GM130 Antibody - #DF7556

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Product: GM130 Antibody
Catalog: DF7556
Description: Rabbit polyclonal antibody to GM130
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Chicken
Mol.Wt.: 130 kDa; 113kD(Calculated).
Uniprot: Q08379
RRID: AB_2841050

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:50-200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Chicken(80%)
Clonality:
Polyclonal
Specificity:
GM130 Antibody detects endogenous levels of total GM130.
RRID:
AB_2841050
Cite Format: Affinity Biosciences Cat# DF7556, RRID:AB_2841050.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

130 kDa cis Golgi matrix protein; 130 kDa cis-Golgi matrix protein; Cis golgi matrix protein GM130; GM130; Gm130 autoantigen; GOGA2_HUMAN; GOLGA 2; Golga2; Golgi autoantigen; Golgi autoantigen golgin subfamily a 2; Golgi matrix protein GM130; Golgin 95; golgin A2; Golgin subfamily a 2; Golgin subfamily A member 2; Golgin-95; MGC20672; SY11 protein;

Immunogens

Immunogen:
Uniprot:

Q08379(GOGA2_HUMAN) >>Visit HPA database.

Gene(ID):
GOLGA2(2801)
Sequence:
MWPQPRLPPRPAMSEETRQSKLAAAKKKLREYQQRNSPGVPTGAKKKKKIKNGSNPETTTSGGCHSPEDTPKDNAATLQPSDDTVLPGGVPSPGASLTSMAASQNHDADNVPNLMDETKTFSSTESLRQLSQQLNGLVCESATCVNGEGPASSANLKDLESRYQQLAVALDSSYVTNKQLNITIEKLKQQNQEITDQLEEEKKECHQKQGALREQLQVHIQTIGILVSEKAELQTALAHTQHAARQKEGESEDLASRLQYSRRRVGELERALSAVSTQQKKADRYNKELTKERDALRLELYKNTQSNEDLKQEKSELEEKLRVLVTEKAGMQLNLEELQKKLEMTELLLQQFSSRCEAPDANQQLQQAMEERAQLEAHLGQVMESVRQLQMERDKYAENLKGESAMWRQRMQQMSEQVHTLREEKECSMSRVQELETSLAELRNQMAEPPPPEPPAGPSEVEQQLQAEAEHLRKELEGLAGQLQAQVQDNEGLSRLNREQEERLLELERAAELWGEQAEARRQILETMQNDRTTISRALSQNRELKEQLAELQSGFVKLTNENMEITSALQSEQHVKRELGKKLGELQEKLSELKETVELKSQEAQSLQQQRDQYLGHLQQYVAAYQQLTSEKEVLHNQLLLQTQLVDQLQQQEAQGKAVAEMARQELQETQERLEAATQQNQQLRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
80
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08379 As Substrate

Site PTM Type Enzyme
R18 Methylation
S20 Phosphorylation
R30 Methylation
R35 Methylation
S37 Phosphorylation P06493 (CDK1)
T42 Phosphorylation
K47 Acetylation
K51 Ubiquitination
S54 Phosphorylation
T58 Phosphorylation
S66 Phosphorylation
S92 Phosphorylation
S122 Phosphorylation
S131 Phosphorylation
S152 Phosphorylation
Y174 Phosphorylation
T195 Phosphorylation
K202 Ubiquitination
S251 Phosphorylation
S273 Phosphorylation
K280 Ubiquitination
K302 Ubiquitination
K320 Ubiquitination
S438 Phosphorylation
K474 Ubiquitination
R532 Methylation
S540 Phosphorylation
K546 Ubiquitination
K590 Ubiquitination
K595 Ubiquitination
S690 Phosphorylation
S774 Phosphorylation
K777 Ubiquitination
S792 Phosphorylation
K807 Ubiquitination
K819 Ubiquitination
Y850 Phosphorylation
K881 Ubiquitination
S937 Phosphorylation
S941 Phosphorylation
S953 Phosphorylation
T959 Phosphorylation
S981 Phosphorylation

Research Backgrounds

Function:

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization. Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle. Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization. Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins.

PTMs:

Cleaved by caspases at the onset of apoptosis.

Methylation by PRMT5 is required for Golgi ribbon formation. While dimethylation at Arg-30 and Arg-35 are confirmed in vivo, it is unclear whether Arg-18 is methylated in vivo.

Phosphorylated at Ser-37 by CDK1 at the onset of mitosis, inhibiting the interaction with p115/USO1 and triggering Golgi disassembly. Phosphorylated at Ser-37 in prophase as the Golgi complex starts to break down, and remains phosphorylated during further breakdown and partitioning of the Golgi fragments in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble (By similarity).

Subcellular Location:

Golgi apparatus>cis-Golgi network membrane>Peripheral membrane protein>Cytoplasmic side. Endoplasmic reticulum-Golgi intermediate compartment membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Associates with the mitotic spindle during mitosis (PubMed:26165940).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer, may assemble into homohexamers. Homotetramer; forms a parallel homotetramer with a flexible rod-like structure that can give rise to I- and Y-shaped conformations (By similarity). Interacts with GORASP1/GRASP65. The homooligomer forms a complex with GORASP1 with a 1:1 stoichiometry (By similarity). Interacts with RAB1B that has been activated by GTP-binding. Interacts with p115/USO1; interaction with p115/USO1 inhibits interaction with STX5 and/or RAB1B. Interacts with STX5 (By similarity). Interacts with ZFPL1. Interacts with AKAP450/AKAP9; leading to recruit AKAP450/AKAP9 to the cis-Golgi.

Family&Domains:

Extended rod-like protein with long coiled-coil domains.

The nuclear localization signal (cNLS) mediates interaction with importin-alpha, recruiting importin-alpha to the Golgi membrane and liberating TPX2.

Belongs to the GOLGA2 family.

References

1). Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38014604) [IF=15.1]

Application: WB    Species: Mouse    Sample:

Figure 3 Arf1 participated in Golgi apparatus distribution during mouse oocyte meiosis. A) Representative images of Golgi distribution at the GV stage in the control and Arf1‐KD oocytes. Red, Golgi; blue, DNA. Scale bar = 5 µm. B) The rate of abnormal Golgi distribution in GV oocytes significantly increased after Arf1 depletion. C) Representative images of Golgi distribution in the GV stage in the control and Myt1‐KD oocytes. Red, Golgi; blue, DNA. Scale bar = 5 µm. D) Screening vesicle transport–related proteins connected with Arf1 by mass spectrometry analysis. E) Co‐IP results indicated that Arf1 was associated with cis‐Golgi marker GM130, GTPase Rab35, and Miro2, but not Rab1A. F) Western blot analysis showed a significant decrease in the GM130 and Rab35 protein levels in the Arf1‐KD oocytes compared with the control oocytes. Western blot analysis revealed no difference in Miro2 expression between the control and Arf1‐KD groups. Overall, 180 oocytes were used in each group. G) Representative images of GM130 distribution in the GV stage in the control and Arf1‐KD oocytes. The fluorescence intensity of the GM130 signal significantly decreased after Arf1 depletion. Green, GM130; blue, DNA. Scale bar = 20 µm. H) Representative images of Rab35 distribution in the GV stage in the control and Arf1‐KD oocytes. The fluorescence intensity of the Rab35 signal significantly decreased after Arf1 depletion. Red, Rab35; blue, DNA. Scale bar = 20 µm. I) Representative images of Golgi distribution in the MI stage in the control and Arf1‐KD oocytes. Red, Golgi; blue, DNA. Scale bar = 5 µm. J) The rate of abnormal Golgi distribution in MI oocytes significantly increased in the Arf1‐KD group compared with the control group. The data are presented as mean ± SEM from at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.
2). The role of S100B/RAGE-enhanced ADAM17 activation in endothelial glycocalyx shedding after traumatic brain injury. Journal of Neuroinflammation, 2022 (PubMed: 35148784) [IF=9.3]

Application: IF/ICC    Species: Rat    Sample: RAECs

Fig. 8 Activating S100B/RAGE signal promotes ADAM17 translocation in RAECs. A Representative confocal images showing the subcellular localization of ADAM17 and GM130 (the Golgi complex-associated protein) in RAECs treated with stretch injured-conditioned medium, exogenous S100B, and injured medium plus S100B inhibitor ONO-2506 or RAGE inhibitor FPS-ZM-1. Scale bar = 20 μm. The immunofluorescence intensity of GM130+/ADAM17+ in cell from five fields per sample in each group was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with control group, #p < 0.05 compared with conditioned medium-treated group, n = 3. B Representative confocal images showing the subcellular localization of ADAM17 and vWF in RAECs treated with stretch injured-conditioned medium, exogenous S100B, and injured medium plus ONO-2506 or FPS-ZM-1. Scale bar = 20 μm. The immunofluorescence intensity of vWF+/ADAM17+ in cells from five fields per sample in each group was quantified and reported as relative fluorescence units (RFUs). *p < 0.05 compared with control group, #p < 0.05 compared with conditioned medium-treated group, n = 3
3). Arf6 GTPase deficiency leads to porcine oocyte quality decline during aging. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2024 (PubMed: 38884157) [IF=4.4]

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