BMP9 Antibody - #DF7758

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Product: BMP9 Antibody
Catalog: DF7758
Description: Rabbit polyclonal antibody to BMP9
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Rabbit
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: Q9UK05
RRID: AB_2841224

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(89%), Sheep(89%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
BMP9 Antibody detects endogenous levels of total BMP9.
RRID:
AB_2841224
Cite Format: Affinity Biosciences Cat# DF7758, RRID:AB_2841224.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BMP 9; Bone morphogenetic protein 9; GDF 2; GDF2; growth differentiation factor 2; Growth/differentiation factor 2; HHT5;

Immunogens

Immunogen:
Uniprot:

Q9UK05(GDF2_HUMAN) >>Visit HPA database.

Gene(ID):
GDF2(2658)
Expression:
Q9UK05 GDF2_HUMAN:

Detected in blood plasma (at protein level).

Sequence:
MCPGALWVALPLLSLLAGSLQGKPLQSWGRGSAGGNAHSPLGVPGGGLPEHTFNLKMFLENVKVDFLRSLNLSGVPSQDKTRVEPPQYMIDLYNRYTSDKSTTPASNIVRSFSMEDAISITATEDFPFQKHILLFNISIPRHEQITRAELRLYVSCQNHVDPSHDLKGSVVIYDVLDGTDAWDSATETKTFLVSQDIQDEGWETLEVSSAVKRWVRSDSTKSKNKLEVTVESHRKGCDTLDISVPPGSRNLPFFVVFSNDHSSGTKETRLELREMISHEQESVLKKLSKDGSTEAGESSHEEDTDGHVAAGSTLARRKRSAGAGSHCQKTSLRVNFEDIGWDSWIIAPKEYEAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISVLYKDDMGVPTLKYHYEGMSVAECGCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
89
Sheep
89
Pig
78
Dog
78
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UK05 As Substrate

Site PTM Type Enzyme
S69 Phosphorylation
T102 Phosphorylation
T103 Phosphorylation
S263 Phosphorylation
S277 Phosphorylation
T293 Phosphorylation
S312 Phosphorylation
S399 Phosphorylation
S402 Phosphorylation
Y405 Phosphorylation

Research Backgrounds

Function:

Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

PTMs:

A reversible disulfide bond can be formed between the two subunits in the homodimer; this has no effect on GDF2 activity.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in blood plasma (at protein level).

Subunit Structure:

Homodimer; disulfide-linked. Detected in extracellular fluid as mature homodimer, and in complex with its propeptide. Interacts with ACVRL1, BMPR2 and ACVR2B with high affinity (in vitro). Identified in a complex with ACVRL1 and ACVR2B. Has ten times lower affinity for ACVR2A (in vitro). Interacts with ENG, forming a heterotetramer with a 2:2 stoichiometry. Can form a heteromeric complex with ENG and ACVRL1.

Family&Domains:

Belongs to the TGF-beta family.

References

1). ITE promotes hypoxia-induced transdifferentiation of human pulmonary arterial endothelial cells possibly by activating transforming growth factor-β/Smads and MAPK/ERK pathways. JOURNAL OF CELLULAR BIOCHEMISTRY, 2019 (PubMed: 31297875) [IF=3.0]

Application: WB    Species: human    Sample: HPAECs

FIGURE 6 |Effects of ITE on the expression of TGF‐β1, BMP2 and BMP9 in HPAECs induced by hypoxia. HPAECs were cultured under the conditions of normoxia, normoxia + ITE, hypoxia and hypoxia + ITE, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001. BMP, bone morphogenetic protein; HPAEC, human pulmonary arterial endothelial cell; ITE, 2‐(1′H‐indole‐3′‐carbonyl)‐thiazole‐4‐carboxylicacid methyl ester; TGF‐β, transforming growth factor‐β

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