TRPM7 Antibody | Affinity Biosciences

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TRPM7 Antibody - DF7513 staining U-2 OS cells by ICC/IF.

TRPM7 Antibody - Figure 3.

TRPM7 Antibody - Fig.

TRPM7 Antibody - Figure 4.

TRPM7 Antibody - Fig.

Product:	TRPM7 Antibody
Catalog:	DF7513
Description:	Rabbit polyclonal antibody to TRPM7
Application:	WB IHC IF/ICC
Cited expt.:	WB, IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	213 KD; 213kD(Calculated).
Uniprot:	Q96QT4
RRID:	AB_2841012

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

TRPM7 Antibody detects endogenous levels of total TRPM7.

RRID:

AB_2841012
Cite Format: Affinity Biosciences Cat# DF7513, RRID:AB_2841012.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; Transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRPM7; TRPM7_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human TRPM7, corresponding to a region within C-terminal amino acids.

Uniprot:

Q96QT4(TRPM7_HUMAN) >>Visit HPA database.

Gene(ID):

TRPM7(54822)

Sequence:

Q96QT4 TRPM7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGDHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKAEMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLPITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAGKVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFHSGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDPQCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNKNQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRVSSLAGFTDCHRTSIPVHSKQAEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGLTSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

100

Horse

Bovine

Sheep

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q96QT4_TRPM7_HUMAN

Function:

Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).

PTMs:

Autophosphorylated.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Family&Domains:

In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.

In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Stem Leydig cells support macrophage immunological homeostasis through mitochondrial transfer in mice. Nature communications, 2024 (PubMed: 38459012) [IF=16.6]

Application: WB Species: Mouse Sample:

Fig. 5: TRPM7 is needed for mitochondrial transfer via TNT. A Heatmap showing the expression of pattern recognition receptors on BMSCs and SLCs. B Western blot analysis of TRPM7. C Quantitative analysis of TRPM7. Data are presented as the means ± SDs, n = 3 biological replicates for each group, one-way ANOVA. D Flow cytometry analysis of the mitochondrial transfer rate. E Quantitative analysis of the mitochondrial transfer rate. Data are presented as the means ± SDs, n = 3 biological replicates for each group, unpaired two-tailed Student’s t test. F The percentage of mitochondria transferred to macrophages treated with H2O2. G Quantitative analysis of the mitochondrial transfer rate. Data are presented as the means ± SDs. n = 3 biological replicates for each group, unpaired two-tailed Student’s t test. H The percentage of mitochondria transferred to macrophages after treatment with blebbistatin. I Quantitative analysis of the mitochondrial transfer rate. Data are presented as the means ± SDs, n = 3 biological replicates for each group, unpaired two-tailed Student’s t test. J Macrophages cocultured with SLCsMito-DsRed. Scale bar, 10 μm; large scale bar, 5 μm. K Knockdown of TRPM7 reduced mitochondrial transfer rate. L Quantitative analysis of the mitochondrial transfer rate in vivo. Data are presented as the means ± SDs. n = 3 biological replicates for each group, unpaired two-tailed t test. M Bright field diagram of testicular (scale bar, 2 mm) and H&E staining of testis samples (scale bar, 50 μm). N, O Quantification of the testis weight of the whole body and germ cell layers, n = 6 biological replicates for each group. Data are presented as the means ± SDs, one-way ANOVA. P Immunostaining of α-SMA (red) and PNA (green). Scale bar, 50 μm. Q Quantitative analysis of the PNA+ cells. n = 6 biological replicates for each group. Data are presented as the means ± SDs, one-way ANOVA. All box-and-whisker plots denote the maximum (top whisker), 75th (top edge of box), 25th (bottom edge of box), and minimum (bottom whisker) percentiles and the median (line in box). Source data are provided as a Source Data file.

2). Phosphorus magnesium fiber regulates macrophage polarization through TRPM7 to accelerate wound healing. Applied Materials Today, 2023 [IF=7.2]

Application: WB Species: Mouse Sample:

Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance

Application: IF/ICC Species: Mouse Sample:

3). Inhibition of the RIP3/MLKL/TRPM7 necroptotic pathway ameliorates diabetes mellitus-induced erectile dysfunction by reducing cell death, fibrosis, and inflammation. Frontiers in pharmacology, 2024 (PubMed: 39329120) [IF=5.6]

4). Gallium ions promote osteoinduction of human and mouse osteoblasts via the TRPM7/Akt signaling pathway. Molecular Medicine Reports, 2020 (PubMed: 32945378) [IF=3.4]

Application: WB Species: human Sample: MC3T3 cells

Figure 3. |Effect of TRPM7 siRNAs on TRPM7 protein expression in MC3T3‑E1 and hFOB1.19 cells. (A) Total and (B) membrane TRPM7 protein expression levels in MC3T3 cells following transfection with TRPM7 siRNAs.

5). Novel benzoylurea derivative decreases TRPM7 channel function and inhibits cancer cells migration. Channels (Austin, Tex.), 2024 (PubMed: 39212541) [IF=3.3]

Application: WB Species: Human Sample: MCF-7 cells

Figure 4. SUD inhibits TRPM7 expression through PI3K/Akt signaling pathway. (a) Effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in MCF-7 cells examined by qPCR. (b) effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in BGC-823 cells examined by qPCR. ***, **** denote significant difference with control group, p

6). Circ_0021155 can participate in the phenotypic transformation of human vascular smooth muscle cells via the miR-4459/TRPM7 axis. Biochemical and biophysical research communications, 2022 (PubMed: 36155059) [IF=2.5]

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TRPM7 Antibody - #DF7513