PDI Antibody - #DF7593

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Product: PDI Antibody
Catalog: DF7593
Description: Rabbit polyclonal antibody to PDI
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 61 kDa; 58kD(Calculated).
Uniprot: Q13087
RRID: AB_2841084

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
PDI Antibody detects endogenous levels of total PDI.
RRID:
AB_2841084
Cite Format: Affinity Biosciences Cat# DF7593, RRID:AB_2841084.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Pancreas-specific protein disulfide isomerase; pancreatic protein disulfide isomerase; PDIA2; PDIA2_HUMAN; PDIp; PDIR; protein disulfide isomerase family A member 2; protein disulfide isomerase, pancreatic; protein disulfide isomerase-associated 2; Protein disulfide-isomerase A2; Rho GDP dissociation inhibitor gamma;

Immunogens

Immunogen:
Uniprot:

Q13087(PDIA2_HUMAN) >>Visit HPA database.

Gene(ID):
PDIA2(64714)
Expression:
Q13087 PDIA2_HUMAN:

Highly expressed in pancreas (at protein level).

Sequence:
MSRQLLPVLLLLLLRASCPWGQEQGARSPSEEPPEEEIPKEDGILVLSRHTLGLALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDGPAQRELAEEFGVTEYPTLKFFRNGNRTHPEEYTGPRDAEGIAEWLRRRVGPSAMRLEDEAAAQALIGGRDLVVIGFFQDLQDEDVATFLALAQDALDMTFGLTDRPRLFQQFGLTKDTVVLFKKFDEGRADFPVDEELGLDLGDLSRFLVTHSMRLVTEFNSQTSAKIFAARILNHLLLFVNQTLAAHRELLAGFGEAAPRFRGQVLFVVVDVAADNEHVLQYFGLKAEAAPTLRLVNLETTKKYAPVDGGPVTAASITAFCHAVLNGQVKPYLLSQEIPPDWDQRPVKTLVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDIIIAELDATANELDAFAVHGFPTLKYFPAGPGRKVIEYKSTRDLETFSKFLDNGGVLPTEEPPEEPAAPFPEPPANSTMGSKEEL

PTMs - Q13087 As Substrate

Site PTM Type Enzyme
N127 N-Glycosylation
N284 N-Glycosylation
T286 Phosphorylation
K479 Acetylation
N516 N-Glycosylation

Research Backgrounds

Function:

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.

PTMs:

The disulfide-linked homodimer exhibits an enhanced chaperone activity.

Glycosylated.

Subcellular Location:

Endoplasmic reticulum lumen.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in pancreas (at protein level).

Subunit Structure:

Monomer; predominantly as monomer under reducing conditions. Homodimer; disulfide-linked. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

Family&Domains:

Belongs to the protein disulfide isomerase family.

References

1). Modulating endoplasmic reticulum stress in APP/PS1 mice by Gomisin B and Osthole in Bushen-Yizhi formula: Synergistic effects and therapeutic implications for Alzheimer's disease. Phytomedicine, 2023 (PubMed: 37586159) [IF=6.7]

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