MCAM Antibody - #DF7846
| Product: | MCAM Antibody |
| Catalog: | DF7846 |
| Description: | Rabbit polyclonal antibody to MCAM |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 140 kd; 72kD(Calculated). |
| Uniprot: | P43121 |
| RRID: | AB_2841283 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7846, RRID:AB_2841283.
Fold/Unfold
A32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; Mcam; Melanoma adhesion molecule; Melanoma associated antigen A32; Melanoma associated antigen MUC18; Melanoma associated glycoprotein MUC18; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MelCAM; MUC 18; MUC18; MUC18_HUMAN; S endo 1; S endo 1 endothelial associated antigen; S-endo 1 endothelial-associated antigen;
Immunogens
Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
- P43121 MUC18_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLPRLVCAFLLAACCCCPRVAGVPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRGVVIVAVIVCILVLAVLGAVLYFLYKKGKLPCRRSGKQEITLPPSRKSELVVEVKSDKLPEEMGLLQGSSGDKRAPGDQGEKYIDLRH
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P43121 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S93 | Phosphorylation | Uniprot | |
| R97 | Methylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| Y126 | Phosphorylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| N467 | N-Glycosylation | Uniprot | |
| K585 | Acetylation | Uniprot | |
| K605 | Acetylation | Uniprot | |
| K605 | Ubiquitination | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| K613 | Acetylation | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| K616 | Ubiquitination | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| Y641 | Phosphorylation | Uniprot |
Research Backgrounds
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Membrane>Single-pass type I membrane protein.
Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
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