RNF14 Antibody | Affinity Biosciences

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Product:	RNF14 Antibody
Catalog:	AF0819
Description:	Rabbit polyclonal antibody to RNF14
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	50kDa; 54kD(Calculated).
Uniprot:	Q9UBS8
RRID:	AB_2834384

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%)

Clonality:

Polyclonal

Specificity:

RNF14 Antibody detects endogenous levels of total RNF14.

RRID:

AB_2834384
Cite Format: Affinity Biosciences Cat# AF0819, RRID:AB_2834384.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Androgen receptor associated protein 54; Androgen receptor-associated protein 54; ARA 54; ARA54; E3 ubiquitin protein ligase RNF14; E3 ubiquitin-protein ligase RNF14; FLJ26004; HFB 30; HFB30; HRIHFB2038; RING finger protein 14; RNF 14; Rnf14; RNF14_HUMAN; TRIAD 2; TRIAD2; Triad2 protein;

Immunogens

Immunogen:

Uniprot:

Q9UBS8(RNF14_HUMAN) >>Visit HPA database.

Gene(ID):

RNF14(9604)

Expression:

Q9UBS8 RNF14_HUMAN:

Widely expressed.

Description:

RNF14 Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. Belongs to the RBR family. RNF14 subfamily. Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2 and in the presence of testosterone, with the androgen receptor (AR).

Sequence:

Q9UBS8 RNF14_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSEDREAQEDELLALASIYDGDEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNECLQNSGFEYTICFLPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLWEEHRGSVVLFAWMQFLKEETLAYLNIVSPFELKIGSQKKVQRRTAQASPNTELDFGGAAGSDVDQEEIVDERAVQDVESLSNLIQEILDFDQAQQIKCFNSKLFLCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATPGQVKELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNFAFCTLCRLTYHGVSPCKVTAEKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKLDGCNKMTCTGCMQYFCWICMGSLSRANPYKHFNDPGSPCFNRLFYAVDVDDDIWEDEVED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Zebrafish

Xenopus

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBS8 As Substrate

Q9UBS8

Site	PTM Type	Source
K27	Ubiquitination	Uniprot
S162	Phosphorylation	Uniprot
T165	Phosphorylation	Uniprot
S175	Phosphorylation	Uniprot
S230	Phosphorylation	Uniprot
Y242	Phosphorylation	Uniprot
K269	Ubiquitination	Uniprot
S348	Phosphorylation	Uniprot
K351	Ubiquitination	Uniprot
K356	Ubiquitination	Uniprot
K372	Ubiquitination	Uniprot
Y379	Phosphorylation	Uniprot
K386	Ubiquitination	Uniprot
S393	Phosphorylation	Uniprot
K394	Ubiquitination	Uniprot
K399	Ubiquitination	Uniprot
K402	Ubiquitination	Uniprot
T409	Phosphorylation	Uniprot
K444	Ubiquitination	Uniprot
S451	Phosphorylation	Uniprot

Research Backgrounds

Q9UBS8_RNF14_HUMAN

Function:

Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.

PTMs:

RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor; testosterone- and RNF6-regulated it promotes AR transcriptional activity.

Family&Domains:

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.

The RING-type zinc finger is essential for the interaction with UBE2E2.

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.

Belongs to the RBR family. RNF14 subfamily.

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RNF14 Antibody - #AF0819