Product: ICAD Antibody
Catalog: DF7947
Description: Rabbit polyclonal antibody to ICAD
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Horse
Mol.Wt.: 37 kDa; 37kD(Calculated).
Uniprot: O00273
RRID: AB_2841349

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(82%)
Clonality:
Polyclonal
Specificity:
ICAD Antibody detects endogenous levels of total ICAD.
RRID:
AB_2841349
Cite Format: Affinity Biosciences Cat# DF7947, RRID:AB_2841349.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A330085O09Rik; Caspase activated deoxyribonuclease inhibitor short form; DFF 1; DFF 45; DFF alpha; DFF-45; DFF1; DFF35; DFF45; DFFA; Dffa DNA fragmentation factor, alpha subunit; DFFA_HUMAN; DNA fragmentation factor 45 kDa subunit; DNA Fragmentation Factor Alpha Subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45 kD, alpha subunit; DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, alpha subunit; DNAation factor 45 kDa subunit; H13; ICAD; ICAD L; ICAD S; Inhibitor of CAD; Inhibitor of Caspase Activated DNase; MGC143066; OTTHUMP00000001903; OTTHUMP00000001904; RP23 121D17.3;

Immunogens

Immunogen:

A synthesized peptide derived from human ICAD, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
82
Pig
73
Bovine
73
Sheep
73
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Inhibitor of the caspase-activated DNase (DFF40).

PTMs:

Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

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