HMGB3 Antibody - #DF7825
| Product: | HMGB3 Antibody |
| Catalog: | DF7825 |
| Description: | Rabbit polyclonal antibody to HMGB3 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 23 kDa; 23kD(Calculated). |
| Uniprot: | O15347 |
| RRID: | AB_2841269 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7825, RRID:AB_2841269.
Fold/Unfold
chromosomal protein, Nonhistone, HMG4; High mobility group (nonhistone chromosomal) protein 4; High mobility group box 3; High mobility group protein 2a; High mobility group protein 4; High mobility group protein B3; High mobility group protein HMG4; HMG 4; HMG-2a; HMG-4; HMG2A; HMGB 3; Hmgb3; HMGB3_HUMAN; MGC90319; Non histone chromosomal protein; Nonhistone chromosomal protein HMG4;
Immunogens
- O15347 HMGB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKGDPKKPKGKMSAYAFFVQTCREEHKKKNPEVPVNFAEFSKKCSERWKTMSGKEKSKFDEMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKRPPSGFFLFCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLNDSEKQPYITKAAKLKEKYEKDVADYKSKGKFDGAKGPAKVARKKVEEEDEEEEEEEEEEEEEEDE
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O15347 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K12 | Acetylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K59 | Acetylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| Y71 | Phosphorylation | Uniprot | |
| Y78 | Phosphorylation | Uniprot | |
| K82 | Acetylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K139 | Acetylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K145 | Acetylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K152 | Acetylation | Uniprot | |
| K161 | Acetylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K165 | Acetylation | Uniprot | |
| K170 | Acetylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K179 | Methylation | Uniprot |
Research Backgrounds
Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).
Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so).
Nucleus. Chromosome. Cytoplasm.
Expressed predominantly in placenta.
Belongs to the HMGB family.
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