RNF20 Antibody - #DF2960
| Product: | RNF20 Antibody |
| Catalog: | DF2960 |
| Description: | Rabbit polyclonal antibody to RNF20 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Rabbit, Dog, Chicken |
| Mol.Wt.: | 114kDa; 114kD(Calculated). |
| Uniprot: | Q5VTR2 |
| RRID: | AB_2840943 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2960, RRID:AB_2840943.
Fold/Unfold
BRE1 A; BRE1; BRE1 E3 ubiquitin ligase homolog; BRE1-A; BRE1A; BRE1A_HUMAN; E3 ubiquitin-protein ligase BRE1A; hBRE1; Homolog of S. cerevisiae BRE1; RING finger protein 20; Ring finger protein 20 E3 ubiquitin protein ligase; Rnf20;
Immunogens
- Q5VTR2 BRE1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQDFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASKASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q5VTR2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| T122 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K194 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| Y314 | Phosphorylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| K324 | Sumoylation | Uniprot | |
| K348 | Acetylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| T444 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S548 | Phosphorylation | Uniprot | |
| S550 | Phosphorylation | Uniprot | |
| S553 | Phosphorylation | Uniprot | |
| K610 | Methylation | Uniprot | |
| K614 | Methylation | Uniprot | |
| Y650 | Phosphorylation | Uniprot | |
| K716 | Ubiquitination | Uniprot | |
| K762 | Ubiquitination | Uniprot | |
| K769 | Methylation | Uniprot | |
| K775 | Ubiquitination | Uniprot | |
| K778 | Sumoylation | Uniprot | |
| K808 | Acetylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| K821 | Ubiquitination | Uniprot | |
| K857 | Ubiquitination | Uniprot | |
| K872 | Ubiquitination | Uniprot | |
| K874 | Acetylation | Uniprot | |
| K880 | Acetylation | Uniprot | |
| K880 | Methylation | Uniprot | |
| K929 | Ubiquitination | Uniprot | |
| K930 | Ubiquitination | Uniprot |
Research Backgrounds
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Nucleus.
Expressed in the normal brain and also in malignant gliomas (at protein level).
Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Interacts with isoform 1 and isoform 2 of PA2G4.
Belongs to the BRE1 family.
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