SATB2 Antibody - #DF2962

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Product: SATB2 Antibody
Catalog: DF2962
Description: Rabbit polyclonal antibody to SATB2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 81kDa; 83kD(Calculated).
Uniprot: Q9UPW6
RRID: AB_2840944

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
SATB2 Antibody detects endogenous levels of total SATB2.
RRID:
AB_2840944
Cite Format: Affinity Biosciences Cat# DF2962, RRID:AB_2840944.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA binding protein SATB2; DNA-binding protein SATB2; FLJ21474; FLJ32076; GLSS; KIAA1034; MGC119474; MGC119477; SATB family member 2; SATB homeobox 2; SATB2; SATB2_HUMAN; Special AT rich sequence binding protein 2; Special AT-rich sequence-binding protein 2;

Immunogens

Immunogen:
Uniprot:

Q9UPW6(SATB2_HUMAN) >>Visit HPA database.

Gene(ID):
SATB2(23314)
Expression:
Q9UPW6 SATB2_HUMAN:

High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.

Sequence:
MERRSESPCLRDSPDRRSGSPDVKGPPPVKVARLEQNGSPMGARGRPNGAVAKAVGGLMIPVFCVVEQLDGSLEYDNREEHAEFVLVRKDVLFSQLVETALLALGYSHSSAAQAQGIIKLGRWNPLPLSYVTDAPDATVADMLQDVYHVVTLKIQLQSCSKLEDLPAEQWNHATVRNALKELLKEMNQSTLAKECPLSQSMISSIVNSTYYANVSATKCQEFGRWYKKYKKIKVERVERENLSDYCVLGQRPMHLPNMNQLASLGKTNEQSPHSQIHHSTPIRNQVPALQPIMSPGLLSPQLSPQLVRQQIAMAHLINQQIAVSRLLAHQHPQAINQQFLNHPPIPRAVKPEPTNSSVEVSPDIYQQVRDELKRASVSQAVFARVAFNRTQGLLSEILRKEEDPRTASQSLLVNLRAMQNFLNLPEVERDRIYQDERERSMNPNVSMVSSASSSPSSSRTPQAKTSTPTTDLPIKVDGANINITAAIYDEIQQEMKRAKVSQALFAKVAANKSQGWLCELLRWKENPSPENRTLWENLCTIRRFLNLPQHERDVIYEEESRHHHSERMQHVVQLPPEPVQVLHRQQSQPAKESSPPREEAPPPPPPTEDSCAKKPRSRTKISLEALGILQSFIHDVGLYPDQEAIHTLSAQLDLPKHTIIKFFQNQRYHVKHHGKLKEHLGSAVDVAEYKDEELLTESEENDSEEGSEEMYKVEAEEENADKSKAAPAEIDQR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
92
Xenopus
69
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPW6 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
S7 Phosphorylation
S13 Phosphorylation
S18 Phosphorylation
S20 Phosphorylation
S39 Phosphorylation
K184 Ubiquitination
K233 Sumoylation
S294 Phosphorylation
S303 Phosphorylation
R347 Methylation
K350 Sumoylation
S446 Phosphorylation
S449 Phosphorylation
S450 Phosphorylation
S452 Phosphorylation
S453 Phosphorylation
S454 Phosphorylation
T467 Phosphorylation
Y556 Phosphorylation
S587 Phosphorylation
S593 Phosphorylation
S594 Phosphorylation
S682 Phosphorylation

Research Backgrounds

Function:

Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

PTMs:

Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.

Subcellular Location:

Nucleus matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.

Subunit Structure:

Interacts with ATF4 and RUNX2; resulting in enhanced DNA binding and transactivation by these transcription factors (By similarity). Interacts with PIAS1.

Family&Domains:

Belongs to the CUT homeobox family.

References

1). Transcription Factor SATB2 Regulates Skeletal Muscle Cell Proliferation and Migration via HDAC4 in Pigs. Genes, 2024 (PubMed: 38254955) [IF=3.5]
2). Cis-Regulatory Evolution of CCNB1IP1 Driving Gradual Increase of Cortical Size and Folding in primates. bioRxiv : the preprint server for biology, 2024 (PubMed: 39713381)

Application: IF/ICC    Species: Mouse    Sample:

Fig.5. CCNB1IP1 conditional knock-in mice exhibit cortical expansion and folding pattern. (A)Western blotting analysis showing CCNB1IP1 upregulation in the P0 cortex of CCNB1IP1Nestin-Cre KI mice. (B) Dorsal view of WT and CCNB1IP1Nestin-Cre cKI P0 brains from the 2 nests. (ctrl n=7 mice, cKI n=8 mice, data represented as mean, *p < 0.05, unpaired Student’s t-test). (C)Cortical layer marker analysis revealed a significant increase in BRN2+(L2–4) but no significant changes in TBR1+(L6) in cKI. (ctrl n=7 mice, cKI n=8 mice, data represented as mean, *p < 0.05, unpaired Student’s t-test). (D)Representative images of cortical folding (pink arrows) in cKI Nestin-cre mice at P0. (E)Neuronal immunostaining showing a cortical folding pattern (arrowheads) in Cki. (F)and comparison of local gyrification indices in cKI. (ctrl n=6 mice, cKI n=6 mice, data represented as mean, ***p < 0.001, unpaired Student’s t-test). (G)Representative images of the cortical layer marker SATB2 (L2–4) and CTIP2 (L5) in cKI mice at P0. Area with blue rectangle to be magnified to show gyri and adjacent sulci structure with white rectangle in folding locations. (H)Quantification of STAB2 and CTIP2 between the gyri and adjacent sulci structure in both cKI and control mice. (ctrl n=6 mice, cKI n=6 mice, data represented as mean, *p < 0.05, unpaired Student’s t-test). (I)The percentages of cortical folding from 3 independent nests at P1 age.

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