Substance P Antibody - #DF7522

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Product: Substance P Antibody
Catalog: DF7522
Description: Rabbit polyclonal antibody to Substance P
Application: WB IHC IF/ICC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 16kDa; 15kD(Calculated).
Uniprot: P20366
RRID: AB_2841021

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
Substance P Antibody detects endogenous levels of total Substance P.
RRID:
AB_2841021
Cite Format: Affinity Biosciences Cat# DF7522, RRID:AB_2841021.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C-terminal-flanking peptide; neurokinin A; neurokinin alpha; NKA; NKNA; PPT; preprotachykinin; protachykinin; protachykinin-1; SubstanceP; TAC1; TAC2; Tachykinin 1; Tachykinin precursor 1; Tachykinin1; TKN1_HUMAN; SP;

Immunogens

Immunogen:

A synthesized peptide derived from human Substance P, corresponding to a region within the internal amino acids.

Uniprot:

P20366(TKN1_HUMAN) >>Visit HPA database.

Gene(ID):
TAC1(6863)
Sequence:
MKILVALAVFFLVSTQLFAEEIGANDDLNYWSDWYDSDQIKEELPEPFEHLLQRIARRPKPQQFFGLMGKRDADSSIEKQVALLKALYGHGQISHKRHKTDSFVGLMGKRALNSVAYERSAMQNYERRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
63
Zebrafish
63
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

PTMs:

The substance P form is cleaved at Pro-59 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the tachykinin family.

References

1). Terrestrosin D ameliorates skin lesions in an imiquimod-induced psoriasis-like murine model by inhibiting the interaction between Substance P and Dendritic cells. PHYTOMEDICINE, 2022 (PubMed: 34923236) [IF=6.7]
2). Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease. Frontiers in Immunology, 2023 (PubMed: 37033943) [IF=5.7]
3). Astragalus Polysaccharides Ameliorate Diabetic Bladder Dysfunction via Normalization of Neuromuscular Conduction. Journal of agricultural and food chemistry, 2025 (PubMed: 40032633) [IF=5.7]
4). Left superior cervical ganglia lymph node mimicry and its role in rat ventricular arrhythmias following myocardial infarction. Acta physiologica (Oxford, England), 2025 (PubMed: 39803764) [IF=5.6]
5). The Effect of Shaoyao Gancao Decoction on Sphincter of Oddi Dysfunction in Hypercholesterolemic Rabbits via Protecting the Enteric Nervous System–Interstitial Cells of Cajal–Smooth Muscle Cells Network. Journal of Inflammation Research, 2021 (PubMed: 34552344) [IF=4.5]
6). Losartan attenuates acetic acid enema-induced visceral hypersensitivity by inhibiting the ACE1/Ang II/AT1 receptor axis in enteric glial cells. European journal of pharmacology, 2023 (PubMed: 36907262) [IF=4.2]
7). Zhiqiao Gancao decoction regulated JAK2/STAT3/ macrophage M1 polarization to ameliorate intervertebral disc degeneration. Heliyon, 2024 (PubMed: 39170327) [IF=4.0]

Application: WB    Species: Rat    Sample:

Fig. 2 The impact of ZQGCD on IDD rats. (A) Paw withdrawal threshold in rats. (B) Paw withdrawal latency in rats. (C) Relative expression level of substance P protein in intervertebral disc tissue of rats. The unedited images are referenced in Fig. S1 (D) Representative HE-stained intervertebral disc sections (magnification: 10 × or 40 × ). (E) Intervertebral disc histopathologic score. *: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001, ns: P>0.05.
8). Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion. Frontiers in molecular neuroscience, 2023 (PubMed: 37342099) [IF=3.5]

Application: IHC    Species: Mouse    Sample:

Figure 5. (A–D) Immunohistochemistry was used to detect the expression of CGRP, Substance P, C-Fos and KCC2 in the anterior cingulate cortex (ACC). (E1–E4) The integrated optical density (IOD) was quantitatively analyzed (IOD/area) to determine marker expression. The expression of CGRP, Substance P and C-Fos in the ACC in the SCI model increased, and the expression of KCC2 decreased (one-way ANOVA; SCI vs. sham, **P < 0.0001). The expression of CGRP, Substance P and C-Fos decreased, whereas that of KCC2 increased, after local injection of HU-MSCs (one-way ANOVA; SCI + HU-MSC vs. SCI/SCI + PBS, **P < 0.0001).
9). High-Intensity Interval Training Increases Osteoarthritis-Associated Pain-Sensitive Threshold Through Reduction of Perineuronal Nets of the Medial Prefrontal Cortex in Rats. Physiological research, 2024 (PubMed: 39903897) [IF=1.9]
10). Clinical and Animal Studies of Waist and Knee Scraping Therapy for Knee Osteoarthritis. Combinatorial chemistry & high throughput screening, 2024 (PubMed: 38284729) [IF=1.6]
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