Nestin Antibody - #DF7754

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Product: Nestin Antibody
Catalog: DF7754
Description: Rabbit polyclonal antibody to Nestin
Application: WB IHC IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 160kD,210kD; 177kD(Calculated).
Uniprot: P48681
RRID: AB_2841220

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(93%), Dog(86%)
Clonality:
Polyclonal
Specificity:
Nestin Antibody detects endogenous levels of total Nestin.
RRID:
AB_2841220
Cite Format: Affinity Biosciences Cat# DF7754, RRID:AB_2841220.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin;

Immunogens

Immunogen:

A synthesized peptide derived from human Nestin, corresponding to a region within C-terminal amino acids.

Uniprot:

P48681(NEST_HUMAN) >>Visit HPA database.

Gene(ID):
NES(10763)
Expression:
P48681 NEST_HUMAN:

CNS stem cells.

Sequence:
MEGCMGEESFQMWELNRRLEAYLARVKALEEQNELLSAELGGLRAQSADTSWRAHADDELAALRALVDQRWREKHAAEVARDNLAEELEGVAGRCQQLRLARERTTEEVARNRRAVEAEKCARAWLSSQVAELERELEALRVAHEEERVGLNAQAACAPRCPAPPRGPPAPAPEVEELARRLGEAWRGAVRGYQERVAHMETSLGQARERLGRAVQGAREGRLELQQLQAERGGLLERRAALEQRLEGRWQERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGGGSKTSLSFQDPKLELQFPRTPEGRRLGSLLPVLSPTSLPSPLPATLETPVPAFLKNQEFLQARTPTLASTPIPPTPQAPSPAVDAEIRAQDAPLSLLQTQGGRKQAPEPLRAEARVAIPASVLPGPEEPGGQRQEASTGQSPEDHASLAPPLSPDHSSLEAKDGESGGSRVFSICRGEGEGQIWGLVEKETAIEGKVVSSLQQEIWEEEDLNRKEIQDSQVPLEKETLKSLGEEIQESLKTLENQSHETLERENQECPRSLEEDLETLKSLEKENKELLKDVEVVRPLEKEAVGQLKPTGKEDTQTLQSLQKENQELMKSLEGNLETFLFPGTENQELVSSLQENLESLTALEKENQEPLRSPEVGDEEALRPLTKENQEPLRSLEDENKEAFRSLEKENQEPLKTLEEEDQSIVRPLETENHKSLRSLEEQDQETLRTLEKETQQRRRSLGEQDQMTLRPPEKVDLEPLKSLDQEIARPLENENQEFLKSLKEESVEAVKSLETEILESLKSAGQENLETLKSPETQAPLWTPEEINQGAMNPLEKEIQEPLESVEVNQETFRLLEEENQESLRSLGAWNLENLRSPEEVDKESQRNLEEEENLGKGEYQESLRSLEEEGQELPQSADVQRWEDTVEKDQELAQESPPGMAGVENEDEAELNLREQDGFTGKEEVVEQGELNATEEVWIPGEGHPESPEPKEQRGLVEGASVKGGAEGLQDPEGQSQQVGAPGLQAPQGLPEAIEPLVEDDVAPGGDQASPEVMLGSEPAMGESAAGAEPGPGQGVGGLGDPGHLTREEVMEPPLEEESLEAKRVQGLEGPRKDLEEAGGLGTEFSELPGKSRDPWEPPREGREESEAEAPRGAEEAFPAETLGHTGSDAPSPWPLGSEEAEEDVPPVLVSPSPTYTPILEDAPGPQPQAEGSQEASWGVQGRAEALGKVESEQEELGSGEIPEGPQEEGEESREESEEDELGETLPDSTPLGFYLRSPTSPRWDPTGEQRPPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
93
Dog
86
Pig
79
Horse
79
Bovine
79
Sheep
79
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

PTMs:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).

Tissue Specificity:

CNS stem cells.

Family&Domains:

Belongs to the intermediate filament family.

References

1). Biomimetic Multichannel Silk Nerve Conduits With Multicellular Spatiotemporal Distributions for Spinal Cord Injury Repair. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39268784) [IF=27.4]
2). An anti-inflammatory and neuroprotective biomimetic nanoplatform for repairing spinal cord injury. Bioactive Materials, 2022 (PubMed: 35845318) [IF=18.9]
3). Neurogenesis promoted by the CD200/CD200R signaling pathway following treadmill exercise enhances post-stroke functional recovery in rats. BRAIN BEHAVIOR AND IMMUNITY, 2019 (PubMed: 31513876) [IF=8.8]

Application: IF/ICC    Species: rat    Sample: astrocytes

Fig. 5.| The expression of CD200 and CD200R was up-regulated induced by treadmill exercise after stroke in rats.The effect of treadmill exercise on the protein levels of CD200 and CD200R in the ischemic cortex (A-C) and hippocampus (D-F) at 7 and 28 days after tMCAO. CD200R staining shows protein expression at 7 and 28 days after treadmill exercise post-stroke (G). CD200R colocalizes with activated microglia (Iba1+cells, white arrows), not colocalizes with neural stem cells (Nestin+ cells) and astrocytes (GFAP+ cells), Scale bar: 50 μm. N = 4. Results are showed as means ± SEM. # P < 0.05, ## P < 0.01, ### P < 0.001 versus the Sham group. *P < 0.05, ** P < 0.01, *** P < 0.001 versus the tMCAO group.
4). Mesenchymal stem cells overexpressing neuropeptide S promote the recovery of rats with spinal cord injury by activating the PI3K/AKT/GSK3β signaling pathway. Stem cell research & therapy, 2025 (PubMed: 40022159) [IF=7.5]

Application: IF/ICC    Species: Rat    Sample:

Fig. 3 Cell culture and identification of stem cells derived from the nasal mucosa of rats. (a) Immunofluorescence for positive markers (CD44, vimentin, Sox2, and Nestin) on the surfaces of EMSCs and NPS-EMSCs. Scale bar = 100 μm. (b) Phase-contrast microscopy image of EMSCs from primary cultures to the third passage and NPS overexpressing EMSCs. Scale bar = 100 μm; Alkaline phosphatase staining indicates that mesenchymal stem cells derived from the nasal mucosa have osteogenic differentiation ability, and Oil Red O staining reveals that EMSCs have adipose differentiation ability. Scale bar = 20 μm. (c) AM/PI double-staining fluorescence images at 1, 3, and 5 days, with green indicating live cells and red indicating dead cells. Scale bar = 100 μm. (d) Scanning electron microscopy image of the hydrogel scaffold demonstrating its porous structure
5). Effects of RO27-3225 on neurogenesis, PDGFRβ+ cells and neuroinflammation after cerebral infarction. International Immunopharmacology, 2020 (PubMed: 32058930) [IF=4.8]

Application: IF/ICC    Species: Human    Sample: neonatal neural stem cells

Fig. 1. RO27-3225 promotes stem cell proliferation in the SVZ after cerebral infarction. (A) Immunofluorescence staining of neonatal neural stem cells (Nestin+/ BrdU+) in the SVZ on day 7 after tMCAO. (B) The quantification results showed that the mice belonging to the tMCAO + saline group had more Nestin+/BrdU+ cells in the SVZ on day 7 after tMCAO than the mice belonging to the sham + saline group, and RO27-3225 strengthened this increase (*p < 0.05 vs. the corresponding sham group; #p < 0.05 vs. the tMCAO + RO27-3225 group; n = 6/group). The data are shown as the means ± SDs.
6). Repeated Ketamine Anesthesia during the Neonatal Period Impairs Hippocampal Neurogenesis and Long-Term Neurocognitive Function by Inhibiting Mfn2-Mediated Mitochondrial Fusion in Neural Stem Cells. Molecular neurobiology, 2024 (PubMed: 38200350) [IF=4.6]
7). miR-872-5p/FOXO3a/Wnt signaling feed-forward loop promotes proliferation of endogenous neural stem cells after spinal cord ischemia–reperfusion injury in rats. The FASEB Journal, 2023 (PubMed: 36607643) [IF=4.4]
8). Protective effect of bone morphogenetic protein-7 induced differentiation of bone marrow mesenchymal stem cells in rat with acute spinal cord injury. Functional & integrative genomics, 2023 (PubMed: 36849554) [IF=3.9]
9). Astragaloside IV Treats Parkinson's Disease by Regulating the Proliferation and Differentiation of NSCs through the SHH-Nurr1 Pathway. Stem cells international, 2024 (PubMed: 39257865) [IF=3.8]
10). Depletion of MicroRNA-100-5p Promotes Osteogenesis Via Lysine(K)-Specific Demethylase 6B. Tissue engineering. Part A, 2024 (PubMed: 39718900) [IF=3.5]
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