Product: BMP9 Antibody
Catalog: DF7758
Description: Rabbit polyclonal antibody to BMP9
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Rabbit
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: Q9UK05
RRID: AB_2841224

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(89%), Sheep(89%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
BMP9 Antibody detects endogenous levels of total BMP9.
RRID:
AB_2841224
Cite Format: Affinity Biosciences Cat# DF7758, RRID:AB_2841224.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BMP 9; Bone morphogenetic protein 9; GDF 2; GDF2; growth differentiation factor 2; Growth/differentiation factor 2; HHT5;

Immunogens

Immunogen:

A synthesized peptide derived from human BMP9, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
Q9UK05 GDF2_HUMAN:

Detected in blood plasma (at protein level).

Sequence:
MCPGALWVALPLLSLLAGSLQGKPLQSWGRGSAGGNAHSPLGVPGGGLPEHTFNLKMFLENVKVDFLRSLNLSGVPSQDKTRVEPPQYMIDLYNRYTSDKSTTPASNIVRSFSMEDAISITATEDFPFQKHILLFNISIPRHEQITRAELRLYVSCQNHVDPSHDLKGSVVIYDVLDGTDAWDSATETKTFLVSQDIQDEGWETLEVSSAVKRWVRSDSTKSKNKLEVTVESHRKGCDTLDISVPPGSRNLPFFVVFSNDHSSGTKETRLELREMISHEQESVLKKLSKDGSTEAGESSHEEDTDGHVAAGSTLARRKRSAGAGSHCQKTSLRVNFEDIGWDSWIIAPKEYEAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISVLYKDDMGVPTLKYHYEGMSVAECGCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
89
Sheep
89
Pig
78
Dog
78
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

PTMs:

A reversible disulfide bond can be formed between the two subunits in the homodimer; this has no effect on GDF2 activity.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in blood plasma (at protein level).

Family&Domains:

Belongs to the TGF-beta family.

References

1). ITE promotes hypoxia-induced transdifferentiation of human pulmonary arterial endothelial cells possibly by activating transforming growth factor-β/Smads and MAPK/ERK pathways. JOURNAL OF CELLULAR BIOCHEMISTRY, 2019 (PubMed: 31297875) [IF=3.0]

Application: WB    Species: human    Sample: HPAECs

FIGURE 6 |Effects of ITE on the expression of TGF‐β1, BMP2 and BMP9 in HPAECs induced by hypoxia. HPAECs were cultured under the conditions of normoxia, normoxia + ITE, hypoxia and hypoxia + ITE, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001. BMP, bone morphogenetic protein; HPAEC, human pulmonary arterial endothelial cell; ITE, 2‐(1′H‐indole‐3′‐carbonyl)‐thiazole‐4‐carboxylicacid methyl ester; TGF‐β, transforming growth factor‐β

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.