CENPE Antibody - #DF7745

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Product: CENPE Antibody
Catalog: DF7745
Description: Rabbit polyclonal antibody to CENPE
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Dog
Mol.Wt.: 312 kDa; 316kD(Calculated).
Uniprot: Q02224
RRID: AB_2841212

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(89%), Dog(91%)
Clonality:
Polyclonal
Specificity:
CENPE Antibody detects endogenous levels of total CENPE.
RRID:
AB_2841212
Cite Format: Affinity Biosciences Cat# DF7745, RRID:AB_2841212.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CENP E; Centromere associated protein E; Centromere autoantigen E (312kD); Centromere autoantigen E; Centromere protein E 312kDa; Centromere protein E; KIF10; Kinesin family member 10; Kinesin related protein; Kinesin related protein CENPE; PPP1R61; Protein phosphatase 1, regulatory subunit 61;

Immunogens

Immunogen:
Uniprot:

Q02224(CENPE_HUMAN) >>Visit HPA database.

Gene(ID):
CENPE(1062)
Sequence:
MAEEGAVAVCVRVRPLNSREESLGETAQVYWKTDNNVIYQVDGSKSFNFDRVFHGNETTKNVYEEIAAPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSEDHLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCGTQKMKPLIIREDVNRNVYVADLTEEVVYTSEMALKWITKGEKSRHYGETKMNQRSSRSHTIFRMILESREKGEPSNCEGSVKVSHLNLVDLAGSERAAQTGAAGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTITPVSFDETLTALQFASTAKYMKNTPYVNEVSTDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQNEKIENLTRMLVTSSSLTLQQELKAKRKRRVTWCLGKINKMKNSNYADQFNIPTNITTKTHKLSINLLREIDESVCSESDVFSNTLDTLSEIEWNPATKLLNQENIESELNSLRADYDNLVLDYEQLRTEKEEMELKLKEKNDLDEFEALERKTKKDQEMQLIHEISNLKNLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQEYIDSQKLENIKMDLSYSLESIEDPKQMKQTLFDAETVALDAKRESAFLRSENLELKEKMKELATTYKQMENDIQLYQSQLEAKKKMQVDLEKELQSAFNEITKLTSLIDGKVPKDLLCNLELEGKITDLQKELNKEVEENEALREEVILLSELKSLPSEVERLRKEIQDKSEELHIITSEKDKLFSEVVHKESRVQGLLEEIGKTKDDLATTQSNYKSTDQEFQNFKTLHMDFEQKYKMVLEENERMNQEIVNLSKEAQKFDSSLGALKTELSYKTQELQEKTREVQERLNEMEQLKEQLENRDSTLQTVEREKTLITEKLQQTLEEVKTLTQEKDDLKQLQESLQIERDQLKSDIHDTVNMNIDTQEQLRNALESLKQHQETINTLKSKISEEVSRNLHMEENTGETKDEFQQKMVGIDKKQDLEAKNTQTLTADVKDNEIIEQQRKIFSLIQEKNELQQMLESVIAEKEQLKTDLKENIEMTIENQEELRLLGDELKKQQEIVAQEKNHAIKKEGELSRTCDRLAEVEEKLKEKSQQLQEKQQQLLNVQEEMSEMQKKINEIENLKNELKNKELTLEHMETERLELAQKLNENYEEVKSITKERKVLKELQKSFETERDHLRGYIREIEATGLQTKEELKIAHIHLKEHQETIDELRRSVSEKTAQIINTQDLEKSHTKLQEEIPVLHEEQELLPNVKEVSETQETMNELELLTEQSTTKDSTTLARIEMERLRLNEKFQESQEEIKSLTKERDNLKTIKEALEVKHDQLKEHIRETLAKIQESQSKQEQSLNMKEKDNETTKIVSEMEQFKPKDSALLRIEIEMLGLSKRLQESHDEMKSVAKEKDDLQRLQEVLQSESDQLKENIKEIVAKHLETEEELKVAHCCLKEQEETINELRVNLSEKETEISTIQKQLEAINDKLQNKIQEIYEKEEQFNIKQISEVQEKVNELKQFKEHRKAKDSALQSIESKMLELTNRLQESQEEIQIMIKEKEEMKRVQEALQIERDQLKENTKEIVAKMKESQEKEYQFLKMTAVNETQEKMCEIEHLKEQFETQKLNLENIETENIRLTQILHENLEEMRSVTKERDDLRSVEETLKVERDQLKENLRETITRDLEKQEELKIVHMHLKEHQETIDKLRGIVSEKTNEISNMQKDLEHSNDALKAQDLKIQEELRIAHMHLKEQQETIDKLRGIVSEKTDKLSNMQKDLENSNAKLQEKIQELKANEHQLITLKKDVNETQKKVSEMEQLKKQIKDQSLTLSKLEIENLNLAQKLHENLEEMKSVMKERDNLRRVEETLKLERDQLKESLQETKARDLEIQQELKTARMLSKEHKETVDKLREKISEKTIQISDIQKDLDKSKDELQKKIQELQKKELQLLRVKEDVNMSHKKINEMEQLKKQFEAQNLSMQSVRMDNFQLTKKLHESLEEIRIVAKERDELRRIKESLKMERDQFIATLREMIARDRQNHQVKPEKRLLSDGQQHLTESLREKCSRIKELLKRYSEMDDHYECLNRLSLDLEKEIEFQKELSMRVKANLSLPYLQTKHIEKLFTANQRCSMEFHRIMKKLKYVLSYVTKIKEEQHESINKFEMDFIDEVEKQKELLIKIQHLQQDCDVPSRELRDLKLNQNMDLHIEEILKDFSESEFPSIKTEFQQVLSNRKEMTQFLEEWLNTRFDIEKLKNGIQKENDRICQVNNFFNNRIIAIMNESTEFEERSATISKEWEQDLKSLKEKNEKLFKNYQTLKTSLASGAQVNPTTQDNKNPHVTSRATQLTTEKIRELENSLHEAKESAMHKESKIIKMQKELEVTNDIIAKLQAKVHESNKCLEKTKETIQVLQDKVALGAKPYKEEIEDLKMKLVKIDLEKMKNAKEFEKEISATKATVEYQKEVIRLLRENLRRSQQAQDTSVISEHTDPQPSNKPLTCGGGSGIVQNTKALILKSEHIRLEKEISKLKQQNEQLIKQKNELLSNNQHLSNEVKTWKERTLKREAHKQVTCENSPKSPKVTGTASKKKQITPSQCKERNLQDPVPKESPKSCFFDSRSKSLPSPHPVRYFDNSSLGLCPEVQNAGAESVDSQPGPWHASSGKDVPECKTQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
91
Pig
89
Bovine
73
Rabbit
73
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02224 As Substrate

Site PTM Type Enzyme
K45 Ubiquitination
S130 Phosphorylation
Y131 Phosphorylation
T146 Phosphorylation
Y163 Phosphorylation
Y173 Phosphorylation
Y191 Phosphorylation
T194 Phosphorylation
K227 Ubiquitination
K253 Ubiquitination
K271 Ubiquitination
S273 Phosphorylation
Y283 Phosphorylation
K301 Ubiquitination
S312 Phosphorylation
T316 Phosphorylation
Y328 Phosphorylation
Y334 Phosphorylation
K346 Ubiquitination
Y348 Phosphorylation
K356 Acetylation
K357 Ubiquitination
K373 Ubiquitination
K383 Ubiquitination
K388 Ubiquitination
K393 Ubiquitination
T422 Phosphorylation O14965 (AURKA) , Q96GD4 (AURKB)
K432 Ubiquitination
K449 Ubiquitination
S454 Phosphorylation
K531 Ubiquitination
K564 Ubiquitination
K580 Ubiquitination
K602 Ubiquitination
S611 Phosphorylation
S613 Phosphorylation
K638 Ubiquitination
S692 Phosphorylation
T698 Phosphorylation
S702 Phosphorylation
K707 Ubiquitination
K779 Ubiquitination
S901 Phosphorylation
T982 Phosphorylation
S985 Phosphorylation
K986 Ubiquitination
K1096 Ubiquitination
K1105 Ubiquitination
K1110 Ubiquitination
T1179 Phosphorylation
K1187 Ubiquitination
Y1192 Phosphorylation
S1211 Phosphorylation
K1261 Ubiquitination
T1268 Phosphorylation
T1316 Phosphorylation
T1321 Phosphorylation
K1336 Ubiquitination
S1340 Phosphorylation
K1369 Ubiquitination
Y1628 Phosphorylation
K1657 Ubiquitination
K1706 Ubiquitination
K1739 Ubiquitination
T1801 Phosphorylation
S1814 Phosphorylation
S1886 Phosphorylation
K1937 Ubiquitination
T1951 Phosphorylation
S2083 Phosphorylation
K2126 Ubiquitination
S2143 Phosphorylation
K2230 Ubiquitination
K2284 Ubiquitination
K2350 Ubiquitination
S2352 Phosphorylation
T2363 Phosphorylation
S2389 Phosphorylation
K2451 Ubiquitination
S2581 Phosphorylation
T2601 Phosphorylation
S2605 Phosphorylation P28482 (MAPK1)
S2608 Phosphorylation P28482 (MAPK1)
T2612 Phosphorylation
S2616 Phosphorylation
T2622 Phosphorylation
S2639 Phosphorylation P28482 (MAPK1)
K2641 Ubiquitination
S2647 Phosphorylation
S2649 Phosphorylation
S2651 Phosphorylation
S2654 Phosphorylation P28482 (MAPK1)
S2664 Phosphorylation

Research Backgrounds

Function:

Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized. Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity).

PTMs:

The C-terminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity).

Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore.

Subcellular Location:

Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Centromere.
Note: Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division (By similarity). Recruited to the kinetochore in a SEPT7, CENPQ and TRAPPC12-dependent manner (PubMed:18460473, PubMed:25918224, PubMed:25395579). Recruited to the pericentromeric/centromeric regions of the chromosome in a CTCF-dependent manner (PubMed:26321640).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer. Interacts with CENPF. Interacts with BUB1B. Interacts with SEPT7. Interacts with KIF18A. Interacts with PRC1. Interacts with NUF2; this interaction determines kinetochore localization. Interacts with SKAP; this interaction greatly favors SKAP binding to microtubules. Interacts with TRAPPC12. Interacts with CTCF.

Family&Domains:

The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

References

1). Reduced expression of CENP-E contributes to the development of hepatocellular carcinoma and is associated with adverse clinical features. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 31881483) [IF=6.9]

Application: IHC    Species: mouse    Sample: tumor

Fig. 5.| Down regulation of CENP-E in HCC cells promoted tumor growth of in vivo.(C) Tumor sections were subjected to IHC staining, showing the increased expression of GPC-3 but no difference of ARG-1 expression in CENP-E-low-expressing tumors versus control tumors (ARG-1:P > 0.05, GPC-3:P < 0.05). 40× magnifications, scale bar 100 μm. Statistical significance was tested by Student’s t-test.

Application: WB    Species: mouse    Sample: tumor

Fig. 5.| Down regulation of CENP-E in HCC cells promoted tumor growth of in vivo.(A) Down-regulation of CENP-E expression in human HCC derived cell line SMMC7721 cells promoted the growth of HCC in vivo (P < 0.05). (B) Tumors of HCC in vivo were collected for western blotting.
2). Primary microcephaly gene CENPE is a novel biomarker and potential therapeutic target for non-WNT/non-SHH medulloblastoma. Frontiers in Immunology, 2023 (PubMed: 37593739) [IF=5.7]

Application: WB    Species: Human    Sample:

Figure 9 CENPE regulates the cell cycle and p53 pathway of non-WNT/non-SHH MB. (A–E) Western blot showed that the protein level of CENPE, P53, P21 and CDK1 by the knockdown of CENPE (mean ± SD, n = 3). (F–H) Flow cytometry was used to detect the cell cycle (mean ± SD, n = 3).

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