DAP Antibody - #DF7628

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Product: DAP Antibody
Catalog: DF7628
Description: Rabbit polyclonal antibody to DAP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Chicken
Mol.Wt.: 11KD; 11kD(Calculated).
Uniprot: P51397
RRID: AB_2841113

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
DAP Antibody detects endogenous levels of total DAP.
RRID:
AB_2841113
Cite Format: Affinity Biosciences Cat# DF7628, RRID:AB_2841113.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DAP 1; Dap; DAP-1; DAP1_HUMAN; Death associated protein 1; Death associated protein; Death-associated protein 1; MGC99796; OTTHUMP00000161670; OTTHUMP00000221331;

Immunogens

Immunogen:
Uniprot:

P51397(DAP1_HUMAN) >>Visit HPA database.

Gene(ID):
DAP(1611)
Sequence:
MSSPPEGKLETKAGHPPAVKAGGMRIVQKHPHTGDTKEEKDKDDQEWESPSPPKPTVFISGVIARGDKDFPPAAAQVAHQKPHASMDKHPSPRTQHIQQPRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Chicken
83
Zebrafish
75
Xenopus
67
Horse
0
Sheep
0
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51397 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S3 Phosphorylation
K8 Ubiquitination
K12 Ubiquitination
K20 Acetylation
K29 Ubiquitination
S49 Phosphorylation
S51 Phosphorylation P42345 (MTOR)
K54 Ubiquitination
T56 Phosphorylation
S60 Phosphorylation
K68 Acetylation
K81 Acetylation
S85 Phosphorylation
S91 Phosphorylation
R93 Methylation

Research Backgrounds

Function:

Negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death.

PTMs:

Phosphorylated. Phosphorylation by MTOR inhibits the suppressive activity of DAP toward autophagy.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.