TUBGCP5 Antibody - #AF0621

(3)  
Product: TUBGCP5 Antibody
Catalog: AF0621
Description: Rabbit polyclonal antibody to TUBGCP5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 118kDa; 118kD(Calculated).
Uniprot: Q96RT8
RRID: AB_2834398

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
TUBGCP5 Antibody detects endogenous levels of total TUBGCP5.
RRID:
AB_2834398
Cite Format: Affinity Biosciences Cat# AF0621, RRID:AB_2834398.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gamma tubulin complex component GCP5; Gamma-tubulin complex component 5; GCP 5; GCP-5; GCP5; GCP5_HUMAN; KIAA1899; OTTHUMP00000159254; Tubgcp5; Tubulin gamma complex associated protein 5;

Immunogens

Immunogen:
Uniprot:

Q96RT8(GCP5_HUMAN) >>Visit HPA database.

Gene(ID):
TUBGCP5(114791)
Expression:
Q96RT8 GCP5_HUMAN:

Widely expressed, with highest levels in heart and skeletal muscle and moderate levels in brain.

Description:
GCP5 Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. Belongs to the GCP family. Gamma-tubulin complex is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6.
Sequence:
MARHGPPWSRLDAQQERDVRELVRGVAGLQDEADPNFQLALNFAWSNFRFHRFLDVNSHKIEKTIEGIYEKFVIHSDLSKAASWKRLTEEFLNAPLPSIKEIKTDAHYSILSLLLCLSDSPSNSSYVETPRNKEVEKKDDFDWGKYLMEDEEMDIGPYMDTPNWSEESEEENDQQPLSREDSGIQVDRTPLEEQDQNRKLDPCISWKDEPDDRSWLEHHVVHQYWTARPSQFPHSLHLHSNLAAVWDQHLYSSDPLYVPDDRVLVTETQVIRETLWLLSGVKKLFIFQLIDGKVTVRNNIIVTHLTHSCLRSVLEQIAAYGQVVFRLQEFIDEVMGHSSESMLPGSGSVPKKSTEAPFRTYQAFMWALYKYFISFKEELAEIEKCIINNDTTITLAIVVDKLAPRLSQLKVLHKVFSTGVAEVPPDTRNVVRASHLLNTLYKAILEYDNVGEASEQTVSLLFSLWVETVRPYLQTVDEWIVHGHLWDGAREFIIQRNKNVPVNHRDFWYATYTLYSVSEKTENEEKMSDNASASSGSDQGPSSRQHTMVSFLKPVLKQIIMAGKSMQLLKNLQCAESTTCQAGARDAERKSLYTLFLESVQSRLRHGEDSTPQVLTEQQATKENLMKMQSIAESHLELDDVHDPLLAINFARMYLEQSDFHEKFAGGDVCVDRSSESVTCQTFELTLRSCLYPHIDKQYLDCCGNLMQTLKKDYRLVEYLQAMRNFFLMEGGDTMYDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSRLSISFENVDTAKKKLPVHILDGLTLSYKVPWPVDIVISLECQKIYNQVFLLLLQIKWAKYSLDVLLFGELVSTAEKPRLKEGLIHEQDTVAQFGPQKEPVRQQIHRMFLLRVKLMHFVNSLHNYIMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSFVKEAIMKVLNLALMFADGWQAGLGTWRMESIEKMESDFKNCHMFLVTILNKAVCRGSFPHLESLALSLMAGMEQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
91
Sheep
91
Chicken
91
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RT8 As Substrate

Site PTM Type Enzyme
K63 Ubiquitination
Y69 Phosphorylation
S83 Phosphorylation
K100 Ubiquitination
K138 Ubiquitination
S182 Phosphorylation
R188 Methylation
S205 Phosphorylation
S346 Phosphorylation
S348 Phosphorylation
S407 Phosphorylation
K410 Ubiquitination
K526 Ubiquitination
S528 Phosphorylation
S532 Phosphorylation
S535 Phosphorylation
K553 Ubiquitination
K564 Ubiquitination
K570 Ubiquitination
K590 Ubiquitination
T611 Phosphorylation
K622 Ubiquitination
S630 Phosphorylation
S658 Phosphorylation
K663 Ubiquitination
K697 Ubiquitination
K711 Ubiquitination
K788 Ubiquitination
K789 Ubiquitination
K856 Ubiquitination
K873 Ubiquitination

Research Backgrounds

Function:

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest levels in heart and skeletal muscle and moderate levels in brain.

Subunit Structure:

Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6.

Family&Domains:

Belongs to the TUBGCP family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.