Band 3/AE 1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of SLC4A1 using RAW264.

Product:	Band 3/AE 1 Antibody
Catalog:	DF7843
Description:	Rabbit polyclonal antibody to Band 3/AE 1
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Horse, Rabbit, Dog
Mol.Wt.:	102 kDa; 102kD(Calculated).
Uniprot:	P02730
RRID:	AB_2841281

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Horse(82%), Rabbit(92%), Dog(83%)

Clonality:

Polyclonal

Specificity:

Band 3/AE 1 Antibody detects endogenous levels of total Band 3/AE 1.

RRID:

AB_2841281
Cite Format: Affinity Biosciences Cat# DF7843, RRID:AB_2841281.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AE 1; AE1; Anion exchange protein 1; Anion exchanger 1; B3AT_HUMAN; Band 3 anion transport protein; BND3; CD233; DI; Diego blood group; EMPB3; EPB3; Erythrocyte membrane protein band 3; Erythroid anion exchange protein; FR; Froese blood group; RTA1A; SLC4A1; Solute carrier family 4 anion exchanger member 1; Solute carrier family 4 member 1; SW; Swann blood group; Waldner blood group; WD; WD1; WR; Wright blood group;

Immunogens

Immunogen:

Uniprot:

P02730(B3AT_HUMAN) >>Visit HPA database.

Gene(ID):

SLC4A1(6521)

Expression:

P02730 B3AT_HUMAN:

Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).

Sequence:

P02730 B3AT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTSHPGTHKVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDREELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLFCEQGDGGTEGHSPSGILEKIPPDSEATLVLVGRADFLEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRIDAYMAQSRGELLHSLEGFLDCSLVLPPTDAPSEQALLSLVPVQRELLRRRYQSSPAKPDSSFYKGLDLNGGPDDPLQQTGQLFGGLVRDIRRRYPYYLSDITDAFSPQVLAAVIFIYFAALSPAITFGGLLGEKTRNQMGVSELLISTAVQGILFALLGAQPLLVVGFSGPLLVFEEAFFSFCETNGLEYIVGRVWIGFWLILLVVLVVAFEGSFLVRFISRYTQEIFSFLISLIFIYETFSKLIKIFQDHPLQKTYNYNVLMVPKPQGPLPNTALLSLVLMAGTFFFAMMLRKFKNSSYFPGKLRRVIGDFGVPISILIMVLVDFFIQDTYTQKLSVPDGFKVSNSSARGWVIHPLGLRSEFPIWMMFASALPALLVFILIFLESQITTLIVSKPERKMVKGSGFHLDLLLVVGMGGVAALFGMPWLSATTVRSVTHANALTVMGKASTPGAAAQIQEVKEQRISGLLVAVLVGLSILMEPILSRIPLAVLFGIFLYMGVTSLSGIQLFDRILLLFKPPKYHPDVPYVKRVKTWRMHLFTGIQIICLAVLWVVKSTPASLALPFVLILTVPLRRVLLPLIFRNVELQCLDADDAKATFDEEEGRDEYDEVAMPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Dog

Horse

Xenopus

Pig

Bovine

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02730 As Substrate

P02730

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
Y8	Phosphorylation	P43405 (SYK) , P07948 (LYN)	Uniprot
Y21	Phosphorylation	P43405 (SYK) , P07948 (LYN)	Uniprot
S29	Phosphorylation		Uniprot
T39	Phosphorylation		Uniprot
T42	Phosphorylation	P48729 (CSNK1A1)	Uniprot
T44	Phosphorylation		Uniprot
Y46	Phosphorylation		Uniprot
T48	Phosphorylation		Uniprot
T49	Phosphorylation		Uniprot
S50	Phosphorylation		Uniprot
T54	Phosphorylation		Uniprot
S162	O-Glycosylation		Uniprot
S181	Phosphorylation		Uniprot
S194	Phosphorylation		Uniprot
C201	S-Nitrosylation		Uniprot
S224	O-Glycosylation		Uniprot
S303	Phosphorylation	P48729 (CSNK1A1)	Uniprot
C317	S-Nitrosylation		Uniprot
Y347	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
S356	Phosphorylation		Uniprot
S357	Phosphorylation		Uniprot
Y359	Phosphorylation	P07948 (LYN) , P43405 (SYK)	Uniprot
S525	Phosphorylation		Uniprot
N642	N-Glycosylation		Uniprot
S745	O-Glycosylation		Uniprot
K757	Ubiquitination		Uniprot
S781	Phosphorylation		Uniprot
Y818	Phosphorylation		Uniprot
T894	Phosphorylation		Uniprot
Y904	Phosphorylation	P43405 (SYK) , P07948 (LYN)	Uniprot

Research Backgrounds

P02730_B3AT_HUMAN

Function:

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.

PTMs:

Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.

Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.

N-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein.
Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.

Tissue Specificity:

Detected in erythrocytes (at protein level). Isoform 2 is expressed in kidney (at protein level).

Subunit Structure:

A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM. Isoform 2 interacts with TM139.

Family&Domains:

Belongs to the anion exchanger (TC 2.A.31) family.

Research Fields

· Organismal Systems > Excretory system > Collecting duct acid secretion.

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Band 3/AE 1 Antibody - #DF7843