CDC7 Antibody | Affinity Biosciences

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CDC7 Antibody - Western blot analysis of extracts from various samples, using CDC7 Antibody.

Product:	CDC7 Antibody
Catalog:	DF3005
Description:	Rabbit polyclonal antibody to CDC7
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	70 KD; 64kD(Calculated).
Uniprot:	O00311
RRID:	AB_2840984

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(91%), Bovine(82%), Sheep(90%), Rabbit(91%), Dog(91%), Chicken(89%)

Clonality:

Polyclonal

Specificity:

CDC7 Antibody detects endogenous levels of total CDC7.

RRID:

AB_2840984
Cite Format: Affinity Biosciences Cat# DF3005, RRID:AB_2840984.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cdc 7; CDC7; CDC7 cell division cycle 7; CDC7 cell division cycle 7 like 1; CDC7 L1; Cdc7 like 1; CDC7 related kinase; CDC7-related kinase; CDC7_HUMAN; CDC7L 1; CDC7L1; Cell division cycle 7 homolog; Cell division cycle 7 kinase; Cell division cycle 7 like protein 1; Cell division cycle 7 related protein kinase; Cell division cycle 7-related protein kinase; HsCDC 7; HsCdc7; Hsk 1; Hsk1; HuCDC 7; huCdc7; MGC117361; MGC126237; MGC126238;

Immunogens

Immunogen:

A synthesized peptide derived from human CDC7, corresponding to a region within N-terminal amino acids.

Uniprot:

O00311(CDC7_HUMAN) >>Visit HPA database.

Gene(ID):

CDC7(8317)

Sequence:

O00311 CDC7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEASLGIQMDEPMAFSPQRDRFQAEGSLKKNEQNFKLAGVKKDIEKLYEAVPQLSNVFKIEDKIGEGTFSSVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGVKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMLNLFKALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIELLKFVQSEAQQERCSQNKSHIITGNKIPLSGPVPKELDQQSTTKASVKRPYTNAQIQIKQGKDGKEGSVGLSVQRSVFGERNFNIHSSISHESPAVKLMKQSKTVDVLSRKLATKKKAISTKVMNSAVMRKTASSCPASLTCDCYATDKVCSICLSRRQQVAPRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQGHASHQPAISEKTDHKASCLVQTPPGQYSGNSFKKGDSNSCEHCFDEYNTNLEGWNEVPDEAYDLLDKLLDLNPASRITAEEALLHPFFKDMSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Rabbit

Sheep

Chicken

Bovine

Horse

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O00311_CDC7_HUMAN

Function:

Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.

Subcellular Location:

Nucleus.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CDC7 Antibody - #DF3005