9
RRID:	AB_2834361

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SUPT16H Antibody detects endogenous levels of total SUPT16H.

RRID:

AB_2834361
Cite Format: Affinity Biosciences Cat# AF0822, RRID:AB_2834361.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CDC68; Chromatin specific transcription elongation factor 140 kDa subunit; Chromatin-specific transcription elongation factor 140 kDa subunit; Facilitates chromatin transcription complex subunit SPT16; FACT 140 kDa subunit; FACT; FACT complex subunit SPT16; FACTp140; FLJ10857; FLJ14010; hSPT16; SP16H_HUMAN; Suppressor of Ty 16 homolog; Supt16h;

Immunogens

Immunogen:

Uniprot:

Q9Y5B9(SP16H_HUMAN) >>Visit HPA database.

Gene(ID):

SUPT16H(11198)

Expression:

Q9Y5B9 SP16H_HUMAN:

Ubiquitous.

Description:

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene.

Sequence:

Q9Y5B9 SP16H_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVTLDKDAYYRRVKRLYSNWRKGEDEYANVDAIVVSVGVDEEIVYAKSTALQTWLFGYELTDTIMVFCDDKIIFMASKKKVEFLKQIANTKGNENANGAPAITLLIREKNESNKSSFDKMIEAIKESKNGKKIGVFSKDKFPGEFMKSWNDCLNKEGFDKIDISAVVAYTIAVKEDGELNLMKKAASITSEVFNKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFKSYCSNLVRTLMVDPSQEVQENYNFLLQLQEELLKELRHGVKICDVYNAVMDVVKKQKPELLNKITKNLGFGMGIEFREGSLVINSKNQYKLKKGMVFSINLGFSDLTNKEGKKPEEKTYALFIGDTVLVDEDGPATVLTSVKKKVKNVGIFLKNEDEEEEEEEKDEAEDLLGRGSRAALLTERTRNEMTAEEKRRAHQKELAAQLNEEAKRRLTEQKGEQQIQKARKSNVSYKNPSLMPKEPHIREMKIYIDKKYETVIMPVFGIATPFHIATIKNISMSVEGDYTYLRINFYCPGSALGRNEGNIFPNPEATFVKEITYRASNIKAPGEQTVPALNLQNAFRIIKEVQKRYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDILYNNIKHALFQPCDGEMIIVLHFHLKNAIMFGKKRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMEREMRHKLKTAFKNFIEKVEALTKEELEFEVPFRDLGFNGAPYRSTCLLQPTSSALVNATEWPPFVVTLDEVELIHFERVQFHLKNFDMVIVYKDYSKKVTMINAIPVASLDPIKEWLNSCDLKYTEGVQSLNWTKIMKTIVDDPEGFFEQGGWSFLEPEGEGSDAEEGDSESEIEDETFNPSEDDYEEEEEDSDEDYSSEAEESDYSKESLGSEEESGKDWDELEEEARKADRESRYEEEEEQSRSMSRKRKASVHSSGRGSNRGSRHSSAPPKKKRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5B9 As Substrate

Q9Y5B9

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
K7	Acetylation		Uniprot
K7	Ubiquitination		Uniprot
R12	Methylation		Uniprot
R13	Methylation		Uniprot
S19	Phosphorylation		Uniprot
S78	Phosphorylation		Uniprot
K80	Ubiquitination		Uniprot
K86	Ubiquitination		Uniprot
K92	Ubiquitination		Uniprot
K120	Acetylation		Uniprot
K120	Ubiquitination		Uniprot
S138	Phosphorylation		Uniprot
K139	Acetylation		Uniprot
K139	Ubiquitination		Uniprot
K156	Acetylation		Uniprot
K156	Methylation		Uniprot
K156	Ubiquitination		Uniprot
K185	Ubiquitination		Uniprot
S188	Phosphorylation		Uniprot
S191	Phosphorylation		Uniprot
K196	Acetylation		Uniprot
K196	Ubiquitination		Uniprot
K199	Ubiquitination		Uniprot
K211	Acetylation		Uniprot
K211	Ubiquitination		Uniprot
K216	Acetylation		Uniprot
K216	Ubiquitination		Uniprot
K223	Acetylation		Uniprot
K223	Ubiquitination		Uniprot
K263	Ubiquitination		Uniprot
K280	Acetylation		Uniprot
K280	Methylation		Uniprot
K280	Ubiquitination		Uniprot
Y326	Phosphorylation		Uniprot
K335	Ubiquitination		Uniprot
S360	Phosphorylation		Uniprot
K366	Ubiquitination		Uniprot
K392	Acetylation		Uniprot
K426	Ubiquitination		Uniprot
K433	Sumoylation		Uniprot
K433	Ubiquitination		Uniprot
K444	Ubiquitination		Uniprot
S455	Phosphorylation		Uniprot
K479	Sumoylation		Uniprot
K479	Ubiquitination		Uniprot
K490	Ubiquitination		Uniprot
T494	Phosphorylation	Q96GD4 (AURKB)	Uniprot
K497	Sumoylation		Uniprot
K497	Ubiquitination		Uniprot
K504	Ubiquitination		Uniprot
S508	Phosphorylation		Uniprot
K513	Acetylation		Uniprot
K513	Ubiquitination		Uniprot
K520	Ubiquitination		Uniprot
K528	Acetylation		Uniprot
Y535	Phosphorylation		Uniprot
T547	Phosphorylation		Uniprot
S558	Phosphorylation		Uniprot
Y565	Phosphorylation		Uniprot
T566	Phosphorylation		Uniprot
Y567	Phosphorylation		Uniprot
T593	Phosphorylation		Uniprot
K596	Acetylation		Uniprot
K596	Ubiquitination		Uniprot
T599	Phosphorylation		Uniprot
Y600	Phosphorylation		Uniprot
S603	Phosphorylation		Uniprot
K606	Ubiquitination		Uniprot
K626	Acetylation		Uniprot
K626	Ubiquitination		Uniprot
T634	Phosphorylation		Uniprot
K640	Ubiquitination		Uniprot
K647	Sumoylation		Uniprot
K647	Ubiquitination		Uniprot
S650	Phosphorylation		Uniprot
S658	Phosphorylation		Uniprot
K663	Acetylation		Uniprot
K663	Sumoylation		Uniprot
K663	Ubiquitination		Uniprot
K674	Acetylation		Uniprot
K674	Ubiquitination		Uniprot
K696	Ubiquitination		Uniprot
K732	Acetylation		Uniprot
K732	Ubiquitination		Uniprot
Y741	Phosphorylation		Uniprot
K753	Ubiquitination		Uniprot
R760	Methylation		Uniprot
Y764	Phosphorylation		Uniprot
K777	Ubiquitination		Uniprot
K781	Acetylation		Uniprot
K781	Ubiquitination		Uniprot
K786	Acetylation		Uniprot
K786	Ubiquitination		Uniprot
K792	Ubiquitination		Uniprot
Y861	Phosphorylation		Uniprot
Y864	Phosphorylation		Uniprot
S865	Phosphorylation		Uniprot
K867	Ubiquitination		Uniprot
K883	Ubiquitination		Uniprot
K892	Ubiquitination		Uniprot
T903	Phosphorylation		Uniprot
K904	Acetylation		Uniprot
K904	Ubiquitination		Uniprot
K907	Ubiquitination		Uniprot
S979	Phosphorylation		Uniprot
S982	Phosphorylation		Uniprot
S986	Phosphorylation		Uniprot
S1004	Phosphorylation		Uniprot
Y1006	Phosphorylation		Uniprot
S1013	Phosphorylation		Uniprot
R1014	Methylation		Uniprot
S1015	Phosphorylation		Uniprot
S1017	Phosphorylation		Uniprot
S1023	Phosphorylation		Uniprot
S1026	Phosphorylation		Uniprot
S1027	Phosphorylation		Uniprot
R1029	Methylation		Uniprot
S1031	Phosphorylation		Uniprot
R1033	Methylation		Uniprot
R1036	Methylation		Uniprot
S1038	Phosphorylation		Uniprot
S1039	Phosphorylation		Uniprot

Research Backgrounds

Q9Y5B9_SP16H_HUMAN

Function:

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).

PTMs:

ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.

Subcellular Location:

Nucleus. Chromosome.
Note: Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with MYOG (via C-terminal region) (By similarity). Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Interacts with NEK9. Binds to histone H2A-H2B. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1. Interacts with GTF2E2.

(Microbial infection) Interacts with Herpes simplex virus 1 (HHV-1) protein ICP22; this interaction relocalizes the FACT complex to viral genomes in infected cells.

Family&Domains:

The C-terminal Glu-rich acidic region is essential for FACT activity.

Belongs to the peptidase M24 family. SPT16 subfamily.

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SUPT16H Antibody - #AF0822