Substance P Antibody | Affinity Biosciences

WB
IHC
Cites

1/5

Substance P Antibody - Western blot analysis of extracts from mouse spleen tissue, using Substance P Antibody.

Substance P Antibody - DF7522 at 1/100 staining Mouse testis tissue by IHC-P.

Substance P Antibody - Figure 5: | Acupuncture needling and electroacupuncture reduced the expression levels of Neurokinin A (NKA) in IMQ-induced mouse skin lesions.

Substance P Antibody - Fig.

Substance P Antibody - Figure 4 Effects of electroacupuncture on the expression of SP and CGRP neurotransmitters in the colon tissue of Thy1-αSyn transgenic mice.

Product:	Substance P Antibody
Catalog:	DF7522
Description:	Rabbit polyclonal antibody to Substance P
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	16kDa; 15kD(Calculated).
Uniprot:	P20366
RRID:	AB_2841021

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

Substance P Antibody detects endogenous levels of total Substance P.

RRID:

AB_2841021
Cite Format: Affinity Biosciences Cat# DF7522, RRID:AB_2841021.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C-terminal-flanking peptide; neurokinin A; neurokinin alpha; NKA; NKNA; PPT; preprotachykinin; protachykinin; protachykinin-1; SubstanceP; TAC1; TAC2; Tachykinin 1; Tachykinin precursor 1; Tachykinin1; TKN1_HUMAN; SP;

Immunogens

Immunogen:

Uniprot:

P20366(TKN1_HUMAN) >>Visit HPA database.

Gene(ID):

TAC1(6863)

Sequence:

P20366 TKN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKILVALAVFFLVSTQLFAEEIGANDDLNYWSDWYDSDQIKEELPEPFEHLLQRIARRPKPQQFFGLMGKRDADSSIEKQVALLKALYGHGQISHKRHKTDSFVGLMGKRALNSVAYERSAMQNYERRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P20366_TKN1_HUMAN

Function:

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

PTMs:

The substance P form is cleaved at Pro-59 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

Subcellular Location:

Secreted.

Family&Domains:

Belongs to the tachykinin family.

References

1). Terrestrosin D ameliorates skin lesions in an imiquimod-induced psoriasis-like murine model by inhibiting the interaction between Substance P and Dendritic cells. PHYTOMEDICINE, 2022 (PubMed: 34923236) [IF=6.7]

2). Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease. Frontiers in Immunology, 2023 (PubMed: 37033943) [IF=5.7]

3). The Effect of Shaoyao Gancao Decoction on Sphincter of Oddi Dysfunction in Hypercholesterolemic Rabbits via Protecting the Enteric Nervous System–Interstitial Cells of Cajal–Smooth Muscle Cells Network. Journal of Inflammation Research, 2021 (PubMed: 34552344) [IF=4.5]

4). Zhiqiao Gancao decoction regulated JAK2/STAT3/ macrophage M1 polarization to ameliorate intervertebral disc degeneration. Heliyon, 2024 (PubMed: 39170327) [IF=4.0]

Application: WB Species: Rat Sample:

Fig. 2 The impact of ZQGCD on IDD rats. (A) Paw withdrawal threshold in rats. (B) Paw withdrawal latency in rats. (C) Relative expression level of substance P protein in intervertebral disc tissue of rats. The unedited images are referenced in Fig. S1 (D) Representative HE-stained intervertebral disc sections (magnification: 10 × or 40 × ). (E) Intervertebral disc histopathologic score. *: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001, ns: P>0.05.

5). Clinical and Animal Studies of Waist and Knee Scraping Therapy for Knee Osteoarthritis. Combinatorial chemistry & high throughput screening, 2024 (PubMed: 38284729) [IF=1.6]

6). Acupuncture Needling, Electroacupuncture, and Fire Needling Improve Imiquimod-Induced Psoriasis-Like Skin Lesions through Reducing Local Inflammatory Responses. Evidence-based Complementary and Alternative Medicine, 2019 (PubMed: 31467575)

Application: WB Species: mouse Sample: skin

Figure 5: | Acupuncture needling and electroacupuncture reduced the expression levels of Neurokinin A (NKA) in IMQ-induced mouse skin lesions. Te expression levels of (a) NKA, and (b) Substance P (SP) were detected by Western immunoblotting. Data are presented as the mean ± SD (n = 7/group). ##p<0.01, ###p<0.001 vs Ctrl; ∗p <0.05, ∗∗p <0.01 vs model.

7). Electroacupuncture Modulates 5-HT4R-Mediated cAMP/PKA Signaling to Improve Intestinal Motility Disorders in a Thy1- α Syn Parkinson's Mouse Model. Evidence-based complementary and alternative medicine : eCAM, 2022 (PubMed: 36337584)

Application: WB Species: Mouse Sample: colon tissue

Figure 4 Effects of electroacupuncture on the expression of SP and CGRP neurotransmitters in the colon tissue of Thy1-αSyn transgenic mice. (a) mRNA expression of SP. (b) mRNA expression of CGRP. (c) Representative western blot images showing the protein expression levels of SP and CGRP. (d) Protein expression in the mouse colon tissue of the normal control (N), PD model (M), electroacupuncture (EA), and western medicine (WM) groups. ∗P < 0.05 and ∗∗P < 0.01 relative to the normal control group; #P < 0.05 and ##P < 0.01 relative to the PD model group.

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Substance P Antibody - #DF7522