SREBP2 Antibody - #DF7601

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Product: SREBP2 Antibody
Catalog: DF7601
Description: Rabbit polyclonal antibody to SREBP2
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 124kDa; 124kD(Calculated).
Uniprot: Q12772
RRID: AB_2841092

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SREBP2 Antibody detects endogenous levels of total SREBP2.
RRID:
AB_2841092
Cite Format: Affinity Biosciences Cat# DF7601, RRID:AB_2841092.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI608257; bHLHd2; Class D basic helix-loop-helix protein 2; OTTHUMP00000028740; Processed sterol regulatory element-binding protein 2; SRBP2_HUMAN; SREBF 2; Srebf2; SREBF2 protein; SREBP 2; SREBP-2; SREBP2; SREBP2gc; sterol regulatory element binding factor 2; Sterol regulatory element binding protein 2; Sterol regulatory element binding transcription factor 2; Sterol regulatory element-binding transcription factor 2;

Immunogens

Immunogen:

A synthesized peptide derived from human SREBP2, corresponding to a region within the internal amino acids.

Uniprot:

Q12772(SRBP2_HUMAN) >>Visit HPA database.

Gene(ID):
SREBF2(6721)
Expression:
Q12772 SRBP2_HUMAN:

Ubiquitously expressed in adult and fetal tissues.

Sequence:
MDDSGELGGLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGSGGSGSSSGSSGSSSSSSNGRGSSSGAVDPSVQRSFTQVTLPSFSPSAASPQAPTLQVKVSPTSVPTTPRATPILQPRPQPQPQPQTQLQQQTVMITPTFSTTPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSSGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLKIEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTSLLQWGGAHDSDQHPHSGSGRSVLSFESGSGGWFDWMMPTLLLWLVNGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAGNLQTCLAVLGRALPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQCRRATPATEAGFEDEAKTSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTRCGGKLGFLASYFLSRAQSLCGPEHSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFTKVERIPKALEVTESPLVKAIFHACRAMHASLPGKADGQQSSFCHCERASGHLWSSLNVSGATSDPALNHVVQLLTCDLLLSLRTALWQKQASASQAVGETYHASGAELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTTQSTKHGEVDAWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCNDCQQMIVKLGGGTAIAAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

PTMs:

At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.

Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in adult and fetal tissues.

Family&Domains:

Belongs to the SREBP family.

References

1). CCDC92 deficiency ameliorates podocyte lipotoxicity in diabetic kidney disease. Metabolism: clinical and experimental, 2024 (PubMed: 37952690) [IF=10.8]
2). NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes. LIFE SCIENCES, 2020 (PubMed: 32976883) [IF=5.2]

Application: WB    Species: rat    Sample: BRL-3A hepatocytes

Fig. 3. |NPY promotes pSREBP2 expression and accelerates the processing of pSREBP2 to mSREBP2 both in vivo and in vitro.(A) Hepatic portal vein injection of NPY (100 μg/kg) significantly increased both pSREBP2 expression and mSREBP2 maturation after 1 h in rats.
3). Digeda-4 decoction and its disassembled prescriptions improve dyslipidemia and apoptosis by regulating AMPK/SIRT1 pathway on tyloxapol-induced nonalcoholic fatty liver disease in mice. Journal of Ethnopharmacology, 2023 (PubMed: 37348794) [IF=4.8]
4). Alcohol intake exacerbates experimental autoimmune prostatitis through gut microbiota driving cholesterol biosynthesis-mediated Th17 differentiation. International immunopharmacology, 2024 (PubMed: 39029231) [IF=4.8]
5). Resveratrol Improves the Progression of Osteoarthritis by Regulating the SIRT1-FoxO1 Pathway-Mediated Cholesterol Metabolism. MEDIATORS OF INFLAMMATION, 2023 (PubMed: 36643587) [IF=4.4]
6). Hypermethylation of the CTRP9 promoter region promotes Hcy induced VSMC lipid deposition and foam cell formation via negatively regulating ER stress. Scientific reports, 2023 (PubMed: 37945738) [IF=3.8]

Application: WB    Species: Human    Sample: VSMC

Figure 4. Effects of CTRP9 on the expression of ERs marker proteins and lipid homeostasis regulatory factors in VSMC. (A) CTRP9 mRNA and protein expression levels. (B) ATF6a mRNA and protein expression levels. (C) mRNA and protein expression levels of CRP78. (D) Expression levels of IRE1a mRNA and p-IRE1a protein. (E) Expression levels of PERK mRNA and p-PERK protein. (F) SREBP1c mRNA and protein expression levels. (G) SREBP2 mRNA and protein expression levels. The experiment was performed in triplicate, and the representative images are shown. *P 
7). MiR-204-5p promotes apoptosis and inhibits migration of osteosarcoma via targeting EBF2. BIOCHIMIE, 2019 (PubMed: 30529043) [IF=3.3]

Application: WB    Species: human    Sample: MG63 cells

Figure 4.| MiR-204-5p targets EBF2 .D. WB analysis of EBF2 protein levels in MG63 cells transfected with control miRNA (miR-NC), miR-204-5p, control antagomiR (anti-miR-NC) and antagomiR-204-5p (anti-miR-204-5p).

Application: IHC    Species: human    Sample: Migrated Saos-2 and MG63 cells

Figure 5. |EBF2 inhibits apoptosis and promotes migration and invasion of osteosarcoma.E. Migrated Saos-2 and MG63 cells transfected with control siRNA (si-NC) and EBF2 siRNA (si-EBF2). Lower panels are statistics of transwell results. Data were presented as means ± SD. *=P<0.05, **=P<0.01 and ***=P<0.001
8). Sleeve Gastrectomy Suppresses Hepatic De Novo Cholesterogenesis and Improves Hepatic Cholesterol Accumulation in Obese Rats with Type 2 Diabetes Mellitus. NUTRITION, 2022 (PubMed: 34952362) [IF=3.2]
9). Metformin mitigates cholesterol accumulation via the AMPK/SIRT1 pathway to protect osteoarthritis chondrocytes. Biochemical and biophysical research communications, 2022 (PubMed: 36206595) [IF=2.5]
10). The Hypolipidemic Effect of Dalbergia odorifera T. C. Chen Leaf Extract on Hyperlipidemic Rats and Its Mechanism Investigation Based on Network Pharmacology. Evidence-Based Complementary and Alternative Medicine, 2021 (PubMed: 34987591)

Application: WB    Species: Rat    Sample: liver

Figure 9 Western blotting. Effect of D. odorifera leaves on the protein levels of SCAP, SREBP-2, HMGCR, and LDLR in the liver.
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