IGFBP2 Antibody - #DF7765

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Product: IGFBP2 Antibody
Catalog: DF7765
Description: Rabbit polyclonal antibody to IGFBP2
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: P18065
RRID: AB_2841231

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
IGFBP2 Antibody detects endogenous levels of total IGFBP2.
RRID:
AB_2841231
Cite Format: Affinity Biosciences Cat# DF7765, RRID:AB_2841231.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BP 2; BP2; IBP 2; IBP-2; IBP2; IBP2_HUMAN; IGF binding protein 2; IGF BP53; IGF-binding protein 2; IGFBP 2; IGFBP-2; IGFBP2; IGFBP53; Insulin like growth factor binding protein 2 36kDa; Insulin like growth factor binding protein 2; Insulin like growth factor-binding protein 2 precursor; Insulin-like growth factor-binding protein 2;

Immunogens

Immunogen:
Uniprot:

P18065(IBP2_HUMAN) >>Visit HPA database.

Gene(ID):
IGFBP2(3485)
Sequence:
MLPRVGCPALPLPPPPLLPLLLLLLGASGGGGGARAEVLFRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
86
Chicken
86
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18065 As Substrate

Site PTM Type Enzyme
S142 Phosphorylation
T196 Phosphorylation
K207 Acetylation
K292 Ubiquitination
T299 Phosphorylation

Research Backgrounds

Function:

Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

PTMs:

O-glycosylated.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds IGF2 more than IGF1.

Family&Domains:

The C-terminus is required for IGF-binding and growth inhibition.

References

1). Dissecting the single-cell transcriptome network underlying esophagus non-malignant tissues and esophageal squamous cell carcinoma. eBioMedicine, 2021 (PubMed: 34192657) [IF=9.7]

Application: IF/ICC    Species: Rabbit    Sample: epithelial cells

Fig. 3. The single-cell transcriptomes of epithelial cells in non-malignant and malignant esophagus.(a) The TSNE plot and overview of epithelial cells by the cell type and origin of the cells. (b) Canonical cell markers were used to label epithelial subtypes as represented in the TSNE plot. (c) The TSNE plot of epithelial cells by the CNV’s levels of the cells. (d) Heatmap showing the expression of marker genes in malignant and non-malignant epithelial cells. (e) Violin plots showing the marker genes for each epithelial cell type. (f) Immunofluorescence staining of tumor (n = 8) (up) / normal (n = 8) (down) tissue-specific genes in ESCC and non-malignant tissues. Comparison of non-malignant and malig- nant esophagus was performed by Wilcoxon test. (g) Pseudotime analysis for malignant and non-malignant epithelial cells. (h) Differences in pathway activities scored per cell by GSVA between malignant and non-malignant epithelial cells. The scores of pathways are normalized.
2). Early pregnancy human decidua gamma/delta T cells exhibit tissue resident and specific functional characteristics. Molecular Human Reproduction, 2022 (PubMed: 35758607) [IF=3.6]

Application: IF/ICC    Species: Human    Sample: γδ T+ Cells

Figure 5.Identification of growth-factor secretion in human γδ T+ Cells. (A) qRT-PCR results from eight selected genes using RNA-sequencing. (B) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from early decidua (n = 5). (C) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from term decidua (n = 5). (D) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from peripheral blood mononuclear cells (PBMCs) (n = 5). (E) Immunofluorescence analysis of growth-factor expression in sorted γδ T cells from recurrent spontaneous abortion (RSA) patients (n = 5; scale bar, 50 μm). (F) qRT-PCR results regarding growth factors in sorted γδ T cells from RSA patients (n = 5). P-value was calculated by unpaired Student’s t-test.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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