MRRF Antibody - #AF0770

(3)  
Product: MRRF Antibody
Catalog: AF0770
Description: Rabbit polyclonal antibody to MRRF
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 28kDa; 29kD(Calculated).
Uniprot: Q96E11
RRID: AB_2834403

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
MRRF Antibody detects endogenous levels of total MRRF.
RRID:
AB_2834403
Cite Format: Affinity Biosciences Cat# AF0770, RRID:AB_2834403.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2400002D02Rik; MGC93722; mitochondrial; mitochondrial ribosome recycling factor; MRFF; Mrrf; MTRRF; OTTHUMP00000022037; OTTMUSP00000013027; Ribosome recycling factor, mitochondrial; Ribosome recycling factor, mitochondrial precursor; Ribosome-recycling factor; Ribosome-releasing factor; Ribosome-releasing factor, mitochondrial; RP23-324A16.1; RRF; RRFM_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q96E11(RRFM_HUMAN) >>Visit HPA database.

Gene(ID):
MRRF(92399)
Description:
MRRF Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. Belongs to the RRF family. 7 isoforms of the human protein are produced by alternative splicing.
Sequence:
MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
92
Xenopus
67
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96E11 As Substrate

Site PTM Type Enzyme
S112 Phosphorylation
S115 Phosphorylation
T225 Phosphorylation
T245 Phosphorylation

Research Backgrounds

Function:

Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity).

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the RRF family.

Restrictive clause

 

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