PLEC Antibody - #DF7631
(1)
| Product: | PLEC Antibody |
| Catalog: | DF7631 |
| Description: | Rabbit polyclonal antibody to PLEC |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Bovine, Horse |
| Mol.Wt.: | 531 KD; 532kD(Calculated). |
| Uniprot: | Q15149 |
| RRID: | AB_2841116 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%)
Clonality:
Polyclonal
Specificity:
PLEC Antibody detects endogenous levels of total PLEC.
RRID:
AB_2841116
Cite Format: Affinity Biosciences Cat# DF7631, RRID:AB_2841116.
Cite Format: Affinity Biosciences Cat# DF7631, RRID:AB_2841116.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
EBS1; EBSO; HD1; Hemidesmosomal protein 1; PCN; pleC; PLEC_HUMAN; PLEC1; PLEC1b; Plectin 1; Plectin 1 intermediate filament binding protein 500kDa; Plectin 6; Plectin; Plectin-1; PLTN;
Immunogens
Immunogen:
A synthesized peptide derived from human PLEC, corresponding to a region within N-terminal amino acids.
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q15149 PLEC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
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Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Bovine
100
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Function:
Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.
PTMs:
Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
Subcellular Location:
Cytoplasm>Cytoskeleton. Cell junction>Hemidesmosome.
Tissue Specificity:
Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
Family&Domains:
The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N- and the C-terminus can bind integrin beta-4.
Belongs to the plakin or cytolinker family.
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