Product: Sall4 Antibody
Catalog: DF7757
Description: Rabbit polyclonal antibody to Sall4
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Dog
Mol.Wt.: 140kD, 60kD(isoform); 112kD(Calculated).
Uniprot: Q9UJQ4
RRID: AB_2841223

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Dog(83%)
Clonality:
Polyclonal
Specificity:
Sall4 Antibody detects endogenous levels of total Sall4.
RRID:
AB_2841223
Cite Format: Affinity Biosciences Cat# DF7757, RRID:AB_2841223.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AA407717; AL022809; AW536104; C330011P20Rik; C78083; C78563; dJ1112F19.1; DRRS; HSAL4; Sal like 4 (Drosophila); Sal like 4; Sal like Protein 4; Sal-like protein 4; Sall4; SALL4_HUMAN; Spalt like transcription factor 4; Tex20; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UJQ4 SALL4_HUMAN:

Expressed in testis. Constitutively expressed in acute myeloid leukemia (AML).

Sequence:
MSRRKQAKPQHINSEEDQGEQQPQQQTPEFADAAPAAPAAGELGAPVNHPGNDEVASEDEATVKRLRREETHVCEKCCAEFFSISEFLEHKKNCTKNPPVLIMNDSEGPVPSEDFSGAVLSHQPTSPGSKDCHRENGGSSEDMKEKPDAESVVYLKTETALPPTPQDISYLAKGKVANTNVTLQALRGTKVAVNQRSADALPAPVPGANSIPWVLEQILCLQQQQLQQIQLTEQIRIQVNMWASHALHSSGAGADTLKTLGSHMSQQVSAAVALLSQKAGSQGLSLDALKQAKLPHANIPSATSSLSPGLAPFTLKPDGTRVLPNVMSRLPSALLPQAPGSVLFQSPFSTVALDTSKKGKGKPPNISAVDVKPKDEAALYKHKCKYCSKVFGTDSSLQIHLRSHTGERPFVCSVCGHRFTTKGNLKVHFHRHPQVKANPQLFAEFQDKVAAGNGIPYALSVPDPIDEPSLSLDSKPVLVTTSVGLPQNLSSGTNPKDLTGGSLPGDLQPGPSPESEGGPTLPGVGPNYNSPRAGGFQGSGTPEPGSETLKLQQLVENIDKATTDPNECLICHRVLSCQSSLKMHYRTHTGERPFQCKICGRAFSTKGNLKTHLGVHRTNTSIKTQHSCPICQKKFTNAVMLQQHIRMHMGGQIPNTPLPENPCDFTGSEPMTVGENGSTGAICHDDVIESIDVEEVSSQEAPSSSSKVPTPLPSIHSASPTLGFAMMASLDAPGKVGPAPFNLQRQGSRENGSVESDGLTNDSSSLMGDQEYQSRSPDILETTSFQALSPANSQAESIKSKSPDAGSKAESSENSRTEMEGRSSLPSTFIRAPPTYVKVEVPGTFVGPSTLSPGMTPLLAAQPRRQAKQHGCTRCGKNFSSASALQIHERTHTGEKPFVCNICGRAFTTKGNLKVHYMTHGANNNSARRGRKLAIENTMALLGTDGKRVSEIFPKEILAPSVNVDPVVWNQYTSMLNGGLAVKTNEISVIQSGGVPTLPVSLGATSVVNNATVSKMDGSQSGISADVEKPSATDGVPKHQFPHFLEENKIAVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Pig
75
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UJQ4 As Substrate

Site PTM Type Enzyme
S57 Phosphorylation
S116 Phosphorylation
S121 Phosphorylation
K173 Acetylation
K175 Sumoylation
S307 Phosphorylation
T393 Phosphorylation
S395 Phosphorylation
S530 Phosphorylation
T541 Phosphorylation
S576 Phosphorylation
S748 Phosphorylation
S753 Phosphorylation
S765 Phosphorylation
S776 Phosphorylation
S789 Phosphorylation
S793 Phosphorylation
S824 Phosphorylation
S852 Phosphorylation
T856 Phosphorylation
T873 Phosphorylation
S880 Phosphorylation
S881 Phosphorylation
S883 Phosphorylation
T891 Phosphorylation
T893 Phosphorylation
S1019 Phosphorylation

Research Backgrounds

Function:

Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.

PTMs:

Isoform SALL4B exists primarily as a ubiquitinated form.

Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testis. Constitutively expressed in acute myeloid leukemia (AML).

Subunit Structure:

Interacts with NANOG (By similarity). Interacts with POU5F1/OCT4. Interacts with BEND3.

Family&Domains:

Belongs to the sal C2H2-type zinc-finger protein family.

Restrictive clause

 

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For Research Use Only.
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