TRPM7 Antibody - #DF7513

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Product: TRPM7 Antibody
Catalog: DF7513
Description: Rabbit polyclonal antibody to TRPM7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 213 KD; 213kD(Calculated).
Uniprot: Q96QT4
RRID: AB_2841012

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
TRPM7 Antibody detects endogenous levels of total TRPM7.
RRID:
AB_2841012
Cite Format: Affinity Biosciences Cat# DF7513, RRID:AB_2841012.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; Transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRPM7; TRPM7_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q96QT4(TRPM7_HUMAN) >>Visit HPA database.

Gene(ID):
TRPM7(54822)
Sequence:
MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGDHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKAEMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLPITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAGKVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFHSGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDPQCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNKNQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRVSSLAGFTDCHRTSIPVHSKQAEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGLTSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Horse
88
Bovine
88
Sheep
88
Dog
88
Xenopus
75
Chicken
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96QT4 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
T12 Phosphorylation
S57 Phosphorylation
K66 Ubiquitination
S101 Phosphorylation
K189 Ubiquitination
S233 Phosphorylation
K351 Ubiquitination
T523 Phosphorylation
S554 Phosphorylation
T555 Phosphorylation
K667 Ubiquitination
S764 Phosphorylation
Y776 Phosphorylation
T778 Phosphorylation
K827 Ubiquitination
S907 Phosphorylation
K1159 Ubiquitination
T1163 Phosphorylation Q96QT4 (TRPM7)
K1168 Acetylation
S1191 Phosphorylation Q96QT4 (TRPM7)
S1193 Phosphorylation Q96QT4 (TRPM7)
S1230 Phosphorylation
S1239 Phosphorylation
K1253 Ubiquitination
S1255 Phosphorylation Q96QT4 (TRPM7)
S1258 Phosphorylation Q96QT4 (TRPM7)
T1265 Phosphorylation Q96QT4 (TRPM7)
S1269 Phosphorylation
S1271 Phosphorylation Q96QT4 (TRPM7)
S1287 Phosphorylation Q96QT4 (TRPM7)
S1358 Phosphorylation Q96QT4 (TRPM7)
S1361 Phosphorylation
S1386 Phosphorylation
S1387 Phosphorylation Q96QT4 (TRPM7)
S1390 Phosphorylation Q96QT4 (TRPM7)
S1395 Phosphorylation
S1396 Phosphorylation
S1404 Phosphorylation Q96QT4 (TRPM7)
T1405 Phosphorylation Q96QT4 (TRPM7)
S1407 Phosphorylation Q96QT4 (TRPM7)
S1410 Phosphorylation
S1413 Phosphorylation
T1417 Phosphorylation
T1419 Phosphorylation
T1435 Phosphorylation Q96QT4 (TRPM7)
S1446 Phosphorylation Q96QT4 (TRPM7)
T1455 Phosphorylation Q96QT4 (TRPM7)
S1456 Phosphorylation Q96QT4 (TRPM7)
S1463 Phosphorylation Q96QT4 (TRPM7)
S1468 Phosphorylation Q96QT4 (TRPM7)
T1471 Phosphorylation Q96QT4 (TRPM7)
K1473 Methylation
K1473 Ubiquitination
S1476 Phosphorylation Q96QT4 (TRPM7)
S1477 Phosphorylation Q96QT4 (TRPM7)
T1482 Phosphorylation Q96QT4 (TRPM7)
S1488 Phosphorylation
S1493 Phosphorylation Q96QT4 (TRPM7)
S1500 Phosphorylation
S1504 Phosphorylation
T1505 Phosphorylation Q96QT4 (TRPM7)
T1508 Phosphorylation Q96QT4 (TRPM7)
S1513 Phosphorylation Q96QT4 (TRPM7)
S1527 Phosphorylation Q96QT4 (TRPM7)
S1533 Phosphorylation Q96QT4 (TRPM7)
T1537 Phosphorylation Q96QT4 (TRPM7)
T1542 Phosphorylation Q96QT4 (TRPM7)
S1543 Phosphorylation Q96QT4 (TRPM7)
T1551 Phosphorylation Q96QT4 (TRPM7)
S1567 Phosphorylation Q96QT4 (TRPM7)
S1569 Phosphorylation Q96QT4 (TRPM7)
T1583 Phosphorylation Q96QT4 (TRPM7)
S1598 Phosphorylation Q96QT4 (TRPM7)
S1615 Phosphorylation Q96QT4 (TRPM7)
S1660 Phosphorylation Q96QT4 (TRPM7)
T1685 Phosphorylation Q96QT4 (TRPM7)
S1695 Phosphorylation Q96QT4 (TRPM7)
S1779 Phosphorylation Q96QT4 (TRPM7)
T1830 Phosphorylation Q96QT4 (TRPM7)
K1833 Ubiquitination
S1851 Phosphorylation Q96QT4 (TRPM7)
S1860 Phosphorylation Q96QT4 (TRPM7)

PTMs - Q96QT4 As Enzyme

Substrate Site Source
O00418 (EEF2K) S78 Uniprot
P04083 (ANXA1) S5 Uniprot
P28289 (TMOD1) S2 Uniprot
P28289 (TMOD1) T54 Uniprot
P35579 (MYH9) T1800 Uniprot
P35579 (MYH9) S1803 Uniprot
P35579 (MYH9) S1808 Uniprot
Q96QT4 (TRPM7) T1163 Uniprot
Q96QT4 (TRPM7) S1191 Uniprot
Q96QT4 (TRPM7) S1193 Uniprot
Q96QT4 (TRPM7) S1255 Uniprot
Q96QT4 (TRPM7) S1258 Uniprot
Q96QT4 (TRPM7) T1265 Uniprot
Q96QT4 (TRPM7) S1271 Uniprot
Q96QT4 (TRPM7) S1287 Uniprot
Q96QT4 (TRPM7) S1358 Uniprot
Q96QT4 (TRPM7) S1387 Uniprot
Q96QT4 (TRPM7) S1390 Uniprot
Q96QT4 (TRPM7) S1404 Uniprot
Q96QT4 (TRPM7) T1405 Uniprot
Q96QT4 (TRPM7) S1407 Uniprot
Q96QT4 (TRPM7) T1435 Uniprot
Q96QT4 (TRPM7) S1446 Uniprot
Q96QT4 (TRPM7) T1455 Uniprot
Q96QT4 (TRPM7) S1456 Uniprot
Q96QT4 (TRPM7) S1463 Uniprot
Q96QT4 (TRPM7) S1468 Uniprot
Q96QT4 (TRPM7) T1471 Uniprot
Q96QT4 (TRPM7) S1476 Uniprot
Q96QT4 (TRPM7) S1477 Uniprot
Q96QT4 (TRPM7) T1482 Uniprot
Q96QT4 (TRPM7) S1493 Uniprot
Q96QT4 (TRPM7) T1505 Uniprot
Q96QT4 (TRPM7) T1508 Uniprot
Q96QT4 (TRPM7) S1513 Uniprot
Q96QT4 (TRPM7) S1527 Uniprot
Q96QT4 (TRPM7) S1533 Uniprot
Q96QT4 (TRPM7) T1537 Uniprot
Q96QT4 (TRPM7) T1542 Uniprot
Q96QT4 (TRPM7) S1543 Uniprot
Q96QT4 (TRPM7) T1551 Uniprot
Q96QT4 (TRPM7) S1567 Uniprot
Q96QT4 (TRPM7) S1569 Uniprot
Q96QT4 (TRPM7) T1583 Uniprot
Q96QT4 (TRPM7) S1598 Uniprot
Q96QT4 (TRPM7) S1615 Uniprot
Q96QT4 (TRPM7) S1660 Uniprot
Q96QT4 (TRPM7) T1685 Uniprot
Q96QT4 (TRPM7) S1695 Uniprot
Q96QT4 (TRPM7) S1779 Uniprot
Q96QT4 (TRPM7) T1830 Uniprot
Q96QT4 (TRPM7) S1851 Uniprot
Q96QT4 (TRPM7) S1860 Uniprot

Research Backgrounds

Function:

Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).

PTMs:

Autophosphorylated.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with PLCB1 (By similarity). Forms heterodimers with TRPM6.

Family&Domains:

In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.

In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Phosphorus magnesium fiber regulates macrophage polarization through TRPM7 to accelerate wound healing. Applied Materials Today, 2023 [IF=7.2]

Application: WB    Species: Mouse    Sample:

Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance

Application: IF/ICC    Species: Mouse    Sample:

Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance
2). Gallium ions promote osteoinduction of human and mouse osteoblasts via the TRPM7/Akt signaling pathway. Molecular Medicine Reports, 2020 (PubMed: 32945378) [IF=3.4]

Application: WB    Species: human    Sample: MC3T3 cells

Figure 3. |Effect of TRPM7 siRNAs on TRPM7 protein expression in MC3T3‑E1 and hFOB1.19 cells. (A) Total and (B) membrane TRPM7 protein expression levels in MC3T3 cells following transfection with TRPM7 siRNAs.
3). Novel benzoylurea derivative decreases TRPM7 channel function and inhibits cancer cells migration. Channels (Austin, Tex.), 2024 (PubMed: 39212541) [IF=3.3]

Application: WB    Species: Human    Sample: MCF-7 cells

Figure 4. SUD inhibits TRPM7 expression through PI3K/Akt signaling pathway. (a) Effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in MCF-7 cells examined by qPCR. (b) effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in BGC-823 cells examined by qPCR. ***, **** denote significant difference with control group, p

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