FUBP1 Antibody - #DF2924

(3)  
Product: FUBP1 Antibody
Catalog: DF2924
Description: Rabbit polyclonal antibody to FUBP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 74kDa; 68kD(Calculated).
Uniprot: Q96AE4
RRID: AB_2840910

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FUBP1 Antibody detects endogenous levels of total FUBP1.
RRID:
AB_2840910
Cite Format: Affinity Biosciences Cat# DF2924, RRID:AB_2840910.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA helicase V; far upstream element (FUSE) binding protein 1; Far upstream element (FUSE) binding protein 4; Far upstream element binding protein 1; far upstream element binding protein; Far upstream element-binding protein 1; FBP; FUBP; Fubp1; FUBP1_HUMAN; Fubp4; FUSE binding protein 1; FUSE-binding protein 1; HDH V;

Immunogens

Immunogen:
Uniprot:

Q96AE4(FUBP1_HUMAN) >>Visit HPA database.

Gene(ID):
FUBP1(8880)
Sequence:
MADYSTVPPPSSGSAGGGGGGGGGGGVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPDAKKVAPQNDSFGTQLPPMHQQQSRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDSGGLPERSCMLTGTPESVQSAKRLLDQIVEKGRPAPGFHHGDGPGNAVQEIMIPASKAGLVIGKGGETIKQLQERAGVKMVMIQDGPQNTGADKPLRITGDPYKVQQAKEMVLELIRDQGGFREVRNEYGSRIGGNEGIDVPIPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGTTPERIAQITGPPDRCQHAAEIITDLLRSVQAGNPGGPGPGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRNPPPNADPNMKLFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPGPAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGTDPNSAAWAAYYAHYYQQQAQPPPAAPAGAPTTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAPTGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQGMPQHPPAPQGQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96AE4 As Substrate

Site PTM Type Enzyme
A2 Acetylation
Y4 Phosphorylation
K32 Ubiquitination
K44 Acetylation
K44 Ubiquitination
S52 Phosphorylation
S55 Phosphorylation
Y58 Phosphorylation P12931 (SRC)
Y60 Phosphorylation
K64 Ubiquitination
K76 Acetylation
K76 Ubiquitination
K77 Acetylation
K77 Ubiquitination
S84 Phosphorylation
S99 Phosphorylation
Y105 Phosphorylation
R118 Methylation
K133 Ubiquitination
S140 Phosphorylation
S147 Phosphorylation
C148 S-Nitrosylation
T153 Phosphorylation
S156 Phosphorylation
S159 Phosphorylation
K161 Ubiquitination
K203 Ubiquitination
T207 Phosphorylation
K209 Ubiquitination
K218 Ubiquitination
T229 Phosphorylation
Y242 Phosphorylation
K243 Acetylation
K243 Ubiquitination
Y268 Phosphorylation P12931 (SRC)
S270 Phosphorylation
K300 Ubiquitination
R308 Methylation
K312 Acetylation
K312 Sumoylation
K312 Ubiquitination
R321 Methylation
R331 Methylation
R359 Methylation
R361 Methylation
R363 Methylation
K394 Ubiquitination
T398 Phosphorylation
K400 Ubiquitination
K425 Ubiquitination
R430 Methylation
T432 Phosphorylation
Y438 Phosphorylation
Y589 Phosphorylation
Y590 Phosphorylation
K591 Acetylation
K592 Ubiquitination
Y618 Phosphorylation
Y619 Phosphorylation
Y625 Phosphorylation P12931 (SRC)
Y626 Phosphorylation
T629 Phosphorylation
S630 Phosphorylation

Research Backgrounds

Function:

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.

PTMs:

Ubiquitinated. This targets the protein for proteasome-mediated degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Found in a complex with PUF60 and far upstream element (FUSE) DNA segment. Interacts with PUF60 and JTV1.

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