GLIS1 Antibody | Affinity Biosciences

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GLIS1 Antibody - Western blot analysis of GLIS1 using HT29 whole lysates.

Product:	GLIS1 Antibody
Catalog:	DF7748
Description:	Rabbit polyclonal antibody to GLIS1
Application:	WB
Reactivity:	Human
Prediction:	Pig, Horse, Rabbit, Dog
Mol.Wt.:	65 kDa; 66kD(Calculated).
Uniprot:	Q8NBF1
RRID:	AB_2841214

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(90%), Horse(80%), Rabbit(90%), Dog(90%)

Clonality:

Polyclonal

Specificity:

GLIS1 Antibody detects endogenous levels of total GLIS1.

RRID:

AB_2841214
Cite Format: Affinity Biosciences Cat# DF7748, RRID:AB_2841214.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ36155|; GLI similar 1; GLI-similar 1; Gli5 like; GLIS 1; GLIS family zinc finger 1; Glis1; Glis1 like; GLIS1_HUMAN; Zinc finger protein GLIS1;

Immunogens

Immunogen:

Uniprot:

Q8NBF1(GLIS1_HUMAN) >>Visit HPA database.

Gene(ID):

GLIS1(148979)

Sequence:

Q8NBF1 GLIS1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEARTSLSAHCRGPLATGLHPDLDLPGRSLATPAPSCYLLGSEPSSGLGLQPETHLPEGSLKRCCVLGLPPTSPASSSPCASSDVTSIIRSSQTSLVTCVNGLRSPPLTGDLGGPSKRARPGPASTDSHEGSLQLEACRKASFLKQEPADEFSELFGPHQQGLPPPYPLSQLPPGPSLGGLGLGLAGRVVAGRQACRWVDCCAAYEQQEELVRHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLKIHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKEQQVRKKLHAGPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Rabbit

Horse

Sheep

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NBF1 As Substrate

Q8NBF1

Site	PTM Type	Source
S106	Phosphorylation	Uniprot
S143	Phosphorylation	Uniprot
Y240	Phosphorylation	Uniprot
S460	Phosphorylation	Uniprot
S464	Phosphorylation	Uniprot

Research Backgrounds

Q8NBF1_GLIS1_HUMAN

Function:

Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3' (By similarity). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells. Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (By similarity).

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with KLF4. Interacts with POU5F1 and/or POU5F1B. Interacts with SOX2.

Family&Domains:

Belongs to the GLI C2H2-type zinc-finger protein family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

GLIS1 Antibody - #DF7748