TNFSF4 Antibody - #DF7816

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Product: TNFSF4 Antibody
Catalog: DF7816
Description: Rabbit polyclonal antibody to TNFSF4
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Monkey
Prediction: Rabbit
Mol.Wt.: 21 kDa; 21kD(Calculated).
Uniprot: P23510
RRID: AB_2841265

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Rabbit(90%)
Clonality:
Polyclonal
Specificity:
TNFSF4 Antibody detects endogenous levels of total TNFSF4.
RRID:
AB_2841265
Cite Format: Affinity Biosciences Cat# DF7816, RRID:AB_2841265.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 134L; CD 252; CD134; CD134 ligand; CD134L; CD252; CD252 antigen; glycoprotein 34 kd; Glycoprotein Gp34; GP34; OX-40L; OX40 antigen ligand; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; TNFL4_HUMAN; Tnfsf4; Tumor necrosis factor (ligand) superfamily member 4; Tumor necrosis factor ligand superfamily member 4; TXGP1;

Immunogens

Immunogen:

A synthesized peptide derived from human TNFSF4, corresponding to a region within N-terminal amino acids.

Uniprot:

P23510(TNFL4_HUMAN) >>Visit HPA database.

Gene(ID):
TNFSF4(7292)
Sequence:
MERVQPLEENVGNAARPRFERNKLLLVASVIQGLGLLLCFTYICLHFSALQVSHRYPRIQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGFYLISLKGYFSQEVNISLHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVYLNVTTDNTSLDDFHVNGGELILIHQNPGEFCVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Horse
50
Dog
50
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

Subcellular Location:

Membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the tumor necrosis factor family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

References

1). MiR-125b-5p ameliorates ox-LDL-induced vascular endothelial cell dysfunction by negatively regulating TNFSF4/TLR4/NF-κB signaling. BMC biotechnology, 2025 (PubMed: 39856662) [IF=3.5]

Application: WB    Species: human    Sample: HUVECs

Fig. 1 Expression levels of miR-125b-5p, TNFSF4 and TLR4/NF-κB signaling factors in an in vitro AS model. HUVECs were exposed to diverse concentrations (25, 50, 100 µg/ml) of ox-LDL for 24 h. (A) Cell viability of HUVECs was determined using CCK-8 assay. (B-C) The expression of miR-125b-5p and TNFSF4 mRNA was measured using quantitative real-time PCR analysis in HUVECs. (D) The protein levels of TNFSF4, TLR4, p-p65, p65, p-IκBα and IκBα were visualized and quantified via western blot analysis in HUVECs. All experiments were carried out three times with three repetitions. *p 

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