MSLN Antibody | Affinity Biosciences

1/2

MSLN Antibody - Western blot analysis of MSLN using MCF7 whole lysates.

MSLN Antibody - DF7931 at 1/100 staining Human prostate cancer by IHC-P.

Product:	MSLN Antibody
Catalog:	DF7931
Description:	Rabbit polyclonal antibody to MSLN
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	69 kDa; 69kD(Calculated).
Uniprot:	Q13421
RRID:	AB_2841342

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

MSLN Antibody detects endogenous levels of total MSLN.

RRID:

AB_2841342
Cite Format: Affinity Biosciences Cat# DF7931, RRID:AB_2841342.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CAK 1; CAK1; CAK1 antigen; cleaved form; Megakaryocyte potentiating factor; Mesothelin; Mesothelin isoform 1 precursor; MPF; Msln; MSLN_HUMAN; Pre pro megakaryocyte potentiating factor; Pre-pro-megakaryocyte-potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein;

Immunogens

Immunogen:

Uniprot:

Q13421(MSLN_HUMAN) >>Visit HPA database.

Gene(ID):

MSLN(10232)

Expression:

Q13421 MSLN_HUMAN:

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

Sequence:

Q13421 MSLN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQEAAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALSGTPCLLGPGPVLTVLALLLASTLA

PTMs - Q13421 As Substrate

Q13421

Site	PTM Type	Source
K92	Ubiquitination	Uniprot
K95	Ubiquitination	Uniprot
S178	Phosphorylation	Uniprot
S200	Phosphorylation	Uniprot
S262	Phosphorylation	Uniprot
K319	Ubiquitination	Uniprot
K353	Ubiquitination	Uniprot
K386	Ubiquitination	Uniprot
K487	Ubiquitination	Uniprot
T530	Phosphorylation	Uniprot
K549	Ubiquitination	Uniprot

Research Backgrounds

Q13421_MSLN_HUMAN

Function:

Membrane-anchored forms may play a role in cellular adhesion.

Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

PTMs:

Both MPF and the cleaved form of mesothelin are N-glycosylated.

Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

Subcellular Location:

Cell membrane>Lipid-anchor. Golgi apparatus.

Secreted.

Secreted.

Tissue Specificity:

Subunit Structure:

Interacts with MUC16.

Family&Domains:

Belongs to the mesothelin family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MSLN Antibody - #DF7931