SCARF1 Antibody - #DF7922

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Product: SCARF1 Antibody
Catalog: DF7922
Description: Rabbit polyclonal antibody to SCARF1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Mol.Wt.: 87 kDa; 87kD(Calculated).
Uniprot: Q14162
RRID: AB_2841336

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Clonality:
Polyclonal
Specificity:
SCARF1 Antibody detects endogenous levels of total SCARF1.
RRID:
AB_2841336
Cite Format: Affinity Biosciences Cat# DF7922, RRID:AB_2841336.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acetyl LDL receptor; Endothelial cells scavenger receptor; SCARF1; Scavenger receptor class F member 1; Scavenger receptor expressed by endothelial cells 1; Scavenger receptor expressed by endothelial cells; SREC; SREC-I; SREC_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q14162(SREC_HUMAN) >>Visit HPA database.

Gene(ID):
SCARF1(8578)
Expression:
Q14162 SREC_HUMAN:

Endothelial cells.

Sequence:
MGLGLLLPLLLLWTRGTQGSELDPKGQHVCVASSPSAELQCCAGWRQKDQECTIPICEGPDACQKDEVCVKPGLCRCKPGFFGAHCSSRCPGQYWGPDCRESCPCHPHGQCEPATGACQCQADRWGARCEFPCACGPHGRCDPATGVCHCEPGWWSSTCRRPCQCNTAAARCEQATGACVCKPGWWGRRCSFRCNCHGSPCEQDSGRCACRPGWWGPECQQQCECVRGRCSAASGECTCPPGFRGARCELPCPAGSHGVQCAHSCGRCKHNEPCSPDTGSCESCEPGWNGTQCQQPCLPGTFGESCEQQCPHCRHGEACEPDTGHCQRCDPGWLGPRCEDPCPTGTFGEDCGSTCPTCVQGSCDTVTGDCVCSAGYWGPSCNASCPAGFHGNNCSVPCECPEGLCHPVSGSCQPGSGSRDTALIAGSLVPLLLLFLGLACCACCCWAPRSDLKDRPARDGATVSRMKLQVWGTLTSLGSTLPCRSLSSHKLPWVTVSHHDPEVPFNHSFIEPPSAGWATDDSFSSDPESGEADEVPAYCVPPQEGMVPVAQAGSSEASLAAGAFPPPEDASTPFAIPRTSSLARAKRPSVSFAEGTKFAPQSRRSSGELSSPLRKPKRLSRGAQSGPEGREAEESTGPEEAEAPESFPAAASPGDSATGHRRPPLGGRTVAEHVEAIEGSVQESSGPVTTIYMLAGKPRGSEGPVRSVFRHFGSFQKGQAEAKVKRAIPKPPRQALNRKKGSPGLASGSVGQSPNSAPKAGLPGATGPMAVRPEEAVRGLGAGTESSRRAQEPVSGCGSPEQDPQKQAEEERQEEPEYENVVPISRPPEP

PTMs - Q14162 As Substrate

Site PTM Type Enzyme
T473 Phosphorylation
S476 Phosphorylation
S589 Phosphorylation
S606 Phosphorylation
S611 Phosphorylation
Y692 Phosphorylation
S714 Phosphorylation
S753 Phosphorylation
S756 Phosphorylation
S799 Phosphorylation
Y818 Phosphorylation

Research Backgrounds

Function:

Mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Mediates heterophilic interactions, suggesting a function as adhesion protein. Plays a role in the regulation of neurite-like outgrowth (By similarity).

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Endothelial cells.

Subunit Structure:

Heterophilic interaction with SREC2 via its extracellular domain. The heterophilic interaction is suppressed by the presence of ligand such as Ac-LDL. Interacts with AVIL (By similarity).

References

1). The Role of SREC-Ⅰ in Innate Immunity to Aspergillus fumigatus Keratitis. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2021 (PubMed: 34236384) [IF=5.0]

Application: WB    Species: Human    Sample: HCECs

Figure 1. Expression of SREC-Ⅰ in normal and A fumigatus–treated HCECs and corneas. SREC-Ⅰ mRNA level was significantly higher in A fumigatus–stimulated HCECs at 8 hours comparing with control (A). Immunofluorescence staining for SREC-Ⅰ (green) in normal and A fumigatus–infected human corneas, and quantitative analysis (n = 6/group/time) (t-test). Scale bar = 50 µm. (B) SREC-Ⅰ protein expression level in A fumigatus–stimulated HCECs at 0, 8, 12, 24 and 36 hours (C) and quantitative analysis (D). Values represents means ± SD. *P < 0.05, **P < 0.001 compared with untreated cells by one-way ANOVA test.

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